##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064213_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1322914
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253810149412583	32.0	32.0	32.0	32.0	32.0
2	31.292817220166995	32.0	32.0	32.0	32.0	32.0
3	31.407835278786074	32.0	32.0	32.0	32.0	32.0
4	31.513223837679547	32.0	32.0	32.0	32.0	32.0
5	31.41976802724894	32.0	32.0	32.0	32.0	32.0
6	35.03060440814747	36.0	36.0	36.0	36.0	36.0
7	35.054670976344646	36.0	36.0	36.0	36.0	36.0
8	34.99662185145822	36.0	36.0	36.0	36.0	36.0
9	35.13596424257359	36.0	36.0	36.0	36.0	36.0
10	34.95555795766013	36.0	36.0	36.0	36.0	36.0
11	35.12819427415539	36.0	36.0	36.0	36.0	36.0
12	35.01263649791294	36.0	36.0	36.0	36.0	36.0
13	35.0693076042736	36.0	36.0	36.0	36.0	36.0
14	35.006337524585874	36.0	36.0	36.0	36.0	36.0
15	34.97305947325374	36.0	36.0	36.0	36.0	36.0
16	34.9807432682699	36.0	36.0	36.0	36.0	36.0
17	34.9349368137309	36.0	36.0	36.0	32.0	36.0
18	34.946852176331944	36.0	36.0	36.0	36.0	36.0
19	34.942371915332366	36.0	36.0	36.0	36.0	36.0
20	34.935257318313965	36.0	36.0	36.0	32.0	36.0
21	34.9318391066993	36.0	36.0	36.0	32.0	36.0
22	34.92226630000136	36.0	36.0	36.0	32.0	36.0
23	34.85747070482284	36.0	36.0	36.0	32.0	36.0
24	34.83034195722473	36.0	36.0	36.0	32.0	36.0
25	34.80051840104497	36.0	36.0	36.0	32.0	36.0
26	34.72789463260651	36.0	36.0	36.0	32.0	36.0
27	34.74098467474076	36.0	36.0	36.0	32.0	36.0
28	34.71111500823183	36.0	36.0	36.0	32.0	36.0
29	34.67511871519993	36.0	36.0	36.0	32.0	36.0
30	34.65483621762261	36.0	36.0	36.0	32.0	36.0
31	34.65435999619023	36.0	36.0	36.0	32.0	36.0
32	34.61195890284629	36.0	36.0	36.0	32.0	36.0
33	34.58065603659799	36.0	36.0	36.0	32.0	36.0
34	34.56802558594134	36.0	36.0	36.0	32.0	36.0
35	34.51714321565876	36.0	36.0	36.0	32.0	36.0
36	34.493706318022184	36.0	36.0	36.0	32.0	36.0
37	34.480579236443184	36.0	36.0	36.0	32.0	36.0
38	34.42645931632744	36.0	36.0	36.0	32.0	36.0
39	34.43542361786178	36.0	36.0	36.0	32.0	36.0
40	34.40062241385306	36.0	36.0	36.0	32.0	36.0
41	34.3769647913621	36.0	36.0	36.0	32.0	36.0
42	34.29242717213666	36.0	36.0	36.0	32.0	36.0
43	34.293253378526494	36.0	36.0	36.0	32.0	36.0
44	34.218863055346	36.0	36.0	36.0	32.0	36.0
45	34.17982045696092	36.0	36.0	36.0	32.0	36.0
46	34.15475835919795	36.0	36.0	36.0	32.0	36.0
47	34.110440285611915	36.0	36.0	36.0	32.0	36.0
48	34.0664842914959	36.0	36.0	36.0	32.0	36.0
49	34.042566636984716	36.0	36.0	36.0	32.0	36.0
50	33.3807964841252	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	0.0
20	10.0
21	36.0
22	105.0
23	346.0
24	975.0
25	2271.0
26	4771.0
27	8915.0
28	15314.0
29	24203.0
30	35103.0
31	51343.0
32	76164.0
33	125930.0
34	259869.0
35	717556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.47012598228764	18.29937141150812	12.018778277977479	26.211724328226758
2	16.005369996054142	20.636840410429766	36.54802382028655	26.809765773229543
3	18.07070629952627	24.503953030782917	28.87553112118823	28.54980954850258
4	12.21016634490224	15.74040338223044	35.917678700202735	36.13175157266459
5	14.372514010736904	36.759078821450224	33.11129824009724	15.757108927715635
6	34.44648539961147	35.67627427413807	16.45743096658125	13.419809359669213
7	30.152300149518414	30.587627011279643	20.925547692442592	18.33452514675935
