##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064211_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2223778 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32303089606966 32.0 32.0 32.0 32.0 32.0 2 31.40508180223026 32.0 32.0 32.0 32.0 32.0 3 31.471437796398742 32.0 32.0 32.0 32.0 32.0 4 31.550631402954792 32.0 32.0 32.0 32.0 32.0 5 31.51190901250035 32.0 32.0 32.0 32.0 32.0 6 35.08287338034642 36.0 36.0 36.0 36.0 36.0 7 35.09058188362327 36.0 36.0 36.0 36.0 36.0 8 35.041023879182184 36.0 36.0 36.0 36.0 36.0 9 35.137475053714894 36.0 36.0 36.0 36.0 36.0 10 35.00917402726351 36.0 36.0 36.0 36.0 36.0 11 35.141944924358455 36.0 36.0 36.0 36.0 36.0 12 35.05439256976191 36.0 36.0 36.0 36.0 36.0 13 35.09327279971292 36.0 36.0 36.0 36.0 36.0 14 35.041985306087206 36.0 36.0 36.0 36.0 36.0 15 35.01529334313047 36.0 36.0 36.0 36.0 36.0 16 35.028144446073306 36.0 36.0 36.0 36.0 36.0 17 34.994491356601245 36.0 36.0 36.0 36.0 36.0 18 34.990091636845044 36.0 36.0 36.0 36.0 36.0 19 34.99319356518502 36.0 36.0 36.0 36.0 36.0 20 34.97922679332199 36.0 36.0 36.0 36.0 36.0 21 34.97981318279073 36.0 36.0 36.0 36.0 36.0 22 34.96028560404861 36.0 36.0 36.0 36.0 36.0 23 34.91579285342332 36.0 36.0 36.0 32.0 36.0 24 34.89471655893709 36.0 36.0 36.0 32.0 36.0 25 34.8678573130951 36.0 36.0 36.0 32.0 36.0 26 34.8079731879711 36.0 36.0 36.0 32.0 36.0 27 34.801416328428466 36.0 36.0 36.0 32.0 36.0 28 34.782662657873225 36.0 36.0 36.0 32.0 36.0 29 34.75392867453496 36.0 36.0 36.0 32.0 36.0 30 34.741582118358934 36.0 36.0 36.0 32.0 36.0 31 34.72753215473847 36.0 36.0 36.0 32.0 36.0 32 34.687805167602164 36.0 36.0 36.0 32.0 36.0 33 34.67157557993649 36.0 36.0 36.0 32.0 36.0 34 34.66102146886964 36.0 36.0 36.0 32.0 36.0 35 34.62701942370147 36.0 36.0 36.0 32.0 36.0 36 34.60391235096309 36.0 36.0 36.0 32.0 36.0 37 34.591246967997705 36.0 36.0 36.0 32.0 36.0 38 34.56165498534476 36.0 36.0 36.0 32.0 36.0 39 34.56057664029413 36.0 36.0 36.0 32.0 36.0 40 34.53444003852903 36.0 36.0 36.0 32.0 36.0 41 34.51958154096317 36.0 36.0 36.0 32.0 36.0 42 34.45729609700249 36.0 36.0 36.0 32.0 36.0 43 34.44739402943999 36.0 36.0 36.0 32.0 36.0 44 34.397605336503915 36.0 36.0 36.0 32.0 36.0 45 34.35725508571449 36.0 36.0 36.0 32.0 36.0 46 34.33690188499032 36.0 36.0 36.0 32.0 36.0 47 34.3101730478492 36.0 36.0 36.0 32.0 36.0 48 34.2613502786699 36.0 36.0 36.0 32.0 36.0 49 34.24099707794573 36.0 36.0 36.0 32.0 36.0 50 33.65525920303196 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 14.0 20 35.0 21 104.0 22 299.0 23 897.0 24 2090.0 25 4652.0 26 9205.0 27 16487.0 28 26797.0 29 40257.0 30 56966.0 31 78929.0 32 111748.0 33 176179.0 34 364727.0 35 1334391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.984022676852604 18.398364086168804 12.065763951881395 26.5518492850972 2 15.651885721686293 20.925871630262925 37.23412196617986 26.18812068187092 3 17.83768591696552 24.92905082573552 29.279760767163943 27.95350249013502 4 12.310131676813063 