##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064212_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3516226
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25055101691416	32.0	32.0	32.0	32.0	32.0
2	30.830184692337752	32.0	32.0	32.0	32.0	32.0
3	30.846534608412544	32.0	32.0	32.0	32.0	32.0
4	30.882425646133097	32.0	32.0	32.0	32.0	32.0
5	30.775531493140658	32.0	32.0	32.0	32.0	32.0
6	34.429043810039516	36.0	36.0	36.0	32.0	36.0
7	34.37680399382747	36.0	36.0	36.0	32.0	36.0
8	34.33817792144191	36.0	36.0	36.0	32.0	36.0
9	34.49904556760572	36.0	36.0	36.0	32.0	36.0
10	34.17957747880825	36.0	36.0	36.0	32.0	36.0
11	34.459459943701	36.0	36.0	36.0	32.0	36.0
12	34.27110828484859	36.0	36.0	36.0	32.0	36.0
13	34.35318435163155	36.0	36.0	36.0	32.0	36.0
14	34.24855967733587	36.0	36.0	36.0	32.0	36.0
15	34.18008711612962	36.0	36.0	36.0	32.0	36.0
16	34.18094968867189	36.0	36.0	36.0	32.0	36.0
17	34.10728633483741	36.0	36.0	36.0	32.0	36.0
18	34.10342423951134	36.0	36.0	36.0	32.0	36.0
19	34.12763855338081	36.0	36.0	36.0	32.0	36.0
20	34.10923956537492	36.0	36.0	36.0	32.0	36.0
21	34.10177161536261	36.0	36.0	36.0	32.0	36.0
22	34.08286924674353	36.0	36.0	36.0	32.0	36.0
23	34.023273532474875	36.0	36.0	36.0	32.0	36.0
24	34.00581305069697	36.0	36.0	36.0	32.0	36.0
25	33.597128569096526	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	30.0
5	42.0
6	128.0
7	51.0
8	199.0
9	224.0
10	303.0
11	94.0
12	228.0
13	181.0
14	287.0
15	506.0
16	906.0
17	1158.0
18	1566.0
19	2057.0
20	3178.0
21	4944.0
22	7505.0
23	12114.0
24	18076.0
25	27347.0
26	39413.0
27	52222.0
28	71684.0
29	96933.0
30	128482.0
31	175421.0
32	246512.0
33	349208.0
34	735904.0
35	1539314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28782912050364	16.861172073841118	11.497147285639583	26.35385152001566
2	17.10684500760373	18.92463020155931	36.85274074192052	27.115784048916435
3	18.71115629910343	22.578682801623923	28.630287172851183	30.079873726421468
4	13.025718909356701	15.03013290331021	35.074130639431864	36.87001754790122
5	15.055241508280181	35.831139742741954	33.59599536395116	15.517623385026699
6	35.33682255133494	34.78323320864797	16.16664054635909	13.713303693658
7	31.0471813613938	30.091146070381765	20.31141905914908	18.55025350907535
8	28.530542793652042	32.75006479339088	18.825901083322734	19.89349132963435
9	27.228761602939123	14.202882385896903	18.124272482237256	40.44408352892672
10	15.788561604036614	26.421945721315087	31.513268609887675	26.27622406476063
11	37.315576720937734	21.00452034439527	22.16345970530974	19.516443229357254
12	24.691266680250287	23.18522064088131	28.459469396443293	23.66404328242511
13	29.40278060768594	19.22899831874998	25.065280764200416	26.302940309363663
14	23.720765174590067	19.03183825912792	24.76452915649402	32.482867409788
15	25.359189065058395	26.83305228039882	21.897457319887565	25.910301334655223
16	26.0882494979324	25.630191956730407	23.08361286055739	25.197945684779803
17	24.514552677028377	25.857114928257523	24.70319664589942	24.92513574881468
18	25.11412146183623	24.984794715135354	25.33560489995819	24.565478923070224
19	25.68178389993312	24.833634539581347	25.030324591237036	24.454256969248505
20	25.91267540612764	24.25762539743258	24.544718938923673	25.284980257516104
21	26.585955685994477	24.14763517946088	24.39413400531135	24.872275129233294
22	26.0719635443453	23.883821650539954	24.671975099678985	25.37223970543576
23	24.896630144121502	23.984453606310776	25.115053844544597	26.00386240502312
24	25.050668113256364	24.547473481956004	24.827617416112645	25.57424098867499
25	25.20159941847608	24.201777871637248	24.887242932813667	25.70937977707301
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	127.0
2	397.0
3	667.0
4	667.0
5	667.0
6	1733.5
7	2800.0
8	2800.0
9	2800.0
10	3643.5
11	4487.0
12	4487.0
13	4487.0
14	6652.5
15	8818.0
16	8818.0
17	8818.0
18	14894.5
