##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064214_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	921514
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260284705387004	32.0	32.0	32.0	32.0	32.0
2	31.38772498301708	32.0	32.0	32.0	32.0	32.0
3	31.456885082592343	32.0	32.0	32.0	32.0	32.0
4	31.542994463459046	32.0	32.0	32.0	32.0	32.0
5	31.488693606391223	32.0	32.0	32.0	32.0	32.0
6	35.060124968258755	36.0	36.0	36.0	36.0	36.0
7	35.08602039686863	36.0	36.0	36.0	36.0	36.0
8	35.03103154157181	36.0	36.0	36.0	36.0	36.0
9	35.14016173384235	36.0	36.0	36.0	36.0	36.0
10	34.98865671058714	36.0	36.0	36.0	36.0	36.0
11	35.1375627499962	36.0	36.0	36.0	36.0	36.0
12	35.036323919115716	36.0	36.0	36.0	36.0	36.0
13	35.08612348808591	36.0	36.0	36.0	36.0	36.0
14	35.017155463726	36.0	36.0	36.0	36.0	36.0
15	34.99657194573278	36.0	36.0	36.0	36.0	36.0
16	35.00505581032952	36.0	36.0	36.0	36.0	36.0
17	34.96701949183626	36.0	36.0	36.0	36.0	36.0
18	34.964149215313064	36.0	36.0	36.0	36.0	36.0
19	34.96347206879114	36.0	36.0	36.0	36.0	36.0
20	34.940924391816075	36.0	36.0	36.0	36.0	36.0
21	34.955698991008276	36.0	36.0	36.0	36.0	36.0
22	34.94533561074493	36.0	36.0	36.0	36.0	36.0
23	34.88177282168258	36.0	36.0	36.0	32.0	36.0
24	34.85974385630603	36.0	36.0	36.0	32.0	36.0
25	34.8334566810705	36.0	36.0	36.0	32.0	36.0
26	34.76960523659977	36.0	36.0	36.0	32.0	36.0
27	34.770397411216756	36.0	36.0	36.0	32.0	36.0
28	34.73927580047617	36.0	36.0	36.0	32.0	36.0
29	34.71112647230536	36.0	36.0	36.0	32.0	36.0
30	34.69011105637028	36.0	36.0	36.0	32.0	36.0
31	34.683262544030804	36.0	36.0	36.0	32.0	36.0
32	34.63785249057529	36.0	36.0	36.0	32.0	36.0
33	34.60890122125112	36.0	36.0	36.0	32.0	36.0
34	34.59263342716443	36.0	36.0	36.0	32.0	36.0
35	34.541604359781836	36.0	36.0	36.0	32.0	36.0
36	34.5095364801837	36.0	36.0	36.0	32.0	36.0
37	34.496116174035336	36.0	36.0	36.0	32.0	36.0
38	34.45482759892959	36.0	36.0	36.0	32.0	36.0
39	34.439304231948725	36.0	36.0	36.0	32.0	36.0
40	34.42173206267078	36.0	36.0	36.0	32.0	36.0
41	34.40350119477295	36.0	36.0	36.0	32.0	36.0
42	34.32842908517939	36.0	36.0	36.0	32.0	36.0
43	34.31409615046543	36.0	36.0	36.0	32.0	36.0
44	34.24259099698973	36.0	36.0	36.0	32.0	36.0
45	34.205731003544166	36.0	36.0	36.0	32.0	36.0
46	34.16373272679525	36.0	36.0	36.0	32.0	36.0
47	34.128973623840764	36.0	36.0	36.0	32.0	36.0
48	34.07677582760544	36.0	36.0	36.0	32.0	36.0
49	34.047369871754526	36.0	36.0	36.0	32.0	36.0
50	33.45009082878828	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	7.0
20	13.0
21	33.0
22	94.0
23	314.0
24	863.0
25	1963.0
26	3959.0
27	7225.0
28	11722.0
29	17573.0
30	25082.0
31	34309.0
32	49782.0
33	78872.0
34	158609.0
35	531093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73131995858647	17.93533585547807	11.58667213637814	26.746672049557326
2	15.982337704053451	20.771103112171204	36.74747235789915	26.499086825876194
3	18.413779108442547	24.43920908160619	28.63622897610555	28.510782833845717
4	12.527427689649858	15.97555761496841	35.57797277089659	35.91904192448514
