FastQCFastQC Report
Thu 2 Feb 2017
SRR4064213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322914
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16050.12132307920242737No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15550.11754354402478166No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15540.11746795332122874No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14640.11066479000146646No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA14380.10869943170909069No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA14340.10839706889487902No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14010.10590257567763285No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13950.10544903145631537No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC13850.10469312442078624No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13510.10212304049998715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGT250.002353179735.19906617
CTACTAG654.9346447E-420.3063851
GTATCAA34400.019.7603551
CTAGCGG4400.019.50021729
GTGTACG708.127398E-418.8559281
CGTATAA600.007413602418.33284444
CGTAACA600.00741683618.3314572
CGCAATA4700.018.25621236
TAGTACT851.4324971E-418.115114
ATACGAA4750.018.06335840
CCTAATA1002.4854819E-517.5982022
ACCGTCG3000.017.5975368
GTAAACG2900.017.45044927
AACGCAG38700.017.3929146
TAGCGGC4950.017.33418330
TCTAGCG5000.017.1614928
CGAATGC4900.017.0607743
TACCGTC3000.016.8643057
TACGAAT5100.016.82308241
CGTCGTA3400.016.82244710
Produced by FastQC (version 0.11.5)