8	28.303578312724788	32.59886886071203	19.416152523898	19.68140030266518
9	27.563307884193815	13.740645551440018	18.611308488925847	40.08473807544032
10	15.909879468892088	26.55872159166071	31.39629373457455	26.135105204872644
11	37.60602549071632	20.976814045972787	22.47494733213749	18.942213131173403
12	24.867016954251717	23.57320549423885	28.715947307192536	22.843830244316898
13	29.205218216892533	19.217872196319615	25.76014586092415	25.816763725863705
14	23.701972392700643	19.663907851699776	25.225582068635006	31.408537686964582
15	25.04951423137282	27.315829737567014	22.466156455899828	25.168499575160336
16	25.801367455466988	25.638164782541324	23.878917072027637	24.68155068996406
17	23.939349043097284	25.826546547999342	25.27594386331991	24.958160545583464
18	24.871193246413974	24.58077332430275	26.49632023898793	24.05171319029535
19	25.48396947949753	24.7373601005054	25.647925715503806	24.130744704493264
20	25.860130968947974	24.003767454904306	25.177771108972724	24.958330467174992
21	26.96055828270016	23.972079817735693	24.764270390970236	24.30309150859391
22	25.89509044826145	24.058383368193326	25.227093886134444	24.819432297410785
23	24.256138435883752	23.80205821479272	25.888090806973196	26.053712542350333
24	24.76135978810382	24.875048378123413	25.513412834716142	24.850178999056627
25	24.94829509891964	24.185043072299177	25.696579917332627	25.170081911448555
26	24.437026067380735	25.290492205790414	25.966763957270757	24.305717769558093
27	25.265525191820693	24.508825641607135	25.280114903428203	24.94553426314397
28	24.35323599336284	24.625147880909097	26.013811037490882	25.007805088237184
29	24.37407635584348	24.597754150965503	25.949269966777155	25.07889952641386
30	24.215283924990192	25.054068171630256	26.134448890175655	24.596199013203897
31	24.95046585905165	24.75172452894374	25.004894827358115	25.2929147846465
32	24.70560399371673	24.88302020292121	24.8050840667091	25.606291736652963
33	24.25269361402002	24.47539443217717	25.594643690030093	25.677268263772717
34	25.15789497555699	24.56486752696593	25.66300771124666	24.614229786230425
35	25.745650372825185	24.44718763417776	25.667562696920033	24.13959929607702
36	24.482543301562725	25.174400672504717	25.19526525231022	25.14779077362233
37	25.48357054520935	25.028045126195508	25.04066921265009	24.447715115945055
38	24.534800301477375	24.65749282403715	25.519817904036756	25.28788897044872
39	25.272703357709702	24.604381698524264	24.900556821325818	25.22235812244022
40	25.444220857856887	25.010318411826336	25.32433073949374	24.22112999082304
41	24.32147630307392	25.242956234616802	25.909007069467037	24.52656039284224
42	25.86156327907318	25.44754423283639	25.08114833780084	23.609744150289593
43	24.772771253371392	24.521504335941753	25.37160895489895	25.33411545578791
44	24.44644007220544	25.123592855652614	24.984125688264804	25.445841383877145
45	24.64808322334753	25.321302171444813	25.121967075314956	24.908647529892704
46	24.456008212223697	24.98930385076491	25.09377083481366	25.46091710219774
47	24.825325216741867	24.80748544660277	25.57467115483031	24.792518181825056
48	25.551425705406878	25.53388833076698	24.461235976714597	24.45344998711154
