##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2115940
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28353686777508	32.0	32.0	32.0	32.0	32.0
2	31.409502632399786	32.0	32.0	32.0	32.0	32.0
3	31.482822291747404	32.0	32.0	32.0	32.0	32.0
4	31.566318988251084	32.0	32.0	32.0	32.0	32.0
5	31.526532888456195	32.0	32.0	32.0	32.0	32.0
6	35.11246065578419	36.0	36.0	36.0	36.0	36.0
7	35.12490382525024	36.0	36.0	36.0	36.0	36.0
8	35.070815335028406	36.0	36.0	36.0	36.0	36.0
9	35.17152376721457	36.0	36.0	36.0	36.0	36.0
10	35.03330434700417	36.0	36.0	36.0	36.0	36.0
11	35.17470722232199	36.0	36.0	36.0	36.0	36.0
12	35.09365813775438	36.0	36.0	36.0	36.0	36.0
13	35.12232577483294	36.0	36.0	36.0	36.0	36.0
14	35.072322466610586	36.0	36.0	36.0	36.0	36.0
15	35.044392563116155	36.0	36.0	36.0	36.0	36.0
16	35.0622531829825	36.0	36.0	36.0	36.0	36.0
17	35.025994593419476	36.0	36.0	36.0	36.0	36.0
18	35.01895422365474	36.0	36.0	36.0	36.0	36.0
19	35.01967352571434	36.0	36.0	36.0	36.0	36.0
20	35.011165250432434	36.0	36.0	36.0	36.0	36.0
21	35.00511829257918	36.0	36.0	36.0	36.0	36.0
22	34.990773367864875	36.0	36.0	36.0	36.0	36.0
23	34.940532812839685	36.0	36.0	36.0	36.0	36.0
24	34.9206825335312	36.0	36.0	36.0	36.0	36.0
25	34.88971473671276	36.0	36.0	36.0	32.0	36.0
26	34.8372269535053	36.0	36.0	36.0	32.0	36.0
27	34.82468359216235	36.0	36.0	36.0	32.0	36.0
28	34.8032982976833	36.0	36.0	36.0	32.0	36.0
29	34.77513540081477	36.0	36.0	36.0	32.0	36.0
30	34.75828946000359	36.0	36.0	36.0	32.0	36.0
31	34.73991984649848	36.0	36.0	36.0	32.0	36.0
32	34.70623741693999	36.0	36.0	36.0	32.0	36.0
33	34.693540459559344	36.0	36.0	36.0	32.0	36.0
34	34.67954148038224	36.0	36.0	36.0	32.0	36.0
35	34.638860270139986	36.0	36.0	36.0	32.0	36.0
36	34.60931642674178	36.0	36.0	36.0	32.0	36.0
37	34.591474238399954	36.0	36.0	36.0	32.0	36.0
38	34.5579822679282	36.0	36.0	36.0	32.0	36.0
39	34.54329092507349	36.0	36.0	36.0	32.0	36.0
40	34.52910574023838	36.0	36.0	36.0	32.0	36.0
41	34.51525563106704	36.0	36.0	36.0	32.0	36.0
42	34.45657485561973	36.0	36.0	36.0	32.0	36.0
43	34.44782744312221	36.0	36.0	36.0	32.0	36.0
44	34.38933854457121	36.0	36.0	36.0	32.0	36.0
45	34.3575025756874	36.0	36.0	36.0	32.0	36.0
46	34.322178322636745	36.0	36.0	36.0	32.0	36.0
47	34.288092762554705	36.0	36.0	36.0	32.0	36.0
48	34.25074529523521	36.0	36.0	36.0	32.0	36.0
49	34.222157055493064	36.0	36.0	36.0	32.0	36.0
50	33.64777356635821	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	1.0
19	10.0
20	22.0
21	57.0
22	238.0
23	722.0
24	1815.0
25	4036.0
26	8162.0
27	14862.0
28	24727.0
29	37455.0
30	52698.0
31	74821.0
32	106576.0
33	170244.0
34	351250.0
35	1268241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.273363715167	18.122376211043147	11.953039554046862	26.651220519742992
2	15.440699001008538	20.59189936926199	37.907845896894706	26.059555732834767
3	18.13518493750505	24.680908781997143	29.123114128012677	28.060792152485124
4	12.165137007665622	16.326549902171138	36.56280423830543	34.9455088518578
5	13.792546102441468	37.29533918731155	33.93763528266397	14.974479427583013
6	33.446159575564124	36.163488722259345	17.13051138549428	13.259840316682247
7	29.500263949257466	30.751340895310207	21.301106790986687	18.447288364445633
