##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064213_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1322914
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31243225183194	32.0	32.0	32.0	32.0	32.0
2	31.08541825092183	32.0	32.0	32.0	32.0	32.0
3	31.109205889422896	32.0	32.0	32.0	32.0	32.0
4	31.11109187747654	32.0	32.0	32.0	32.0	32.0
5	31.091801885836873	32.0	32.0	32.0	32.0	32.0
6	34.66248977635735	36.0	36.0	36.0	32.0	36.0
7	34.626929641684946	36.0	36.0	36.0	32.0	36.0
8	34.6088294477192	36.0	36.0	36.0	32.0	36.0
9	34.68218871370323	36.0	36.0	36.0	32.0	36.0
10	34.5144695724741	36.0	36.0	36.0	32.0	36.0
11	34.65250802395318	36.0	36.0	36.0	32.0	36.0
12	34.546573700180055	36.0	36.0	36.0	32.0	36.0
13	34.610548380317994	36.0	36.0	36.0	32.0	36.0
14	34.530478171672534	36.0	36.0	36.0	32.0	36.0
15	34.48686082390843	36.0	36.0	36.0	32.0	36.0
16	34.47908556414098	36.0	36.0	36.0	32.0	36.0
17	34.41800147250691	36.0	36.0	36.0	32.0	36.0
18	34.40933953378678	36.0	36.0	36.0	32.0	36.0
19	34.411314718870614	36.0	36.0	36.0	32.0	36.0
20	34.37512642545169	36.0	36.0	36.0	32.0	36.0
21	34.347798874303244	36.0	36.0	36.0	32.0	36.0
22	34.33961769245771	36.0	36.0	36.0	32.0	36.0
23	34.315295627682524	36.0	36.0	36.0	32.0	36.0
24	34.28783276917471	36.0	36.0	36.0	32.0	36.0
25	33.821817593585074	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	12.0
5	16.0
6	51.0
7	17.0
8	78.0
9	100.0
10	117.0
11	34.0
12	85.0
13	79.0
14	162.0
15	304.0
16	521.0
17	663.0
18	778.0
19	1048.0
20	1315.0
21	1741.0
22	2517.0
23	3493.0
24	4981.0
25	7095.0
26	10074.0
27	13823.0
28	19511.0
29	27620.0
30	38618.0
31	55104.0
32	83344.0
33	126887.0
34	299550.0
35	623172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65179745568086	17.21359016997165	11.30847997991526	25.826132394432232
2	16.795656039091416	19.868571445850165	37.14009470475822	26.1956778103002
3	18.839472128614602	23.91499571395527	28.552314690928554	28.693217466501576
4	12.60536789468313	15.116508487260605	35.71490666092156	36.563216957134706
5	14.742753812181594	36.340139356017055	33.38587343957774	15.531233392223612
6	34.19944674986335	35.31335594819498	16.865283818529722	13.621913483411946
7	30.254824024515365	30.411787699824362	20.948753174620578	18.384635101039695
8	28.25614675846633	32.41556359621761	19.62879363603023	19.69949600928583
9	27.445449127700318	13.963450234902272	18.78590465357587	39.80519598382154
10	16.003539702528847	26.36412798898759	31.345427316973556	26.286904991510006
11	37.36668073410871	20.947631299907275	22.58913539686069	19.096552569123325
12	24.850955683803676	23.596805058657125	28.742899500034035	22.80933975750516
13	29.17410471153497	19.21995836711971	25.71601897684776	25.889917944497558
14	23.637089795646677	19.657585641410442	25.257710903886764	31.447613659056113
15	25.019722038385172	27.196596388389903	22.504982509218117	25.27869906400681
16	25.681008315135546	25.625790913636205	23.919724487858602	24.773476283369654
17	23.857525153431773	25.70816119374988	25.441007619776624	24.993306033041723
18	24.77086359235052	24.60042967804406	26.555389130961025	24.073317598644394
19	25.35385253137602	24.82758412011356	25.65726816995209	24.161295178558326
20	25.715960798701865	24.07278944242924	25.15071166771945	25.060538091149446
21	26.79944536438244	23.994874234936447	24.727731553940433	24.47794884674068
22	25.740987155793967	24.12886114807337	25.30147459867057	24.828677097462098
23	24.241126714533525	23.905312283212833	25.88101169487563	25.972549307378006
24	24.568225023015643	24.916126230873566	25.638480793568785	24.87716795254201
25	24.716716077606065	24.384241856651034	25.60830678708865	25.290735278654246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	191.0
1	191.0
2	454.0
3	717.0
4	717.0
5	717.0
6	2109.5
7	3502.0
8	3502.0
9	3502.0
10	3480.5
11	3459.0
12	3459.0
13	3459.0
14	3453.0
15	3447.0
16	3447.0
17	3447.0
18	5919.5
19	8392.0
20	8392.0
21	8392.0
22	13741.0
23	19090.0
24	19090.0
25	19090.0
26	28614.5
27	38139.0
28	38139.0
29	38139.0
30	47514.5
31	56890.0
32	56890.0
33	56890.0
34	69522.5
35	82155.0
36	82155.0
37	82155.0
38	93358.5
39	104562.0
40	104562.0
41	104562.0
42	120784.5
43	137007.0
44	137007.0
45	137007.0
46	154382.0
47	171757.0
48	171757.0
49	171757.0
50	176286.5
51	180816.0
52	180816.0
53	180816.0
54	166918.0
55	153020.0
56	153020.0
57	153020.0
58	140818.5
59	128617.0
60	128617.0
61	128617.0
62	113337.5
