##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064209_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1903945
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29710627145217	32.0	32.0	32.0	32.0	32.0
2	31.014511973822774	32.0	32.0	32.0	32.0	32.0
3	31.028828563850322	32.0	32.0	32.0	32.0	32.0
4	31.048511380318235	32.0	32.0	32.0	32.0	32.0
5	30.98472802523182	32.0	32.0	32.0	32.0	32.0
6	34.60397385428676	36.0	36.0	36.0	32.0	36.0
7	34.568010105333926	36.0	36.0	36.0	32.0	36.0
8	34.53940633789316	36.0	36.0	36.0	32.0	36.0
9	34.646882656799434	36.0	36.0	36.0	32.0	36.0
10	34.43068313422919	36.0	36.0	36.0	32.0	36.0
11	34.602468033477855	36.0	36.0	36.0	32.0	36.0
12	34.498650958930014	36.0	36.0	36.0	32.0	36.0
13	34.546663375255065	36.0	36.0	36.0	32.0	36.0
14	34.46154116846863	36.0	36.0	36.0	32.0	36.0
15	34.405529571494974	36.0	36.0	36.0	32.0	36.0
16	34.40034454776792	36.0	36.0	36.0	32.0	36.0
17	34.3510742169548	36.0	36.0	36.0	32.0	36.0
18	34.33709482154159	36.0	36.0	36.0	32.0	36.0
19	34.353315878347324	36.0	36.0	36.0	32.0	36.0
20	34.3318052779886	36.0	36.0	36.0	32.0	36.0
21	34.32103605933995	36.0	36.0	36.0	32.0	36.0
22	34.29971243917235	36.0	36.0	36.0	32.0	36.0
23	34.26825249678956	36.0	36.0	36.0	32.0	36.0
24	34.23184073069338	36.0	36.0	36.0	32.0	36.0
25	33.84842629382676	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	14.0
5	25.0
6	85.0
7	24.0
8	89.0
9	122.0
10	155.0
11	49.0
12	112.0
13	88.0
14	174.0
15	314.0
16	544.0
17	775.0
18	938.0
19	1261.0
20	1696.0
21	2471.0
22	3653.0
23	5272.0
24	7963.0
25	11511.0
26	17025.0
27	23246.0
28	32514.0
29	44568.0
30	60026.0
31	83345.0
32	120712.0
33	174751.0
34	392295.0
35	918122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.48633794794949	16.998331256160586	11.377197056789832	25.1381337391001
2	17.242730481553075	19.092203126313134	36.95578409950416	26.70928229262963
3	19.293488108987944	22.545205489525017	27.785139793383852	30.37616660810319
4	12.586658514896676	14.551697296207063	34.91305391460916	37.948590274287106
5	14.63892247714277	36.07171859685025	33.12315432399014	16.166204602016837
6	34.96179541082672	35.56073494850549	15.869271281474267	13.608198359193526
7	32.01296951313368	30.0855090591692	19.044244384544385	18.857277043152735
8	29.476181794586598	31.560337159468776	18.71830854935578	20.245172496588843
9	27.408908286663426	13.367684629458951	18.30388811412225	40.91951896975537
10	15.82083575507304	27.351277154577584	29.730748088971477	27.097139001377897
11	38.29535553670767	21.605461824090337	20.507957627982602	19.5912250112194
12	25.169146358767915	23.332159618244788	28.328454532554826	23.170239490432472
13	29.28067127257362	19.507046028587734	24.8641719931006	26.348110705738044
14	24.113059199461823	19.575344769593002	24.207397830474267	32.104198200470904
15	25.944455714137067	26.947744479047657	21.71268029610446	25.395119510710824
16	26.84436848373239	25.98628923162306	22.649203411084446	24.5201388735601
17	24.571054988206903	25.20832369844987	24.12755254339902	26.09306876994421
18	24.541787228227303	26.270672858340493	24.521498721456148	24.666041191976053
19	25.853911335973233	25.17005694319031	24.3549609533911	24.621070767445357
20	26.20366024353618	24.56323115648411	24.43976534663197	24.79334325334774
21	27.24992116051719	23.366708714390832	24.666666666666668	24.71670345842531
22	26.994788559534726	23.248753643146415	24.93344441261573	24.82301338470312
23	25.231921088222258	23.456132761518	25.301987564645916	26.009958585613823
24	25.664735934740563	25.51567361870322	23.327884534521907	25.491705912034313
25	25.459430707484394	24.52711382276203	24.824080245057743	25.18937522469583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79.0
1	79.0
2	223.5
3	368.0
4	368.0
5	368.0
6	1102.0
7	1836.0
8	1836.0
9	1836.0
10	2134.0
11	2432.0
12	2432.0
13	2432.0
14	2879.5
15	3327.0
16	3327.0
17	3327.0
18	5869.0
19	8411.0
20	8411.0
21	8411.0
22	13586.5
23	18762.0
24	18762.0
25	18762.0
26	31272.5
27	43783.0
28	43783.0
29	43783.0
30	56470.5
31	69158.0
32	69158.0
33	69158.0
34	83807.0
35	98456.0
36	98456.0
37	98456.0
38	122197.0
39	145938.0