8	27.42180780173351	34.00337438679736	19.618798264601075	18.95601954686806
9	27.399609720041003	14.104239438503496	18.578138934561235	39.91801190689427
10	15.366641760254755	27.163254204670213	32.64044357043576	24.829660464639275
11	36.62991389169825	21.121345595810844	23.402364906377308	18.846375606113593
12	24.53421388801851	23.483663754011815	29.744241209222004	22.23788114874767
13	29.592174792089153	19.93160457500741	25.49497124433642	24.98124938856702
14	23.126844633696216	19.711475068941155	25.84758038408552	31.31409991327711
15	24.618735147826975	28.022186807016748	22.520762321750293	24.838315723405984
16	24.88253369919364	25.991315386265875	24.712064599612653	24.41408631492784
17	23.470797848710266	26.19341758272919	25.865620008128776	24.47016456043177
18	24.065035962790954	25.339932143537354	27.100901397347844	23.49413049632385
19	25.223635830883673	24.935300622891006	25.902388536536954	23.938675009688364
20	25.46333076393395	24.888311469808297	25.68068210228226	23.967675663975495
21	25.871527548040113	24.463028252218873	25.156006313978658	24.509437885762356
22	25.285429507725667	24.929511033898034	25.533972231648054	24.251087226728245
23	24.23261839125982	24.74544278187028	25.910132145265745	25.111806681604154
24	24.594138466708035	25.11466583740207	26.0015095010187	24.289686194871194
25	24.622431599105443	24.85585384619747	26.08917779033871	24.432536764358375
26	24.069069494857835	25.467899274047184	26.346699183303084	24.116332047791893
27	24.418709617350544	25.335881084366445	25.796877193264372	24.44853210501864
28	24.026406134208496	25.294819346349872	25.94821425870752	24.73056026073411
29	24.296940870439755	25.065362380759737	25.72928915220786	24.90840759659265
30	24.16764420363899	25.42892683927402	25.901452930295076	24.501976026791915
31	24.83190707601969	24.82954389699564	25.510470300983933	24.828078726000737
32	24.262115177663752	25.53586476353327	25.39776612934861	24.80425392945438
33	24.02857099645911	25.136777052459248	26.080746668481535	24.753905282600105
34	24.565228384772112	25.135345513287373	26.134881876627276	24.16454422531324
35	24.88283856770976	25.20993589440631	25.933510532713516	23.973715005170416
36	24.12914654423532	25.488775393492197	25.766873775284786	24.615204286987698
37	24.753269340743998	25.209062938649353	25.489987570124818	24.547680150481835
38	24.400756029305352	25.42032743309712	25.694173006691113	24.484743530906407
39	24.887657761208704	24.930524572560703	25.407541912115	24.774275754115592
40	25.201923722221014	25.169738805934877	25.74821659645219	23.880120875391913
41	24.328419508409553	25.016978855476268	26.321455932721673	24.33314570339251
42	24.88175244576776	25.62899948012666	25.748901176804196	23.740346897301386
43	24.129960220406453	25.107459725347358	26.094269649592682	24.66831040465351
44	23.909431101642127	25.48151767983009	25.65581587909527	24.95323533943251
45	24.025832856785023	25.625475563705454	25.6764229102372	24.672268669272324
46	24.16890800743506	25.026690504985773	25.692972469198992	25.11142901838018
47	24.524268632733115	25.17968713077178	25.88799092584716	24.40805331064795
48	24.524034126247454	25.483149550925653	25.370715550959684	24.62210077186721