5	14.218774755456781	36.8923315326734	33.33622712188855	15.552666589981271
6	34.04336129821283	35.5627104555226	16.680828159776368	13.7131000864882
7	29.978925984846676	30.406808795091557	20.996316930616356	18.617948289445412
8	28.04819026080993	32.9920109732462	19.535568640302806	19.424230125641063
9	27.701748769675692	13.828790945247176	18.42496785150379	40.04449243357334
10	15.71192933337674	26.890755390608888	32.006163796377685	25.39115147963668
11	37.36004010790938	20.91015437638495	22.589456047330806	19.140349468374872
12	24.739368276667342	23.640303805380512	29.05478068086002	22.565547237092122
13	29.845286372677858	19.457758958404096	25.302737140928627	25.394217527989415
14	23.541878501721627	19.444803154818555	25.656666062586343	31.356652280873476
15	25.130088932538243	27.154213036565977	22.520822368242566	25.194875662653217
16	25.38333988058678	25.583121542344813	24.2334830158458	24.80005556122261
17	23.80886237213976	25.67318564883442	25.6055795137133	24.91237246531252
18	24.539778103811997	24.897559662815024	26.659121096632493	23.90354113674049
19	25.490687597461996	24.653260453189482	25.754058814145868	24.10199313520265
20	25.67598509832264	24.189506970646995	25.52406004512174	24.610447885908627
21	26.489234021403906	23.996705421729892	25.074822520330674	24.439238036535528
22	25.612009814294534	24.145725562200422	25.47495260511789	24.767312018387152
23	24.484511749800596	24.18923391625656	25.87180753224345	25.454446801699394
24	24.784104350468255	24.782910657507788	25.7487167800675	24.68426821195646
25	24.917226897595754	24.359003575711206	25.89010249648669	24.83366703020635
26	24.43494746940463	25.178764638406538	26.160247345796844	24.22604054639199
27	24.923165077876753	24.68658297303398	25.533829070514493	24.856422878574776
28	24.424888819434948	24.72864128559243	26.021786832835218	24.8246830621374
29	24.33284463047227	24.81358933983876	25.832594491295048	25.020971538393923
30	24.179827517165265	25.209906205540417	26.07351410715119	24.536752170143128
31	24.848065219089953	24.748873512124394	25.26588586659258	25.13717540219307
32	24.48775839972045	25.124553847088787	25.223090020607952	25.16459773258281
33	24.229220701704886	24.724516262059534	25.71044092591186	25.33582211032372
34	24.942267289427534	24.798152553261684	25.79751445488167	24.462065702429115
35	25.228978473853047	24.743023210386724	25.84960780653815	24.17839050922208
36	24.411448390972122	25.291793322965695	25.463912043643926	24.832846242418256
37	25.031796536890493	25.066957212496366	25.2904011355596	24.610845115053547
38	24.484070654320828	25.072060808904283	25.53047173783757	24.91339679893732
39	25.07241108006186	24.74825687077783	25.258525733199487	24.92080631596082
40	25.202824127932217	25.078568576042226	25.601635615439378	24.116971680586182
41	24.36597250105807	25.107923037688963	26.102833454513885	24.423271006739085
42	25.213836600839066	25.499892566234543	25.39864438121407	23.887626451712322
43	24.49473903531618	24.782420911900545	25.591424453936174	25.131415598847102
44	24.264812007392305	25.339365533766177	25.281742031190486	25.114080427651036
