FastQCFastQC Report
Thu 2 Feb 2017
SRR4064210_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064210_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115940
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55250.2611132640812121No Hit
TATCAACGCAGAGTACTTTTTTTTT34230.16177207293212473No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25060.11843436014253714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG500.0015011413.2984935
CGCGTAA905.4030716E-712.66762810
ACCGTCG2050.012.5134418
GCGTAAC951.0396925E-612.00062811
AAGACGG2400.011.8736545
CGCGTAT658.025185E-411.6923677
ACGCGTA658.026777E-411.6920896
CAAGACG2450.011.630514
CGTTATT1406.85759E-1011.530592
GTATCAA31550.011.500531
CGTCGTA2400.011.48003910
TAGACAG2400.011.4778665
GTGTACG752.0775846E-411.398171
CGAGCCG2450.011.246284515
ACTGTTC4500.011.1900048
TAATACC1701.0913936E-1111.1744134
TACCGTC2300.011.1522297
TACGCGT600.00588180211.0820785
CGCAAGA2700.010.9025632
CGGTTCT3150.010.85745312
Produced by FastQC (version 0.11.5)