40	145938.0
41	145938.0
42	169912.5
43	193887.0
44	193887.0
45	193887.0
46	221139.5
47	248392.0
48	248392.0
49	248392.0
50	258116.0
51	267840.0
52	267840.0
53	267840.0
54	255486.0
55	243132.0
56	243132.0
57	243132.0
58	226075.0
59	209018.0
60	209018.0
61	209018.0
62	183617.0
63	158216.0
64	158216.0
65	158216.0
66	128756.0
67	99296.0
68	99296.0
69	99296.0
70	72633.5
71	45971.0
72	45971.0
73	45971.0
74	35018.0
75	24065.0
76	24065.0
77	24065.0
78	18700.0
79	13335.0
80	13335.0
81	13335.0
82	8657.5
83	3980.0
84	3980.0
85	3980.0
86	3080.5
87	2181.0
88	2181.0
89	2181.0
90	1511.0
91	841.0
92	841.0
93	841.0
94	594.5
95	348.0
96	348.0
97	348.0
98	620.5
99	893.0
100	893.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03765865085388496
2	0.0055148651878074205
3	0.0014706307167486456
4	0.0033614416382826183
5	0.00898135187728637
6	0.012973063822746982
7	0.02121910034165903
8	0.03319423617804086
9	0.04532693959121718
10	0.05478099419888705
11	0.05204982286778242
12	0.06066351706588163
13	0.06150387747545229
14	0.06434009385775324
15	0.057932345734777
16	0.06339468839698627
17	0.05971811160511464
18	0.07300631058145063
19	0.07074784198072948
20	0.07321640068384327
21	0.07064279692953315
22	0.07353153583743229
23	0.07699802252691122
24	0.0727436979534598
25	0.0721134276462818
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1903945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.96005833932525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.92299136398294	25.473977817336202
2	15.86713156894453	11.729002169318784
3	6.118035055861176	6.783687977599984
4	2.9585288144853408	4.37389590327812
5	1.6654321980578444	3.077723560020431
6	0.9919100325389776	2.1996631602001577
7	0.6608245389782295	1.7096879458885155
8	0.4588470655311534	1.3567211448687702
9	0.3367060679759414	1.1200208324036047
>10	1.6643685452613546	11.224762356032306
>50	0.15121507740395337	3.951625803432864
>100	0.16810889957087002	13.105539062897343
>500	0.023363994090736296	6.049638965382292
>1k	0.01253677731698045	7.844053301340646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGCAG	4812	0.2527383931783744	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4563	0.23966028430443104	No Hit
TCCCTGGTCAGTGCCAGTTCTGAGT	3637	0.19102442560052943	No Hit
GTCCTTGACAGTCCTTGAAAATCCA	3504	0.18403892969597338	No Hit
GTTCTAAGTTGGTTGTTAAGCGTAC	3454	0.18141280341606505	No Hit
GTCATACACCGGCGCAGAAGCGTTG	3179	0.16696910887656943	No Hit
ATGTAGATAAGGGAAGTCGGCAAAA	2985	0.15677973891052524	No Hit
TATCAACGCAGAGTACATGGGCAGA	2920	0.15336577474664448	No Hit
GTACATGGGCAGAAATCACATTGCG	2887	0.151632531401905	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2873	0.15089721604353068	No Hit
GTCCCAATCAGTCCGGCCGTTTCCA	2751	0.14448946792055442	No Hit
GTGTATGACCGGGATCCACCTTGCG	2581	0.13556063856886624	No Hit
ACGCAGAGTACATGGGCAGAAATCA	2557	0.13430009795451023	No Hit
GTCTACCACCCTGAAATCGGATTAT	2318	0.1217472143365486	No Hit
GTGCCAGTTCTGAGTTAATCTTTCA	2222	0.11670505187912465	No Hit
GACTAATGTAGGTAAGGGAAGTCGG	2057	0.1080388351554273	No Hit
TATCTACATTGTTCTATCAACTAGA	2051	0.10772370000183829	No Hit
GTCAAGGACGCACCGGACTCGACGG	2044	0.10735604232265113	No Hit
GCCTCTAGTTGATAGAACAATGTAG	2010	0.10557027645231348	No Hit
CCCTTAGAGCCAATCCTTTTCCCGA	2007	0.10541270887551898	No Hit
ACTAGAGGCTGTTCACCTTGGAGAC	1971	0.10352189795398502	No Hit
TCGCAATGCTATGTTTTAATTAGAC	1942	0.10199874471163821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTT	55	5.906568E-7	17.274065	9
GGTATCA	1185	0.0	16.511272	1
CGCGTTC	65	3.3712931E-6	14.616133	10
TAGCGGT	40	0.00528341	14.248482	4
GACTCGA	355	0.0	13.649303	16
ACTCGAC	345	0.0	13.494153	17
GTATCAA	3890	0.0	13.331369	1
CTCGACG	350	0.0	13.30138	18
ACCGGAC	350	0.0	13.30103	12
CTATACG	50	0.0015021571	13.297185	1
TCGACGG	350	0.0	12.758467	19
CGTTCGG	75	1.47781775E-5	12.667648	12
GCGTTCG	75	1.4782172E-5	12.6673155	11
TCTATAG	75	1.4826175E-5	12.663653	3
CGACCAT	225	0.0	12.24507	10
CTTATGG	55	0.003074354	12.088033	3
ACAGTCC	615	0.0	12.049714	8
CGTTATT	150	1.4551915E-11	12.028574	2
TTTAAAC	120	1.0053554E-8	11.872175	3
CGCAAGA	280	0.0	11.870304	2
>>END_MODULE