FastQCFastQC Report
Thu 2 Feb 2017
SRR4064212_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064212_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516226
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61970.1762400937823678No Hit
TATCAACGCAGAGTACTTTTTTTTT38230.1087245245328372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.016.0358091
GTATCAA34500.013.8491481
TACCGTA751.4791904E-512.6672087
ACGCGCG1501.4551915E-1112.03384713
CGCGTAA2000.011.87550610
ACCGCTT1302.6175258E-911.6929748
TCGCGTA2050.011.585869
GCGTAAC2150.011.4891911
ACGAACG3150.011.46129615
ATAACGA3250.011.40064912
CGTTATT2250.011.3964322
GTATTAG4550.011.2734411
CGAACGA3300.011.22823116
AACGAAC3300.011.22807114
GATAACG3400.011.17726511
TACGCGT600.00588376511.0819145
ACTCTAA4050.011.02516310
CGCGCTA1658.0035534E-1110.94032816
TAACGAA3300.010.9398613
CGGTCCA4550.010.64880610
Produced by FastQC (version 0.11.5)