49	24.542314765890573	25.622209270946485	24.705818322829952	25.129657640332994
50	24.654375964266304	25.71243263836867	24.654073597566555	24.979117799798473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	54.0
2	48.0
3	144.0
4	240.0
5	282.5
6	325.0
7	323.5
8	322.0
9	416.0
10	510.0
11	811.0
12	1112.0
13	1882.5
14	2653.0
15	3509.5
16	4366.0
17	4580.0
18	4794.0
19	4837.5
20	4881.0
21	5387.5
22	5894.0
23	6536.0
24	7178.0
25	7894.0
26	8610.0
27	10285.5
28	11961.0
29	13956.0
30	15951.0
31	18179.0
32	20407.0
33	23568.0
34	26729.0
35	30890.5
36	35052.0
37	41387.0
38	47722.0
39	51905.0
40	56088.0
41	61725.0
42	67362.0
43	68654.5
44	69947.0
45	78061.5
46	86176.0
47	92328.5
48	98481.0
49	101389.5
50	104298.0
51	100357.0
52	96416.0
53	95264.5
54	94113.0
55	94712.0
56	95311.0
57	92825.0
58	90339.0
59	82138.5
60	73938.0
61	65212.0
62	56486.0
63	49395.0
64	42304.0
65	35859.0
66	29414.0
67	24932.0
68	20450.0
69	18754.0
70	17058.0
71	13174.5
72	9291.0
73	7932.0
74	6573.0
75	4986.5
76	3400.0
77	2979.0
78	2558.0
79	2139.0
80	1720.0
81	1376.0
82	1032.0
83	877.0
84	722.0
85	525.0
86	328.0
87	222.5
88	117.0
89	95.5
90	74.0
91	57.5
92	41.0
93	25.5
94	10.0
95	20.0
96	30.0
97	21.0
98	12.0
99	11.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008239386687267652
2	6.04725628423314E-4
3	3.779535177645712E-4
4	0.0
5	0.0
6	3.02362814211657E-4
7	0.0
8	0.0
9	0.0010582698497407996
10	0.0014362233675053707
11	7.559070355291425E-5
12	7.559070355291425E-5
13	7.559070355291425E-4
14	2.2677211065874275E-4
15	0.004686623620280684
16	0.0014362233675053707
17	0.0
18	1.511814071058285E-4
19	0.0
20	3.779535177645712E-4
21	0.0
22	2.2677211065874275E-4
23	0.001511814071058285
24	1.511814071058285E-4
25	0.0016629954781641136
26	0.00551812135936274
27	0.004837805027386512
28	0.00445985150962194
29	0.0021921304030345133
30	0.0035527630669869694
31	0.0067275726162093685
32	0.002721265327904913
33	0.004762214323833598
34	0.003099218845669484
35	0.0031748095492223987
36	0.007407888948185596
37	0.0034771723634340556
38	0.007181116837526854
39	0.003628353770539884
40	0.002721265327904913
41	0.0031748095492223987
42	0.002116539699481599
43	0.0013606326639524565
44	0.0016629954781641136
45	0.001209451256846628
46	6.04725628423314E-4
47	0.002040948995928685
48	0.0018897675888228562
49	5.291349248703998E-4
50	0.0012850419603995422
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1322914.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.421937872393556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75778824602327	40.23792006655371
2	13.661755029561466	14.323513467750626
3	4.213614215524894	6.626574678734408
4	1.806022320005031	3.787007594218392
5	0.9514802627141778	2.4939219609405665
6	0.5653550457038183	1.778220424903787
7	0.36734962032782437	1.3480025281990797
8	0.26734283109779355	1.121170342595067
9	0.2008680207390682	0.9476901813360684
>10	1.0293119846207288	9.614756343016486
>50	0.08431514508378034	3.0820799117979374
>100	0.0816068483568069	8.951856894923365
>500	0.01029143458392024	3.730963305043574
>1k	0.002898995657442321	1.9563222999868
