FastQCFastQC Report
Thu 2 Feb 2017
SRR4064211_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064211_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2223778
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55990.2517787297113291No Hit
TATCAACGCAGAGTACTTTTTTTTT33800.15199358928813936No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24210.10886878096644538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA604.086557E-412.669126516
CGTTATT1301.9826984E-1012.4193422
GTATCAA32200.012.0366261
CGAGCCG3000.012.035415
GCGTTAT1501.4551915E-1112.0326931
ACGCGCG658.021818E-411.69313
TCGAACG1157.099152E-811.562783
CGTCTTA1001.9266354E-611.401957515
CGCGCGT2000.011.3993937
CGGTCCA3000.011.39939310
AAGACGG3500.011.3991375
CAAGACG3350.011.3416594
AATTCCG1354.7366484E-911.2584065
CGAACGT1201.278604E-711.0817454
GGTTCTA3650.010.671864513
CGCAAGA3300.010.6483132
GGGCCGT1252.2442873E-710.6389546
GTTTTCG2350.010.51244415
AGGCCCG3450.010.4632110
GGCTTAT1002.401059E-510.4494441
Produced by FastQC (version 0.11.5)