##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064210_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2115940
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23689235044472	32.0	32.0	32.0	32.0	32.0
2	30.825585319054415	32.0	32.0	32.0	32.0	32.0
3	30.847055209504994	32.0	32.0	32.0	32.0	32.0
4	30.871678781061846	32.0	32.0	32.0	32.0	32.0
5	30.78464228664329	32.0	32.0	32.0	32.0	32.0
6	34.44084236793104	36.0	36.0	36.0	32.0	36.0
7	34.38697505600348	36.0	36.0	36.0	32.0	36.0
8	34.341884457971396	36.0	36.0	36.0	32.0	36.0
9	34.479130315604415	36.0	36.0	36.0	32.0	36.0
10	34.174348516498576	36.0	36.0	36.0	32.0	36.0
11	34.46561102866811	36.0	36.0	36.0	32.0	36.0
12	34.282099208862256	36.0	36.0	36.0	32.0	36.0
13	34.35751202775126	36.0	36.0	36.0	32.0	36.0
14	34.25341597587833	36.0	36.0	36.0	32.0	36.0
15	34.202064330746616	36.0	36.0	36.0	32.0	36.0
16	34.19276728073575	36.0	36.0	36.0	32.0	36.0
17	34.141205327183194	36.0	36.0	36.0	32.0	36.0
18	34.12933542539013	36.0	36.0	36.0	32.0	36.0
19	34.142090040360316	36.0	36.0	36.0	32.0	36.0
20	34.11844523001597	36.0	36.0	36.0	32.0	36.0
21	34.101470741136325	36.0	36.0	36.0	32.0	36.0
22	34.08005992608486	36.0	36.0	36.0	32.0	36.0
23	34.02957361739936	36.0	36.0	36.0	32.0	36.0
24	34.014590678374624	36.0	36.0	36.0	32.0	36.0
25	33.60935754321956	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	21.0
5	26.0
6	79.0
7	24.0
8	136.0
9	148.0
10	178.0
11	63.0
12	136.0
13	121.0
14	231.0
15	397.0
16	681.0
17	916.0
18	1124.0
19	1536.0
20	2261.0
21	3334.0
22	5239.0
23	8076.0
24	11987.0
25	17041.0
26	24002.0
27	31715.0
28	42759.0
29	57436.0
30	75140.0
31	100415.0
32	142345.0
33	199650.0
34	429277.0
35	959437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2774962082673	17.259664663320613	11.443042690560572	26.019796437851518
2	16.13213178582181	19.887542790596775	38.39070963741364	25.589615786167773
3	18.78857109369166	24.06094244618597	28.948134550909355	28.202351909213014
4	12.508164567431528	15.74265661554458	36.440957534796894	35.308221282227
5	14.177275616863092	36.88308373646162	34.175810411935274	14.763830234740007
6	33.37414010366495	35.91140739872437	17.30734871638286	13.407103781227814
7	29.533610910995517	30.70948699185608	21.27898445905446	18.477917638093942
8	27.41976736874602	33.79880701193697	19.796633215030496	18.984792404286512
9	27.44482881233474	14.237646533690928	18.692195083201252	39.625329570773076
10	15.438392407487534	27.09764672780966	32.524808307502425	24.939152557200376
11	36.46161826658136	21.180374783250556	23.433292241374826	18.924714708793253
12	24.682039975900583	23.462929176278532	29.665171330017298	22.189859517803587
13	29.447656051967506	20.03038941581952	25.456645190092274	25.065309342120695
14	23.18585267761713	19.84500296052196	25.7227267826031	31.246417579257805
15	24.554245502732574	28.010902867724703	22.542208653536505	24.89264297600622
16	24.800874722983895	26.01643510658322	24.7866396657741	24.39605050465879
17	23.386614709443098	26.20379653450363	25.913712545399513	24.49587621065375
18	23.908125460273734	25.426502591249488	27.097985035693828	23.567386912782943
19	25.17753926674332	24.942191843149644	25.84966994131922	24.030598948787816
20	25.365349750521116	24.986011303528937	25.575594064346586	24.073044881603362
21	25.907189599362823	24.51932809038505	25.04644492917858	24.527037381073548
22	25.222044693678125	25.109094916597847	25.401449427541394	24.267410962182637
23	24.103466935081055	24.961923123495843	25.871936821711294	25.06267311971181
24	24.480113587991912	25.15570106152935	25.98883417174792	24.37535117873082
25	24.431727617098943	25.001951038165142	25.953206300647174	24.613115044088733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	269.0
1	269.0
2	549.0
3	829.0
4	829.0
5	829.0
6	2328.0
7	3827.0
8	3827.0
9	3827.0
10	4189.5
11	4552.0
12	4552.0
13	4552.0
14	5618.0
15	6684.0
16	6684.0
17	6684.0
18	11409.5
19	16135.0