49	24.358285446656392	25.419330385224864	25.451183945805468	24.77120022231328
50	24.078598880480627	25.955145497819377	25.29202592929371	24.674229692406286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	113.0
1	94.0
2	75.0
3	254.5
4	434.0
5	478.5
6	523.0
7	497.0
8	471.0
9	652.5
10	834.0
11	1209.0
12	1584.0
13	2742.0
14	3900.0
15	4956.5
16	6013.0
17	6180.0
18	6347.0
19	6298.5
20	6250.0
21	7145.5
22	8041.0
23	9425.0
24	10809.0
25	13157.5
26	15506.0
27	19729.0
28	23952.0
29	28196.0
30	32440.0
31	36998.5
32	41557.0
33	49118.0
34	56679.0
35	65511.0
36	74343.0
37	82011.0
38	89679.0
39	94304.0
40	98929.0
41	104352.5
42	109776.0
43	113407.0
44	117038.0
45	126177.0
46	135316.0
47	141963.5
48	148611.0
49	152622.5
50	156634.0
51	155728.5
52	154823.0
53	153522.5
54	152222.0
55	149231.0
56	146240.0
57	139885.0
58	133530.0
59	122549.0
60	111568.0
61	98425.5
62	85283.0
63	73474.5
64	61666.0
65	51879.5
66	42093.0
67	35705.0
68	29317.0
69	25238.0
70	21159.0
71	16454.5
72	11750.0
73	9929.0
74	8108.0
75	6324.0
76	4540.0
77	3836.5
78	3133.0
79	2611.5
80	2090.0
81	1636.5
82	1183.0
83	982.5
84	782.0
85	524.5
86	267.0
87	195.5
88	124.0
89	97.5
90	71.0
91	58.0
92	45.0
93	38.0
94	31.0
95	31.0
96	31.0
97	21.0
98	11.0
99	15.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00954658449672486
2	2.835619157443028E-4
3	4.253428736164542E-4
4	0.0
5	0.0
6	9.452063858143426E-5
7	1.417809578721514E-4
8	0.0
9	0.001086987343686494
10	8.979460665236254E-4
11	0.0
12	4.726031929071713E-5
13	4.253428736164542E-4
14	2.3630159645358562E-4
15	0.004158908097583108
16	6.616444700700398E-4
17	0.0
18	1.417809578721514E-4
19	0.0
20	5.198635121978885E-4
21	0.0
22	2.835619157443028E-4
23	5.198635121978885E-4
24	4.253428736164542E-4
25	0.001512330217302948
26	0.004726031929071713
27	0.00411164777829239
28	0.003213701711768765
29	0.002079454048791554
30	0.003686304904675936
31	0.0069000066164447
32	0.002882879476733745
33	0.004158908097583108
34	0.0027883588381523105
35	0.0026465778802801593
36	0.006710965339281832
37	0.003308222350350199
38	0.007041787574316852
39	0.003969866820420239
40	0.0019849334102101197
41	0.00345000330822235
42	0.001890412771628685
43	0.001465069898012231
44	0.0012287683015586453
45	0.0014178095787215138
46	8.979460665236254E-4
47	0.001890412771628685
48	0.001512330217302948
49	3.308222350350199E-4
50	0.001512330217302948
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2115940.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.620195856582114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52697050277648	44.4621244989125
2	12.54192924202592	14.20252980205804
3	3.7060600330637525	6.295135347849623
4	1.6298327635466066	3.6912580114193334
5	0.8617740013172241	2.4396906369345843
6	0.512894550826636	1.7424113952946705
7	0.3914759843520378	1.5515812835012455
8	0.2773190579813432	1.2561487502133202
9	0.21036243465307225	1.0719686025831965
>10	1.2084217644715711	12.526797650232508
>50	0.0764727299410956	2.9651056022410827
>100	0.05161357213415927	5.696319227591907
>500	0.00403312792567948	1.5304642462402553
>1k	8.402349845165424E-4	0.5684649449277136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.452063858143426E-5
2	0.0	0.0	0.0	0.0	9.452063858143426E-5
3	0.0	0.0	0.0	0.0	9.452063858143426E-5
