##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064211_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2223778
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17310900638463	32.0	32.0	32.0	32.0	32.0
2	30.759693188798522	32.0	32.0	32.0	32.0	32.0
3	30.781388250086113	32.0	32.0	32.0	32.0	32.0
4	30.80672261349829	32.0	32.0	32.0	32.0	32.0
5	30.732814156808818	32.0	32.0	32.0	32.0	32.0
6	34.37064086433088	36.0	36.0	36.0	32.0	36.0
7	34.29178542102674	36.0	36.0	36.0	32.0	36.0
8	34.25976738685247	36.0	36.0	36.0	32.0	36.0
9	34.38462697265644	36.0	36.0	36.0	32.0	36.0
10	34.08825656158124	36.0	36.0	36.0	32.0	36.0
11	34.38086400710862	36.0	36.0	36.0	32.0	36.0
12	34.19114497939992	36.0	36.0	36.0	32.0	36.0
13	34.26522431645605	36.0	36.0	36.0	32.0	36.0
14	34.17152431582649	36.0	36.0	36.0	32.0	36.0
15	34.116110960716405	36.0	36.0	36.0	32.0	36.0
16	34.103651533561354	36.0	36.0	36.0	32.0	36.0
17	34.04400349315444	36.0	36.0	36.0	32.0	36.0
18	34.028525779102054	36.0	36.0	36.0	32.0	36.0
19	34.05114494342511	36.0	36.0	36.0	32.0	36.0
20	34.02711646576232	36.0	36.0	36.0	32.0	36.0
21	34.018943887384445	36.0	36.0	36.0	32.0	36.0
22	33.997724593012435	36.0	36.0	36.0	32.0	36.0
23	33.94260847980329	36.0	36.0	36.0	32.0	36.0
24	33.93655841545334	36.0	36.0	36.0	32.0	36.0
25	33.532093131598565	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	19.0
5	33.0
6	87.0
7	33.0
8	125.0
9	148.0
10	199.0
11	70.0
12	135.0
13	120.0
14	224.0
15	425.0
16	654.0
17	940.0
18	1264.0
19	1853.0
20	2896.0
21	4185.0
22	6668.0
23	10029.0
24	14715.0
25	20760.0
26	28437.0
27	36173.0
28	47783.0
29	62767.0
30	80972.0
31	107306.0
32	148157.0
33	206242.0
34	440344.0
35	1000013.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.874521690702466	17.502588920768677	11.557833026081745	26.06505636244711
2	16.391120337212403	20.220242188157002	37.799143117847606	25.589494356782993
3	18.487633030031308	24.45058923096433	28.913464037709343	28.148313701295024
4	12.641285939266494	16.0224348625388	36.53422566715924	34.80205353103546
5	14.416437791846315	36.6889747472408	33.95641851644143	14.938168944471453
6	32.98876665246963	36.067334422022526	17.445172386032816	13.498726539475028
7	29.44121795702175	30.938071232206344	21.41688976207954	18.203821048692372
8	27.435479074981522	33.107823304142535	20.028736446568338	19.4279611743076
9	27.309640422160452	14.582199182781727	18.84231610564269	39.26584428941513
10	15.392603724684697	26.964233487966094	32.33609452546041	25.307068261888798
11	36.038431635099236	21.770997559553376	23.275444715605182	18.915126089742206
12	24.508893839535165	23.858285325513492	29.33587895144892	22.296941883502424
13	28.828803290158994	20.208109755960376	25.54494383286424	25.418143121016385
14	23.35650546965311	19.92104764634601	25.924009372048456	30.79843751195242
15	24.638731704858785	27.328018473323112	22.896598705571513	25.13665111624659
16	25.094237485212467	26.363951760127186	24.08132795094836	24.46048280371199
17	23.572100632972052	26.174769041920502	25.650464615975803	24.602665709131635
18	24.028763898508206	25.734949648776762	26.413049690584227	23.823236762130804
19	24.969365488732397	25.42500181129501	26.022976670970273	23.582656029002322
20	25.25992045097582	24.98188590052502	25.465183575709737	24.29301007278943
21	26.0378603237919	24.736788577641295	25.19917485986658	24.026176238700224
22	25.24868815423526	24.82264205286986	25.449087783408185	24.47958200948669
23	24.2131162518154	24.772937058751257	25.762288356996365	25.25165833243698
24	24.313937093592756	25.403690174400996	25.68923563373316	24.59313709827309
25	24.419659070095438	25.06719997623868	25.75182293858194	24.761318015083937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	256.0
1	256.0
2	605.5
3	955.0
4	955.0
5	955.0
6	2392.5
7	3830.0
8	3830.0
9	3830.0
10	4425.0
11	5020.0
12	5020.0
13	5020.0
14	6423.0
15	7826.0
16	7826.0
17	7826.0
18	13055.5
19	18285.0