16.613798679544452 36.55810067371833 34.517968969924155 5 14.039755766987533 37.07676755503472 33.772345980579 15.11113069739875 6 33.10054547008009 36.393601856307086 17.18208267941379 13.323769994199042 7 29.338849478926598 31.06789131094648 21.407291654956097 18.185967555170823 8 27.52662361081007 33.22292063326465 19.83723195390907 19.41322380201621 9 27.376944793453244 14.407637881460122 18.774749770433655 39.44066755465298 10 15.330625005059003 27.095079590495008 32.436802925498185 25.1374924789478 11 36.36064391319637 21.56132491642601 23.21198428979871 18.866046880578907 12 24.501196839785354 23.87168114731135 29.337122089349947 22.289999923553346 13 28.900425853393113 20.044968679314856 25.7002297899513 25.354375677340734 14 23.382232187263636 19.77763927449462 25.95209765021086 30.888030888030887 15 24.703002110475975 27.311708712043025 22.81441167727751 25.170877500203492 16 25.204024182509233 26.26515678878438 24.116135043592912 24.414683985113474 17 23.664682355882647 26.147169366726352 25.630616005734385 24.557532271656612 18 24.18819349979202 25.60609773920473 26.45042776360018 23.755280997403062 19 25.03563755015114 25.379152055645843 26.03915498759319 23.546055406609835 20 25.335647128022575 24.878044612647745 25.540569952566912 24.245738306762767 21 26.094196453063212 24.67431551171025 25.22877733298917 24.002710702237366 22 25.236321120616967 24.733032793335656 25.56917853649762 24.461467549549752 23 24.189728936819677 24.689422699011853 25.874081848687048 25.246766515481422 24 24.431168691006715 25.28480913044065 25.750999428897774 24.533022749654865 25 24.575017380644663 24.848295977313875 25.93659864606352 24.640087995977943 26 24.356703331395092 25.431596424458203 26.04418924989983 24.16751099424688 27 24.738975401155134 25.375184213638803 25.325670825461483 24.560169559744583 28 24.183475049602194 25.17448459588828 26.046547813276298 24.595492541233227 29 24.349884135540506 25.291594846291282 25.845621681516413 24.5128993366518 30 24.351082698056935 25.415927659436687 25.94936196544144 24.283627677064935 31 24.67339264208151 25.160886447222495 25.355838997525655 24.809881913170337 32 24.42732987184457 25.38316665962137 25.308066803795104 24.881436664738953 33 24.31614824181957 24.980853904611745 25.73086818267466 24.972129670894024 34 24.748675077516676 25.063418648522855 25.832492023483226 24.355414250477242 35 25.159563488797843 25.069894047055318 25.680761497722514 24.089780966424325 36 24.434489665683433 25.50918303606161 25.48557279128409 24.570754506970868 37 25.064824216870395 25.219565895851343 25.382627019938788 24.332982867339478 38 24.388313397247536 25.22942463727837 25.746197842603415 24.63606412287069 39 24.771596888069432 25.19031344156136 25.293115078472816 24.744974591896387 40 24.983282570207585 25.30076758522966 25.694700833330707 24.021249011232044 41 24.32838740576018 25.46531367553846 25.773176868438625 24.43312205026274 42 25.022237251916362 25.60706123810903 