4	0.0	0.0	0.0	0.0	9.452063858143426E-5
5	0.0	4.726031929071713E-5	0.0	0.0	9.452063858143426E-5
6	0.0	4.726031929071713E-5	0.0	0.0	9.452063858143426E-5
7	0.0	4.726031929071713E-5	0.0	0.0	9.452063858143426E-5
8	0.0	4.726031929071713E-5	0.0	0.0	9.452063858143426E-5
9	0.0	4.726031929071713E-5	0.0	0.0	9.452063858143426E-5
10	0.0	4.726031929071713E-5	0.0	4.726031929071713E-5	9.452063858143426E-5
11	0.0	4.726031929071713E-5	0.0	4.726031929071713E-5	9.452063858143426E-5
12	0.0	4.726031929071713E-5	0.0	4.726031929071713E-5	2.8356191574430275E-4
13	0.0	4.726031929071713E-5	0.0	9.452063858143426E-5	3.308222350350199E-4
14	0.0	4.726031929071713E-5	0.0	9.452063858143426E-5	3.308222350350199E-4
15	0.0	4.726031929071713E-5	0.0	9.452063858143426E-5	5.198635121978885E-4
16	0.0	4.726031929071713E-5	0.0	2.3630159645358565E-4	5.671238314886055E-4
17	0.0	4.726031929071713E-5	0.0	2.3630159645358565E-4	5.671238314886055E-4
18	0.0	4.726031929071713E-5	0.0	2.3630159645358565E-4	5.671238314886055E-4
19	0.0	9.452063858143426E-5	0.0	2.3630159645358565E-4	5.671238314886055E-4
20	0.0	9.452063858143426E-5	0.0	3.308222350350199E-4	6.143841507793227E-4
21	0.0	9.452063858143426E-5	0.0	3.308222350350199E-4	6.616444700700398E-4
22	0.0	9.452063858143426E-5	0.0	4.2534287361645413E-4	7.089047893607569E-4
23	0.0	9.452063858143426E-5	0.0	9.924667051050596E-4	7.089047893607569E-4
24	0.0	9.452063858143426E-5	0.0	0.0013232889401400797	7.089047893607569E-4
25	0.0	9.452063858143426E-5	0.0	0.0015595905365936651	7.089047893607569E-4
26	0.0	9.452063858143426E-5	0.0	0.002268495325954422	7.56165108651474E-4
27	0.0	9.452063858143426E-5	0.0	0.003024660434605896	7.56165108651474E-4
28	0.0	9.452063858143426E-5	0.0	0.007089047893607569	7.56165108651474E-4
29	0.0	9.452063858143426E-5	0.0	0.016352070474588126	7.56165108651474E-4
30	0.0	9.452063858143426E-5	0.0	0.0322787980755598	7.56165108651474E-4
31	0.0	9.452063858143426E-5	0.0	0.0667788311577833	8.506857472329083E-4
32	0.0	9.452063858143426E-5	0.0	0.1056740739340435	9.452063858143426E-4
33	0.0	9.452063858143426E-5	0.0	0.1390398593532898	9.452063858143426E-4
34	0.0	9.452063858143426E-5	4.726031929071713E-5	0.1746741400984905	0.0011815079822679283
35	0.0	9.452063858143426E-5	4.726031929071713E-5	0.21952418310538105	0.0011815079822679283
36	0.0	9.452063858143426E-5	4.726031929071713E-5	0.2823804077620348	0.0011815079822679283
37	0.0	9.452063858143426E-5	4.726031929071713E-5	0.38105995444105223	0.0011815079822679283
38	0.0	9.452063858143426E-5	4.726031929071713E-5	0.5211395408187378	0.0012287683015586453
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3505	0.0	23.036692	1
CGGCTAA	105	7.75708E-8	20.951334	9
TAGGACG	1085	0.0	19.869976	4
AGGACGT	1140	0.0	18.332418	5
GGACGTG	1165	0.0	18.12785	6
TTAGGAC	755	0.0	18.065323	3
GTAGGAC	1180	0.0	17.897411	3
TAGTACG	75	0.001291942	17.598705	7
AACGCAG	4520	0.0	17.569918	6
GACGTGA	735	0.0	16.760672	7
TGTAGGA	1230	0.0	16.633316	2
GATATAC	650	0.0	16.585354	1
TATACTG	320	0.0	16.499176	5
TATCGAG	80	0.0019896787	16.499176	10
TCAACGC	4920	0.0	16.096756	4
TATCAAC	5015	0.0	15.923432	2
GTATTAG	365	0.0	15.671932	1
ATCAACG	5070	0.0	15.663913	3
CAACGCA	5050	0.0	15.638823	5
TAGGACC	985	0.0	15.633738	4
>>END_MODULE