##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064209_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1903945
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35210260800601	32.0	32.0	32.0	32.0	32.0
2	31.370487067641136	32.0	32.0	32.0	32.0	32.0
3	31.461062163035173	32.0	32.0	32.0	32.0	32.0
4	31.54318533360995	32.0	32.0	32.0	32.0	32.0
5	31.477945003663446	32.0	32.0	32.0	32.0	32.0
6	35.08408015987857	36.0	36.0	36.0	36.0	36.0
7	35.105895916111024	36.0	36.0	36.0	36.0	36.0
8	35.04349600434887	36.0	36.0	36.0	36.0	36.0
9	35.17346982186985	36.0	36.0	36.0	36.0	36.0
10	35.023375150017465	36.0	36.0	36.0	36.0	36.0
11	35.176198367074676	36.0	36.0	36.0	36.0	36.0
12	35.08436115539052	36.0	36.0	36.0	36.0	36.0
13	35.124846568572096	36.0	36.0	36.0	36.0	36.0
14	35.06564107681682	36.0	36.0	36.0	36.0	36.0
15	35.03747272111327	36.0	36.0	36.0	36.0	36.0
16	35.05567492758457	36.0	36.0	36.0	36.0	36.0
17	35.02264876348844	36.0	36.0	36.0	36.0	36.0
18	35.00577327601375	36.0	36.0	36.0	36.0	36.0
19	35.012551307942196	36.0	36.0	36.0	36.0	36.0
20	35.003936038068325	36.0	36.0	36.0	36.0	36.0
21	35.0060542715257	36.0	36.0	36.0	36.0	36.0
22	34.996380147535774	36.0	36.0	36.0	36.0	36.0
23	34.947912886139044	36.0	36.0	36.0	36.0	36.0
24	34.91506424817944	36.0	36.0	36.0	32.0	36.0
25	34.88934396739402	36.0	36.0	36.0	32.0	36.0
26	34.82994781887082	36.0	36.0	36.0	32.0	36.0
27	34.827698804324704	36.0	36.0	36.0	32.0	36.0
28	34.80814571849502	36.0	36.0	36.0	32.0	36.0
29	34.792066997733656	36.0	36.0	36.0	32.0	36.0
30	34.77408170929307	36.0	36.0	36.0	32.0	36.0
31	34.74287072368162	36.0	36.0	36.0	32.0	36.0
32	34.72313223333657	36.0	36.0	36.0	32.0	36.0
33	34.7113120389507	36.0	36.0	36.0	32.0	36.0
34	34.702371654643386	36.0	36.0	36.0	32.0	36.0
35	34.65924750977575	36.0	36.0	36.0	32.0	36.0
36	34.630074923382764	36.0	36.0	36.0	32.0	36.0
37	34.62799082956703	36.0	36.0	36.0	32.0	36.0
38	34.60043173516042	36.0	36.0	36.0	32.0	36.0
39	34.60444130476458	36.0	36.0	36.0	32.0	36.0
40	34.57835546720099	36.0	36.0	36.0	32.0	36.0
41	34.55590944066137	36.0	36.0	36.0	32.0	36.0
42	34.50101027077988	36.0	36.0	36.0	32.0	36.0
43	34.50614487288236	36.0	36.0	36.0	32.0	36.0
44	34.44515781705879	36.0	36.0	36.0	32.0	36.0
45	34.40297172449834	36.0	36.0	36.0	32.0	36.0
46	34.375181005753845	36.0	36.0	36.0	32.0	36.0
47	34.37210581188007	36.0	36.0	36.0	32.0	36.0
48	34.31182255789952	36.0	36.0	36.0	32.0	36.0
49	34.322651127002096	36.0	36.0	36.0	32.0	36.0
50	33.69655005790609	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	1.0
20	17.0
21	39.0
22	137.0
23	457.0
24	1139.0
25	2745.0
26	5857.0
27	11118.0
28	19326.0
29	30570.0
30	45368.0
31	66693.0
32	99582.0
33	164079.0
34	348758.0
35	1108056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.522511231584694	17.974895248040347	12.025477693623696	25.477115826751263
2	16.6009169421047	19.770874771591707	36.30335457528269	27.324853711020904
3	18.71226899195247	22.9637729800025	27.973547457952936	30.350410570092095
4	12.172305397477343	15.17843215008837	34.99549619342996	37.65376625900433
5	14.302671558264551	36.53246286000908	32.81392057018454	16.350945011541825
6	35.261547382320224	35.818288200561156	15.477132133504348	13.44303228361428
7	32.01375670713004	30.129720201854678	19.055234817830776	18.801288273184504
8	29.480263347943346	31.733217083476678	18.58677640373015	20.199743164849828
9	27.370296409824373	13.168424270372988	18.203830206973198	41.25744911282944
10	15.712734784474405	27.51465786240754	29.86637617933881	26.90623117377925
11	38.5625109969038	21.4948436010494	20.434571376799223	19.50807402524758
12	25.132461879130897	23.30430937044367	28.257711361258526	23.30551738916691
13	29.45082626331914	19.28192851324075	24.92288331574693	26.34436190769318
14	24.049615034935467	19.499574303588506	24.26879222496099	32.18201843651504
15	25.997920015127164	27.057819377475234	21.540291828180642	25.403968779216957