45	24.359631036353772	25.378838849701573	25.36603364080304	24.895496473141616
46	24.26150378567142	25.094492572444306	25.370780932413528	25.273222709470744
47	24.498448709754083	25.08391291938825	25.584189102226716	24.833449268630947
48	24.912859090001476	25.54216187590613	24.780465677550417	24.76451335654197
49	24.347808157932434	25.637433424343577	25.02561019546222	24.98914822226177
50	24.453772211020702	25.900866194538917	24.886218369574888	24.75914322486549
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	178.0
1	124.0
2	70.0
3	183.5
4	297.0
5	351.5
6	406.0
7	375.0
8	344.0
9	419.0
10	494.0
11	771.0
12	1048.0
13	1733.5
14	2419.0
15	3035.5
16	3652.0
17	3791.5
18	3931.0
19	3890.0
20	3849.0
21	4184.0
22	4519.0
23	4989.0
24	5459.0
25	5858.5
26	6258.0
27	7576.5
28	8895.0
29	10326.0
30	11757.0
31	13450.5
32	15144.0
33	17706.0
34	20268.0
35	23085.0
36	25902.0
37	29536.0
38	33170.0
39	35981.0
40	38792.0
41	41999.0
42	45206.0
43	46909.0
44	48612.0
45	53136.0
46	57660.0
47	61321.5
48	64983.0
49	67541.5
50	70100.0
51	68692.0
52	67284.0
53	67333.0
54	67382.0
55	67104.0
56	66826.0
57	64659.5
58	62493.0
59	57231.5
60	51970.0
61	45935.5
62	39901.0
63	34651.5
64	29402.0
65	25115.0
66	20828.0
67	17706.0
68	14584.0
69	12750.5
70	10917.0
71	8421.0
72	5925.0
73	5109.5
74	4294.0
75	3271.0
76	2248.0
77	1914.0
78	1580.0
79	1335.5
80	1091.0
81	866.0
82	641.0
83	520.0
84	399.0
85	275.0
86	151.0
87	109.5
88	68.0
89	55.0
90	42.0
91	35.5
92	29.0
93	22.5
94	16.0
95	13.0
96	10.0
97	13.0
98	16.0
99	10.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008247297382351217
2	3.2555121246123224E-4
3	3.2555121246123224E-4
4	0.0
5	0.0
6	1.0851707082041077E-4
7	0.0
8	0.0
9	9.766536373836969E-4
10	4.3406828328164306E-4
11	0.0
12	3.2555121246123224E-4
13	5.425853541020538E-4
14	3.2555121246123224E-4
15	0.003146995053791912
16	8.681365665632861E-4
17	0.0
18	2.1703414164082153E-4
19	1.0851707082041077E-4
20	7.596194957428754E-4
21	0.0
22	1.0851707082041077E-4
23	9.766536373836969E-4
24	4.3406828328164306E-4
25	0.0020618243455878043
26	0.005534370611840949
27	0.006293990107583824
28	0.004340682832816431
29	0.0029299609121510905
30	0.0033640291954327335
31	0.00716212667414711
32	0.0027129267705102694
33	0.004774751116098073
34	0.00282144384133068
35	0.003255512124612323
36	0.006185473036763414
37	0.003255512124612323
38	0.007596194957428753
39	0.00423216576199602
40	0.00282144384133068
41	0.002604409699689858
42	0.0017362731331265722
43	0.0010851707082041077
44	0.0016277560623061615
45	0.0015192389914857507
46	0.00141072192066534
47	0.0024958926288694474
48	0.0019533072747673938
49	6.511024249224645E-4
50	0.001302204849844929
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	921514.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.80302228839643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.25015223750879	48.97898925093565
2	12.929852565991059	15.982079326432503
3	3.69232117146763	6.845898229683958
4	1.4920129077792719	3.6884362797623
5	0.7432148012854024	2.296646046445392
