FastQCFastQC Report
Thu 2 Feb 2017
SRR4064209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903945
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTCAGTGCCAGTTCTGAGTTAATCTTTCATTGGAGCCCGAGACA34730.18241073140243022No Hit
GTCATACACCGGCGCAGAAGCGTTGTTCGAAGGTCTTGGCTGAAGGCACC28910.15184262150429764No Hit
GTTCTAAGTTGGTTGTTAAGCGTACACCAGACGTCCGAAGACAGCCAAGG28860.15158000887630682No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA28000.14706307167486457No Hit
GTCCTTGACAGTCCTTGAAAATCCAAGGGAATGAATAATTTTTGCGCCTG27000.14181081911504798No Hit
GTCCCAATCAGTCCGGCCGTTTCCAACCAAGGCCCGATGCACCCAACCCT21690.11392135802242187No Hit
GTCTACCACCCTGAAATCGGATTATCCGGAGCTAGGGTTCAATGGCTGGT21490.11287090751045854No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA20890.1097195559745686No Hit
GACTAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGAAA20640.10840649283461444No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAAAACCACTT20630.10835397030901628No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAG19980.10494000614513549No Hit
GTCAAGGACGCACCGGACTCGACGGAGGTGCCGAGCTCTACCAGCCATTG19710.10352189795398502No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCGTG19510.1024714474420217No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG19480.10231387986522719No Hit
GTGTATGACCGGGATCCACCTTGCGGCATCACCCAGCCCGCCCGGCTCCA19440.10210378976283453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.029.8319321
GTATCAA39500.028.406421
ATCAACG61350.018.144033
ATACCGT3550.017.9708026
AACGCAG61950.017.932796
TCAACGC62350.017.8530274
CAACGCA63250.017.5989935
GTCATAC10350.017.2182141
CATACAC11550.017.1418743
TAATACG1053.7780366E-516.7609444
AACCGTA1602.132765E-816.4990547
TATCAAC69150.016.3837322
TACACCG10600.016.1877525
TAGCGGC4100.016.09917430
ATACACC10900.015.9440414
GTATAAT1401.586508E-615.7150351
ACGGACC7000.015.7133868
TACCGTC3800.015.6306837
AAGACGG7200.015.582445
GTATTAG3250.015.5699731
Produced by FastQC (version 0.11.5)