20	18285.0
21	18285.0
22	28237.5
23	38190.0
24	38190.0
25	38190.0
26	54624.5
27	71059.0
28	71059.0
29	71059.0
30	88110.0
31	105161.0
32	105161.0
33	105161.0
34	127123.5
35	149086.0
36	149086.0
37	149086.0
38	171098.5
39	193111.0
40	193111.0
41	193111.0
42	217269.5
43	241428.0
44	241428.0
45	241428.0
46	265829.5
47	290231.0
48	290231.0
49	290231.0
50	295318.0
51	300405.0
52	300405.0
53	300405.0
54	278908.5
55	257412.0
56	257412.0
57	257412.0
58	232292.0
59	207172.0
60	207172.0
61	207172.0
62	178124.5
63	149077.0
64	149077.0
65	149077.0
66	120513.5
67	91950.0
68	91950.0
69	91950.0
70	69163.5
71	46377.0
72	46377.0
73	46377.0
74	34850.5
75	23324.0
76	23324.0
77	23324.0
78	18156.0
79	12988.0
80	12988.0
81	12988.0
82	9067.0
83	5146.0
84	5146.0
85	5146.0
86	3951.0
87	2756.0
88	2756.0
89	2756.0
90	1989.5
91	1223.0
92	1223.0
93	1223.0
94	842.0
95	461.0
96	461.0
97	461.0
98	755.0
99	1049.0
100	1049.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037953428804493976
2	0.006070749868017401
3	0.0014389925613078284
4	0.004316977683923485
5	0.00984810534145045
6	0.013895271920128717
7	0.023113818016006992
8	0.036739278830890496
9	0.05018486557561052
10	0.05881882094345749
11	0.055491150645433134
12	0.06282101900459489
13	0.0626411449344314
14	0.06408013749573924
15	0.05980812832935662
16	0.06596881523245575
17	0.06318076714492185
18	0.07586188909144707
19	0.07244428175834099
20	0.0787848427316036
21	0.07239931324080011
22	0.07725591313521403
23	0.08170779637176014
24	0.07712100758259142
25	0.07550214095112012
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2223778.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.62117508358847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33326613527551	43.56988310579491
2	12.870791443684105	14.31777088707012
3	3.7253411679714312	6.216235600495168
4	1.5801935823505906	3.5156889563954032
5	0.8818557076833597	2.4524925357758987
6	0.536670391394608	1.791014268116247
7	0.3698435365295587	1.4399792469190893
8	0.2675019931602307	1.1903020157419262
9	0.20777379250819958	1.0400960241791777
>10	1.0872552578227757	10.645579994652069
>50	0.072242000662315	2.756410006431358
>100	0.058972247642357506	6.830524149536381
>500	0.0061789067837827736	2.3248916075571695
>1k	0.0020325351262442826	1.6559403597824314
>5k	8.130140504977128E-5	0.25319124155279593
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5599	0.2517787297113291	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3380	0.15199358928813936	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2421	0.10886878096644538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.993703508173927E-5
2	0.0	0.0	0.0	0.0	8.993703508173927E-5
3	0.0	0.0	0.0	0.0	8.993703508173927E-5
4	0.0	0.0	0.0	0.0	8.993703508173927E-5
5	0.0	0.0	0.0	0.0	8.993703508173927E-5
6	0.0	0.0	0.0	0.0	1.349055526226089E-4
7	0.0	0.0	0.0	0.0	1.349055526226089E-4
8	0.0	0.0	0.0	0.0	1.349055526226089E-4
9	0.0	0.0	0.0	0.0	1.349055526226089E-4
10	0.0	0.0	0.0	1.7987407016347855E-4	1.349055526226089E-4
11	0.0	0.0	0.0	1.7987407016347855E-4	1.7987407016347855E-4
12	0.0	0.0	0.0	1.7987407016347855E-4	2.2484258770434819E-4
13	0.0	0.0	0.0	1.7987407016347855E-4	2.698111052452178E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTA	60	4.086557E-4	12.6691265	16
CGTTATT	130	1.9826984E-10	12.419342	2
GTATCAA	3220	0.0	12.036626	1
CGAGCCG	300	0.0	12.0354	15
GCGTTAT	150	1.4551915E-11	12.032693	1
ACGCGCG	65	8.021818E-4	11.693	13
TCGAACG	115	7.099152E-8	11.56278	3
CGTCTTA	100	1.9266354E-6	11.4019575	15
CGCGCGT	200	0.0	11.399393	7
CGGTCCA	300	0.0	11.399393	10
AAGACGG	350	0.0	11.399137	5
CAAGACG	335	0.0	11.341659	4
AATTCCG	135	4.7366484E-9	11.258406	5
CGAACGT	120	1.278604E-7	11.081745	4
GGTTCTA	365	0.0	10.6718645	13
CGCAAGA	330	0.0	10.648313	2
GGGCCGT	125	2.2442873E-7	10.638954	6
GTTTTCG	235	0.0	10.512444	15
AGGCCCG	345	0.0	10.46321	10
GGCTTAT	100	2.401059E-5	10.449444	1
>>END_MODULE