19	20971.0
20	20971.0
21	20971.0
22	31716.0
23	42461.0
24	42461.0
25	42461.0
26	62623.5
27	82786.0
28	82786.0
29	82786.0
30	106390.0
31	129994.0
32	129994.0
33	129994.0
34	164964.0
35	199934.0
36	199934.0
37	199934.0
38	238682.0
39	277430.0
40	277430.0
41	277430.0
42	320802.0
43	364174.0
44	364174.0
45	364174.0
46	408755.5
47	453337.0
48	453337.0
49	453337.0
50	469961.0
51	486585.0
52	486585.0
53	486585.0
54	463234.0
55	439883.0
56	439883.0
57	439883.0
58	406273.0
59	372663.0
60	372663.0
61	372663.0
62	324301.5
63	275940.0
64	275940.0
65	275940.0
66	225244.5
67	174549.0
68	174549.0
69	174549.0
70	132184.0
71	89819.0
72	89819.0
73	89819.0
74	67750.5
75	45682.0
76	45682.0
77	45682.0
78	35437.5
79	25193.0
80	25193.0
81	25193.0
82	17186.0
83	9179.0
84	9179.0
85	9179.0
86	6809.0
87	4439.0
88	4439.0
89	4439.0
90	3163.5
91	1888.0
92	1888.0
93	1888.0
94	1305.5
95	723.0
96	723.0
97	723.0
98	1210.0
99	1697.0
100	1697.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03776776578069783
2	0.005375081123909555
3	0.0014504187159755943
4	0.003867783242601585
5	0.008560314382522626
6	0.012598735121121338
7	0.021898478652964855
8	0.03432657627808906
9	0.04854636761118313
10	0.05665164867104674
11	0.05380769040442793
12	0.062282686038951984
13	0.06182765271629298
14	0.06566699637622837
15	0.058585540292347534
16	0.0641881380775866
17	0.06253864228294768
18	0.07522269615206759
19	0.0732603649481006
20	0.07783913775735689
21	0.0744832670027467
22	0.07670155445070938
23	0.0816500418346261
24	0.07607588363205323
25	0.07675843361604175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3516226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.163436016849566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.08287033637166	33.00673538364434
2	13.887044210840413	12.543732653589043
3	4.895002122058956	6.632253454258576
4	2.3736753931337615	4.288133469702675
5	1.3321328313204357	3.0081847946642575
6	0.860080046762929	2.3306502096807917
7	0.608380619539979	1.9233591429139596
8	0.43863304555474747	1.5848140390230157
9	0.33464343731745555	1.3602282722770964
>10	1.9713006979994514	16.317918417368094
>50	0.12985411869366378	4.034919094412007
>100	0.07206231925189752	6.441369088588495
>500	0.00918813746963774	2.841470948131344
>1k	0.005069317219839937	3.508882354637108
>5k	6.336646524799923E-5	0.1773486771092347
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6197	0.1762400937823678	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3823	0.1087245245328372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8439582666188122E-5
2	0.0	0.0	0.0	0.0	2.8439582666188122E-5
3	0.0	0.0	0.0	0.0	2.8439582666188122E-5
4	0.0	0.0	0.0	0.0	2.8439582666188122E-5
5	0.0	0.0	0.0	0.0	5.6879165332376244E-5
6	0.0	0.0	0.0	0.0	5.6879165332376244E-5
7	0.0	0.0	0.0	2.8439582666188122E-5	5.6879165332376244E-5
8	0.0	0.0	0.0	1.1375833066475249E-4	5.6879165332376244E-5
9	0.0	0.0	0.0	1.7063749599712874E-4	5.6879165332376244E-5
10	0.0	0.0	0.0	2.559562439956931E-4	5.6879165332376244E-5
11	0.0	0.0	0.0	2.559562439956931E-4	5.6879165332376244E-5
12	0.0	0.0	0.0	2.559562439956931E-4	1.7063749599712874E-4
13	0.0	0.0	0.0	2.559562439956931E-4	2.2751666132950497E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	930	0.0	16.035809	1
GTATCAA	3450	0.0	13.849148	1
TACCGTA	75	1.4791904E-5	12.667208	7
ACGCGCG	150	1.4551915E-11	12.033847	13
CGCGTAA	200	0.0	11.875506	10
ACCGCTT	130	2.6175258E-9	11.692974	8
TCGCGTA	205	0.0	11.58586	9
GCGTAAC	215	0.0	11.48919	11
ACGAACG	315	0.0	11.461296	15
ATAACGA	325	0.0	11.400649	12
CGTTATT	225	0.0	11.396432	2
GTATTAG	455	0.0	11.273441	1
CGAACGA	330	0.0	11.228231	16
AACGAAC	330	0.0	11.228071	14
GATAACG	340	0.0	11.177265	11
TACGCGT	60	0.005883765	11.081914	5
ACTCTAA	405	0.0	11.025163	10
CGCGCTA	165	8.0035534E-11	10.940328	16
TAACGAA	330	0.0	10.93986	13
CGGTCCA	455	0.0	10.648806	10
>>END_MODULE