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1605	0.12132307920242737	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1555	0.11754354402478166	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1554	0.11746795332122874	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1464	0.11066479000146646	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1438	0.10869943170909069	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1434	0.10839706889487902	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1401	0.10590257567763285	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1395	0.10544903145631537	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1385	0.10469312442078624	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1351	0.10212304049998715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	7.559070355291425E-5
11	0.0	0.0	0.0	0.0	7.559070355291425E-5
12	0.0	0.0	0.0	0.0	1.511814071058285E-4
13	0.0	0.0	0.0	0.0	1.511814071058285E-4
14	0.0	0.0	0.0	0.0	1.511814071058285E-4
15	0.0	0.0	0.0	0.0	1.511814071058285E-4
16	0.0	0.0	0.0	0.0	1.511814071058285E-4
17	7.559070355291425E-5	0.0	0.0	0.0	1.511814071058285E-4
18	7.559070355291425E-5	0.0	0.0	0.0	1.511814071058285E-4
19	7.559070355291425E-5	0.0	0.0	0.0	1.511814071058285E-4
20	7.559070355291425E-5	0.0	0.0	7.559070355291425E-5	1.511814071058285E-4
21	7.559070355291425E-5	0.0	0.0	1.511814071058285E-4	1.511814071058285E-4
22	7.559070355291425E-5	0.0	0.0	3.02362814211657E-4	1.511814071058285E-4
23	7.559070355291425E-5	0.0	0.0	9.826791461878851E-4	1.511814071058285E-4
24	7.559070355291425E-5	0.0	0.0	0.0021921304030345133	1.511814071058285E-4
25	7.559070355291425E-5	0.0	0.0	0.00302362814211657	1.511814071058285E-4
26	7.559070355291425E-5	0.0	0.0	0.0039307165847515405	2.2677211065874275E-4
27	7.559070355291425E-5	0.0	0.0	0.00551812135936274	2.2677211065874275E-4
28	7.559070355291425E-5	0.0	0.0	0.009826791461878853	2.2677211065874275E-4
29	7.559070355291425E-5	0.0	0.0	0.017461452520723193	2.2677211065874275E-4
30	7.559070355291425E-5	0.0	0.0	0.03333550026683518	2.2677211065874275E-4
31	7.559070355291425E-5	0.0	0.0	0.06357178168800089	2.2677211065874275E-4
32	7.559070355291425E-5	0.0	0.0	0.0965293284370715	2.2677211065874275E-4
33	7.559070355291425E-5	0.0	0.0	0.1263120656369197	2.2677211065874275E-4
34	7.559070355291425E-5	0.0	0.0	0.1601011101250724	2.2677211065874275E-4
35	7.559070355291425E-5	0.0	0.0	0.20122245285785773	2.2677211065874275E-4
36	7.559070355291425E-5	0.0	0.0	0.26086351796110707	2.2677211065874275E-4
37	7.559070355291425E-5	0.0	0.0	0.34507156171905357	2.2677211065874275E-4
38	7.559070355291425E-5	0.0	0.0	0.4625395150402823	2.2677211065874275E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACGT	25	0.0023531797	35.199066	17
CTACTAG	65	4.9346447E-4	20.306385	1
GTATCAA	3440	0.0	19.760355	1
CTAGCGG	440	0.0	19.500217	29
GTGTACG	70	8.127398E-4	18.855928	1
CGTATAA	60	0.0074136024	18.332844	44
CGTAACA	60	0.007416836	18.331457	2
CGCAATA	470	0.0	18.256212	36
TAGTACT	85	1.4324971E-4	18.11511	4
ATACGAA	475	0.0	18.063358	40
CCTAATA	100	2.4854819E-5	17.598202	2
ACCGTCG	300	0.0	17.597536	8
GTAAACG	290	0.0	17.450449	27
AACGCAG	3870	0.0	17.392914	6
TAGCGGC	495	0.0	17.334183	30
TCTAGCG	500	0.0	17.16149	28
CGAATGC	490	0.0	17.06077	43
TACCGTC	300	0.0	16.864305	7
TACGAAT	510	0.0	16.823082	41
CGTCGTA	340	0.0	16.822447	10
>>END_MODULE