FastQCFastQC Report
Thu 2 Feb 2017
SRR4064211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2223778
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35700.024.4661641
AACGCAG47100.018.2166166
GTATAGG2300.018.1747861
GTATTAT2550.018.1185171
ATAGCGT3950.017.8228386
TAGCGTA3950.017.2654887
TCAACGC50850.016.9164734
CAACGCA51400.016.692665
AATAGCG4250.016.5647565
GGTATCA16300.016.4670491
TATAGGG1906.184564E-1016.210212
TATCAAC53200.016.168862
ATCAACG53300.016.1388873
TAACGGC1805.2732503E-915.88893936
TATATCG850.002980932415.5294595
ACGATAG850.002980932415.52945935
AGCGTAT4400.015.4996998
CGTATAT4300.015.34888410
ACGCAGA56300.015.239497
GTATTAG5800.015.1731431
Produced by FastQC (version 0.11.5)