6	0.4417278830555198	1.6380070921131873
7	0.2828752934452788	1.223778364594467
8	0.20933630858039382	1.0350093235971767
9	0.14909178896338415	0.8292890840688834
>10	0.6964002260441945	7.57606379124894
>50	0.057306903193188645	2.5256094053778386
>100	0.05270145402543723	6.199874339240252
>500	0.0030064586604360054	1.1803194664994963
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.0851707082041077E-4	0.0
13	0.0	1.0851707082041077E-4	0.0	1.0851707082041077E-4	1.0851707082041077E-4
14	0.0	1.0851707082041077E-4	0.0	1.0851707082041077E-4	1.0851707082041077E-4
15	0.0	1.0851707082041077E-4	0.0	2.1703414164082153E-4	1.0851707082041077E-4
16	0.0	1.0851707082041077E-4	0.0	2.1703414164082153E-4	1.0851707082041077E-4
17	0.0	1.0851707082041077E-4	0.0	3.255512124612323E-4	1.0851707082041077E-4
18	0.0	1.0851707082041077E-4	0.0	3.255512124612323E-4	1.0851707082041077E-4
19	0.0	1.0851707082041077E-4	0.0	3.255512124612323E-4	1.0851707082041077E-4
20	0.0	1.0851707082041077E-4	0.0	3.255512124612323E-4	2.1703414164082153E-4
21	0.0	1.0851707082041077E-4	0.0	5.425853541020538E-4	2.1703414164082153E-4
22	0.0	1.0851707082041077E-4	0.0	0.0010851707082041077	3.255512124612323E-4
23	0.0	1.0851707082041077E-4	0.0	0.002278858487228626	4.3406828328164306E-4
24	0.0	1.0851707082041077E-4	0.0	0.0033640291954327335	4.3406828328164306E-4
25	0.0	1.0851707082041077E-4	0.0	0.004340682832816431	4.3406828328164306E-4
26	0.0	1.0851707082041077E-4	0.0	0.00575140475348177	5.425853541020538E-4
27	0.0	1.0851707082041077E-4	0.0	0.007487677886608342	5.425853541020538E-4
28	0.0	1.0851707082041077E-4	0.0	0.012370946073526826	5.425853541020538E-4
29	0.0	1.0851707082041077E-4	0.0	0.021052311739159685	5.425853541020538E-4
30	0.0	1.0851707082041077E-4	0.0	0.04047686741601321	5.425853541020538E-4
31	0.0	1.0851707082041077E-4	0.0	0.08225593968187135	5.425853541020538E-4
32	0.0	1.0851707082041077E-4	0.0	0.1282671777097255	5.425853541020538E-4
33	0.0	1.0851707082041077E-4	0.0	0.17525506937496338	5.425853541020538E-4
34	0.0	1.0851707082041077E-4	0.0	0.22517292195235233	6.511024249224646E-4
35	0.0	2.1703414164082153E-4	0.0	0.27899738907927607	6.511024249224646E-4
36	0.0	2.1703414164082153E-4	0.0	0.35995112391130246	6.511024249224646E-4
37	0.0	2.1703414164082153E-4	0.0	0.4774751116098073	6.511024249224646E-4
38	0.0	2.1703414164082153E-4	0.0	0.6440488153191378	6.511024249224646E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTCA	65	4.9316767E-4	20.307243	10
CGAAATT	55	0.0044819852	20.00064	28
TACGACG	265	0.0	19.923006	5
CGACGGT	275	0.0	19.198532	7
ACGACGG	275	0.0	19.198532	6
GAGTGCG	70	8.125498E-4	18.8557	7
GTGTAGG	190	1.8189894E-12	18.526909	1
ACGGTAT	285	0.0	18.525906	9
GACGGTA	290	0.0	18.205505	8
CGAATGC	220	0.0	17.999601	43
GTATCAA	2655	0.0	17.981743	1
CCGGTCC	250	0.0	17.59961	9
AACGCAG	2780	0.0	17.092218	6
CTTTCGT	310	0.0	17.033728	38
GATATAC	195	4.9112714E-11	16.923618	1
TCTAGCG	235	0.0	16.851604	28
TAGGACC	405	0.0	16.838219	4
GTAGGAC	275	0.0	16.798716	3
ATACGAA	225	1.8189894E-12	16.621853	40
TTCGTTC	320	0.0	16.499634	40
>>END_MODULE