25.4026314156383 23.968070094336312 43 24.65496361777915 24.99353569711717 25.66807165010831 24.683429034995374 44 24.41267135992949 25.3837315711488 25.31812182547088 24.88547524345083 45 24.479639311324224 25.611872938725917 25.371917476186578 24.53657027376328 46 24.53296422858772 25.370824806105336 25.285159048260176 24.81105191704677 47 24.606575490063378 25.337055586009715 25.544273776241226 24.51209514768568 48 25.088690873687735 25.685839364852374 25.05536855785424 24.170101203605647 49 24.51096517262592 25.580771015681492 25.129779725223134 24.77848408646945 50 24.503935464055584 25.865957235358422 25.096717126407476 24.533390174178518 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 43.0 1 54.5 2 66.0 3 178.0 4 290.0 5 354.0 6 418.0 7 423.5 8 429.0 9 580.0 10 731.0 11 1083.0 12 1435.0 13 2603.0 14 3771.0 15 5027.5 16 6284.0 17 6544.5 18 6805.0 19 6846.5 20 6888.0 21 7903.5 22 8919.0 23 10412.5 24 11906.0 25 14497.0 26 17088.0 27 20765.5 28 24443.0 29 28430.5 30 32418.0 31 36474.5 32 40531.0 33 46039.0 34 51547.0 35 58826.5 36 66106.0 37 76557.5 38 87009.0 39 94985.0 40 102961.0 41 112353.5 42 121746.0 43 125507.0 44 129268.0 45 139694.5 46 150121.0 47 158041.0 48 165961.0 49 169679.0 50 173397.0 51 169957.0 52 166517.0 53 163816.5 54 161116.0 55 158079.5 56 155043.0 57 148544.5 58 142046.0 59 128367.0 60 114688.0 61 100117.0 62 85546.0 63 73527.0 64 61508.0 65 51674.0 66 41840.0 67 35216.5 68 28593.0 69 25364.5 70 22136.0 71 17189.5 72 12243.0 73 10419.5 74 8596.0 75 6641.5 76 4687.0 77 4096.0 78 3505.0 79 2865.0 80 2225.0 81 1738.0 82 1251.0 83 1078.5 84 906.0 85 630.0 86 354.0 87 243.5 88 133.0 89 100.5 90 68.0 91 57.5 92 47.0 93 46.0 94 45.0 95 45.5 96 46.0 97 37.0 98 28.0 99 29.0 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008499049815224362 2 6.295592455721749E-4 3 1.349055526226089E-4 4 0.0 5 0.0 6 3.597481403269571E-4 7 1.349055526226089E-4 8 0.0 9 8.993703508173926E-4 10 8.993703508173926E-4 11 0.0 12 2.2484258770434816E-4 13 3.597481403269571E-4 14 1.7987407016347855E-4 15 0.004901568411954791 16 9.893073858991321E-4 17 0.0 18 1.349055526226089E-4 19 0.0 20 6.295592455721749E-4 21 0.0 22 1.349055526226089E-4 23 7.194962806539142E-4 24 3.597481403269571E-4 25 0.0017987407016347853 26 0.005171379517200008 27 0.006160686903099141 28 0.003867292508514789 29 0.0026531425349113087 30 0.0033276702980243535 31 0.007194962806539141 32 0.003237733262942614 33 0.003912261026055658 34 0.002833016605074787 35 0.0024732684647478303 36 0.00750974242932523 37 0.003237733262942614 38 0.007734585017029578 39 0.003507544368187832 40 0.0022933943945843516 41 0.002833016605074787 42 0.0014389925613078284 43 0.0012141499736034803 44 0.0010342759034400017 45 0.0014389925613078284 46 8.54401833276523E-4 47 0.0015738981139304374 48 0.0019336462542573945 49 4.496851754086963E-4 50 0.0011242129385217409 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2223778.