FastQCFastQC Report
Thu 2 Feb 2017
SRR4064213_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064213_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322914
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44400.33562272377493924No Hit
TATCAACGCAGAGTACTTTTTTTTT30990.23425559031048124No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26480.20016418300811695No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18550.14022075509065593No Hit
GCGCAAGACGGACCAGAGCGAAAGC17220.13016719151811834No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16350.12359080030901479No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15880.12003803724202783No Hit
GAACTACGACGGTATCTGATCGTCT15180.11474668799332383No Hit
GAATAGGACCGCGGTTCTATTTTGT15150.11451991588266508No Hit
GATTAAGAGGGACGGCCGGGGGCAT14740.1114206970369956No Hit
GTATCTGATCGTCTTCGAACCTCCG14660.11081597140857229No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14640.11066479000146646No Hit
CTATTGGAGCTGGAATTACCGCGGC14510.10968211085527857No Hit
GGTATCAACGCAGAGTACTTTTTTT14240.1076411618593499No Hit
ATCAGATACCGTCGTAGTTCCGACC13740.10386162668170418No Hit
GTCCTATTCCATTATTCCTAGCTGC13660.10325690105328086No Hit
TCGTAGTTCCGACCATAAACGATGC13400.10129154276090509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGA500.001498327713.30119210
AGGCCCG3500.012.758285510
CGCGTAA1207.385097E-1012.66780210
GCGTTAT1207.4396667E-1012.6615721
CAAGACG3350.012.4749544
GTATTTA1103.8327926E-812.0860461
CCGTCAA2050.012.05168418
ACGGTAT2450.012.0214869
CGAGCCG3450.011.84253715
CCGACCA2650.011.8312499
AAGACGG3300.011.8009625
CGCAAGA2900.011.7879142
CGTCAAT2100.011.7656319
TCGCGTA1302.6084308E-911.6933569
GCGTAAC1302.6084308E-911.69335611
GTATTAT658.0491445E-411.6876061
TTACAGC907.4757936E-611.6086384
CGACCAT2950.011.5942610
CGAACGA1157.052586E-811.5671316
TCCGTCA2150.011.49027217
Produced by FastQC (version 0.11.5)