20	16135.0
21	16135.0
22	25272.0
23	34409.0
24	34409.0
25	34409.0
26	50910.5
27	67412.0
28	67412.0
29	67412.0
30	87048.5
31	106685.0
32	106685.0
33	106685.0
34	125097.5
35	143510.0
36	143510.0
37	143510.0
38	162584.0
39	181658.0
40	181658.0
41	181658.0
42	204691.5
43	227725.0
44	227725.0
45	227725.0
46	251268.0
47	274811.0
48	274811.0
49	274811.0
50	279361.0
51	283911.0
52	283911.0
53	283911.0
54	263453.0
55	242995.0
56	242995.0
57	242995.0
58	220424.0
59	197853.0
60	197853.0
61	197853.0
62	171293.5
63	144734.0
64	144734.0
65	144734.0
66	117168.5
67	89603.0
68	89603.0
69	89603.0
70	67490.0
71	45377.0
72	45377.0
73	45377.0
74	33829.0
75	22281.0
76	22281.0
77	22281.0
78	16923.0
79	11565.0
80	11565.0
81	11565.0
82	7986.5
83	4408.0
84	4408.0
85	4408.0
86	3351.5
87	2295.0
88	2295.0
89	2295.0
90	1635.0
91	975.0
92	975.0
93	975.0
94	675.0
95	375.0
96	375.0
97	375.0
98	718.5
99	1062.0
100	1062.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03837537926406231
2	0.005340416079851035
3	0.0019849334102101197
4	0.004253428736164542
5	0.00921576226168984
6	0.01313836876281936
7	0.023441118368195697
8	0.0353034585101657
9	0.05047402100248589
10	0.05789389113112848
11	0.05534183388942976
12	0.0645575961511196
13	0.06559732317551537
14	0.0684329423329584
15	0.05987882454133861
16	0.0681493804172141
17	0.06521924062118964
18	0.08076788566783556
19	0.07651445693167104
20	0.08190213333081278
21	0.07599459341947314
22	0.08157131109577775
23	0.08487953344612796
24	0.07712884108245036
25	0.0794445967276955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2115940.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.30007985445499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1193634511821	40.33348866821562
2	13.045510250933766	13.645624557318946
3	4.009486461645135	6.290893863581903
4	1.7196032360483653	3.5974154625323496
5	0.9223747035777666	2.4120135326423227
6	0.6211659443696099	1.9492217096039135
7	0.4084273477600104	1.4952548031814266
8	0.3123301383572596	1.3067912941630124
9	0.24812255837949113	1.1679146655245154
>10	1.424956777617999	13.68038001170577
>50	0.09396977730518297	3.3913210187545832
>100	0.06669573626417342	6.887219096189579
>500	0.00617688552286506	2.230338786980483
>1k	0.0017258944843299374	1.3496430996512745
>5k	9.083655180683881E-5	0.26247942995435575
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5525	0.2611132640812121	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3423	0.16177207293212473	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2506	0.11843436014253714	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4178095787215138E-4
2	0.0	0.0	0.0	0.0	1.4178095787215138E-4
3	0.0	0.0	0.0	0.0	1.4178095787215138E-4
4	0.0	0.0	0.0	0.0	1.4178095787215138E-4
5	0.0	0.0	0.0	0.0	1.4178095787215138E-4
6	0.0	0.0	0.0	0.0	1.4178095787215138E-4
7	0.0	0.0	0.0	0.0	1.4178095787215138E-4
8	0.0	0.0	0.0	0.0	1.4178095787215138E-4
9	0.0	0.0	0.0	4.726031929071713E-5	1.4178095787215138E-4
10	0.0	0.0	0.0	1.4178095787215138E-4	1.4178095787215138E-4
11	0.0	0.0	0.0	1.4178095787215138E-4	1.4178095787215138E-4
12	0.0	0.0	0.0	1.890412771628685E-4	2.8356191574430275E-4
13	0.0	0.0	0.0	1.890412771628685E-4	3.308222350350199E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	50	0.00150114	13.298493	5
CGCGTAA	90	5.4030716E-7	12.667628	10
ACCGTCG	205	0.0	12.513441	8
GCGTAAC	95	1.0396925E-6	12.000628	11
AAGACGG	240	0.0	11.873654	5
CGCGTAT	65	8.025185E-4	11.692367	7
ACGCGTA	65	8.026777E-4	11.692089	6
CAAGACG	245	0.0	11.63051	4
CGTTATT	140	6.85759E-10	11.53059	2
GTATCAA	3155	0.0	11.50053	1
CGTCGTA	240	0.0	11.480039	10
TAGACAG	240	0.0	11.477866	5
GTGTACG	75	2.0775846E-4	11.39817	1
CGAGCCG	245	0.0	11.2462845	15
ACTGTTC	450	0.0	11.190004	8
TAATACC	170	1.0913936E-11	11.174413	4
TACCGTC	230	0.0	11.152229	7
TACGCGT	60	0.005881802	11.082078	5
CGCAAGA	270	0.0	10.902563	2
CGGTTCT	315	0.0	10.857453	12
>>END_MODULE