63	98058.0
64	98058.0
65	98058.0
66	80895.0
67	63732.0
68	63732.0
69	63732.0
70	47992.0
71	32252.0
72	32252.0
73	32252.0
74	24894.5
75	17537.0
76	17537.0
77	17537.0
78	14475.5
79	11414.0
80	11414.0
81	11414.0
82	7767.0
83	4120.0
84	4120.0
85	4120.0
86	3170.0
87	2220.0
88	2220.0
89	2220.0
90	1531.5
91	843.0
92	843.0
93	843.0
94	579.5
95	316.0
96	316.0
97	316.0
98	488.5
99	661.0
100	661.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039080393736856664
2	0.0049889864344923405
3	0.001209451256846628
4	0.003703944474092798
5	0.00869293090858514
6	0.013984280157289137
7	0.02381107161916799
8	0.03545203996631678
9	0.05004104575202924
10	0.058129251032191055
11	0.05382058092967495
12	0.06228673972760135
13	0.06667100053367037
14	0.06727572616209368
15	0.05964106510324935
16	0.06553713998037666
17	0.062589102541813
18	0.07513715933159677
19	0.07294502892856225
20	0.07702692692041962
21	0.07294502892856225
22	0.07521275003514968
23	0.0795970108412187
24	0.07460802440672637
25	0.07611983847778464
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1322914.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.881583523109164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.98831364475814	35.90559203038593
2	14.290984938198983	13.685499778917393
3	4.604602902483058	6.614270353979802
4	2.0315142663409183	3.89088480088762
5	1.0616048127139006	2.541565975424764
6	0.6386753054103893	1.8348470988092886
7	0.42312417277306436	1.4181898793495966
8	0.3004743176473967	1.1509748909586446
9	0.22558763680191354	0.9721343945980473
>10	1.2154748115354443	10.524924412665786
>50	0.10122076629854701	3.367246136510602
>100	0.10082552806683893	10.401306777743612
>500	0.012999509474382243	4.257545661777693
>1k	0.004597387497037622	3.435017807991334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4440	0.33562272377493924	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3099	0.23425559031048124	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2648	0.20016418300811695	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1855	0.14022075509065593	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1722	0.13016719151811834	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1635	0.12359080030901479	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1588	0.12003803724202783	No Hit
GAACTACGACGGTATCTGATCGTCT	1518	0.11474668799332383	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1515	0.11451991588266508	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1474	0.1114206970369956	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1466	0.11081597140857229	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1464	0.11066479000146646	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1451	0.10968211085527857	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1424	0.1076411618593499	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1374	0.10386162668170418	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1366	0.10325690105328086	No Hit
TCGTAGTTCCGACCATAAACGATGC	1340	0.10129154276090509	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.511814071058285E-4
2	0.0	0.0	0.0	0.0	1.511814071058285E-4
3	0.0	0.0	0.0	0.0	1.511814071058285E-4
4	0.0	0.0	0.0	0.0	1.511814071058285E-4
5	0.0	0.0	0.0	0.0	1.511814071058285E-4
6	0.0	0.0	0.0	0.0	1.511814071058285E-4
7	0.0	0.0	0.0	0.0	1.511814071058285E-4
8	0.0	0.0	0.0	0.0	1.511814071058285E-4
9	0.0	0.0	0.0	0.0	1.511814071058285E-4
10	0.0	0.0	0.0	7.559070355291425E-5	1.511814071058285E-4
11	0.0	0.0	0.0	7.559070355291425E-5	1.511814071058285E-4
12	0.0	0.0	0.0	7.559070355291425E-5	3.779535177645712E-4
13	0.0	0.0	0.0	7.559070355291425E-5	4.535442213174855E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGA	50	0.0014983277	13.301192	10
AGGCCCG	350	0.0	12.7582855	10
CGCGTAA	120	7.385097E-10	12.667802	10
GCGTTAT	120	7.4396667E-10	12.661572	1
CAAGACG	335	0.0	12.474954	4
GTATTTA	110	3.8327926E-8	12.086046	1
CCGTCAA	205	0.0	12.051684	18
ACGGTAT	245	0.0	12.021486	9
CGAGCCG	345	0.0	11.842537	15
CCGACCA	265	0.0	11.831249	9
AAGACGG	330	0.0	11.800962	5
CGCAAGA	290	0.0	11.787914	2
CGTCAAT	210	0.0	11.76563	19
TCGCGTA	130	2.6084308E-9	11.693356	9
GCGTAAC	130	2.6084308E-9	11.693356	11
GTATTAT	65	8.0491445E-4	11.687606	1
TTACAGC	90	7.4757936E-6	11.608638	4
CGACCAT	295	0.0	11.59426	10
CGAACGA	115	7.052586E-8	11.56713	16
TCCGTCA	215	0.0	11.490272	17
>>END_MODULE