FastQCFastQC Report
Thu 2 Feb 2017
SRR4064212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516226
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.029.8063951
GTATCAA38050.029.024911
TCAACGC58000.018.774494
TATCAAC59750.018.4081752
CAACGCA59350.018.3845045
ATCAACG60100.018.0816173
AACGCAG60950.017.8297066
ACCGTCG7450.016.8312248
TACCGTC8200.016.3648917
ATACCGT8450.016.1408336
CGCAATA7500.015.54840536
CGTCGTA7900.015.31620810
CGACGGT8200.015.2917847
ACGCAGA71950.015.1651967
TCTAGCG7650.014.955070528
GTCCTAA3450.014.6666381
AGAGTAC74900.014.59768711
CGTATAT6050.014.5452210
ATTATAC6050.014.5441863
TAGCGTA5750.014.5382757
Produced by FastQC (version 0.11.5)