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.249358442793884 #Duplication Level Percentage of deduplicated Percentage of total 1 78.99821534177711 46.01595361784194 2 12.59424625597165 14.672135289618149 3 3.7134938819132683 6.489259086080643 4 1.5302081180537748 3.565345646423495 5 0.8003053602403462 2.3308636896164603 6 0.5064043700113573 1.7698637799473262 7 0.3390075981056761 1.3822882567821695 8 0.23773784464770453 1.1078461542641111 9 0.1806828868121245 0.9472196014558376 >10 0.9806572780477825 10.06895596599485 >50 0.06245613756359256 2.519608898841543 >100 0.049963273966457215 5.992229954583193 >500 0.005141518713988799 2.0338104944005693 >1k 0.0014801341752391267 1.1046195641497367 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 4.496851754086964E-5 0.0 11 0.0 0.0 0.0 4.496851754086964E-5 0.0 12 0.0 0.0 0.0 4.496851754086964E-5 1.349055526226089E-4 13 0.0 0.0 0.0 4.496851754086964E-5 3.1477962278608746E-4 14 0.0 0.0 0.0 8.993703508173927E-5 3.1477962278608746E-4 15 0.0 0.0 0.0 8.993703508173927E-5 4.4968517540869637E-4 16 0.0 0.0 0.0 8.993703508173927E-5 4.4968517540869637E-4 17 0.0 0.0 0.0 8.993703508173927E-5 4.4968517540869637E-4 18 0.0 0.0 0.0 8.993703508173927E-5 4.94653692949566E-4 19 0.0 0.0 0.0 8.993703508173927E-5 4.94653692949566E-4 20 0.0 0.0 0.0 8.993703508173927E-5 4.94653692949566E-4 21 0.0 0.0 0.0 1.349055526226089E-4 5.396222104904356E-4 22 0.0 0.0 0.0 1.349055526226089E-4 5.845907280313052E-4 23 0.0 0.0 0.0 1.349055526226089E-4 5.845907280313052E-4 24 0.0 0.0 0.0 1.7987407016347855E-4 5.845907280313052E-4 25 0.0 0.0 0.0 2.698111052452178E-4 5.845907280313052E-4 26 0.0 0.0 0.0 3.597481403269571E-4 6.295592455721749E-4 27 0.0 0.0 0.0 5.845907280313052E-4 6.295592455721749E-4 28 0.0 0.0 0.0 0.0012141499736034803 6.295592455721749E-4 29 0.0 0.0 0.0 0.0024282999472069605 6.295592455721749E-4 30 0.0 0.0 0.0 0.0047666628593321814 6.295592455721749E-4 31 0.0 0.0 0.0 0.014075145990292196 6.295592455721749E-4 32 0.0 0.0 0.0 0.02500249575272352 6.295592455721749E-4 33 0.0 0.0 0.0 0.03637953069056354 7.194962806539142E-4 34 0.0 0.0 0.0 0.04825121932135312 8.094333157356535E-4 35 0.0 0.0 0.0 0.06363045232033053 8.094333157356535E-4 36 0.0 0.0 0.0 0.08696911292404187 8.094333157356535E-4 37 0.0 0.0 0.0 0.12465273062329063 8.094333157356535E-4 38 0.0 0.0 0.0 0.17843507760217073 8.54401833276523E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3570 0.0 24.466164 1 AACGCAG 4710 0.0 18.216616 6 GTATAGG 230 0.0 18.174786 1 GTATTAT 255 0.0 18.118517 1 ATAGCGT 395 0.0 17.822838 6 TAGCGTA 395 0.0 17.265488 7 TCAACGC 5085 0.0 16.916473 4 CAACGCA 5140 0.0 16.69266 5 AATAGCG 425 0.0 16.564756 5 GGTATCA 1630 0.0 16.467049 1 TATAGGG 190 6.184564E-10 16.21021 2 TATCAAC 5320 0.0 16.16886 2 ATCAACG 5330 0.0 16.138887 3 TAACGGC 180 5.2732503E-9 15.888939 36 TATATCG 85 0.0029809324 15.529459 5 ACGATAG 85 0.0029809324 15.529459 35 AGCGTAT 440 0.0 15.499699 8 CGTATAT 430 0.0 15.348884 10 ACGCAGA 5630 0.0 15.23949 7 GTATTAG 580 0.0 15.173143 1 >>END_MODULE