16	27.067848857332994	25.928044358936788	22.528274515303405	24.47583226842682
17	24.69903279769111	25.22026634172731	24.136884206213942	25.94381665436764
18	24.647861432462612	26.209676995784527	24.550431789270448	24.592029782482413
19	25.93884063817003	25.187978217846744	24.296723012861722	24.576458131121505
20	26.410607101514127	24.49798627261898	24.32319011393264	24.76821651193425
21	27.390864757122714	23.3591306471563	24.525971075845156	24.72403351987584
22	27.026439341934083	23.33467966215375	24.837770878645003	24.801110117267168
23	25.312420722896885	23.469909234774725	25.259477405336245	25.958192636992145
24	25.822952217981886	25.447152165669262	23.347503962839124	25.38239165350973
25	25.651332393160374	24.41555644845725	24.808958430173774	25.124152728208603
26	25.231727449545737	24.824973356661463	25.44377113148967	24.49952806230313
27	25.683822166054476	24.264781195290944	24.550207188595987	25.501189450058593
28	25.259445235231397	24.21667445781487	24.97344881740875	25.550431489544977
29	25.31502491994052	24.93259876978639	25.129248063314353	24.623128246958736
30	25.169891515246377	25.081754875090738	24.804528899678655	24.943824709984234
31	25.576200133521798	24.602835683621624	23.809898997220834	26.011065185635747
32	25.203583831788073	23.551119711074833	23.875929415867773	27.36936704126932
33	25.034298596331254	23.535665287719524	25.37817747869075	26.051858637258462
34	25.34755049645569	24.083983578934983	25.53705664595125	25.031409278658078
35	26.18299280424392	23.79242607279794	25.02274279111298	25.00183833184516
36	25.760007900006094	23.9155350678329	24.570174535506954	25.754282496654053
37	25.886192167977327	23.70117591691896	24.40783958619154	26.00479232891218
38	25.418661355201106	24.253153945307453	24.243226463200557	26.084958236290884
39	26.694791954458886	23.76353958427659	24.43632994408208	25.10533851718244
40	26.16491088559752	24.588515894471406	24.031449114213395	25.215124105717685
41	25.211356526525663	25.22732381878804	24.67603204383442	24.885287610851876
42	25.825692072872886	24.21180954221768	24.258187590011755	25.70431079489768
43	24.98057957175789	24.290425610699593	24.8567300253214	25.87226479222111
44	25.60740536094517	24.5738524453259	24.19437181643327	25.624370377295662
45	26.373899378336528	24.60549895899206	23.600154207836027	25.420447454835383
46	25.93367207056547	24.2242640763808	23.56084822267999	26.281215630373744
47	25.249841772567432	23.80423392973914	24.83427481938437	26.111649478309058
48	25.560648580737226	24.52546363774357	23.954060877845656	25.959826903673545
49	25.916442657271404	24.326264825850423	24.321327690292435	25.43596482658574
50	25.646416680541556	25.53611787056067	24.026652394350812	24.790813054546966
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	28.5
2	44.0
3	136.0
4	228.0
5	267.5
6	307.0
7	280.5
8	254.0
9	336.5
10	419.0
11	643.0
12	867.0
13	1541.5
14	2216.0
15	2947.0
16	3678.0
17	3588.5
18	3499.0
19	3354.5
20	3210.0
21	3508.0
22	3806.0
23	4236.5
24	4667.0
25	5927.5
26	7188.0
27	8858.5
28	10529.0
29	12236.5
30	13944.0
31	15920.0
32	17896.0
33	21021.5
34	24147.0
35	28701.0
36	33255.0
37	44588.0
38	55921.0
39	65100.5
40	74280.0
41	88011.5
42	101743.0
43	107671.5
44	113600.0
45	124758.0
46	135916.0
47	138306.5
48	140697.0
49	152917.5
50	165138.0
51	154384.5
52	143631.0
53	144536.5
54	145442.0
55	141597.5
56	137753.0
57	139223.5
58	140694.0
59	133009.5
60	125325.0
61	108980.5
62	92636.0
63	78131.0
64	63626.0
65	54809.0
66	45992.0
67	38615.0
68	31238.0
69	28744.5
70	26251.0
71	21550.0
72	16849.0
73	12428.5
74	8008.0
75	5650.0
76	3292.0
77	2779.5
78	2267.0
79	1846.5
80	1426.0
81	1148.0
82	870.0
83	742.0
84	614.0
85	441.0
86	268.0
87	192.0
88	116.0
89	85.5
90	55.0
91	47.0
92	39.0
93	30.5
94	22.0
95	28.5
96	35.0
97	26.0
98	17.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008508649146902879
2	4.201802047853273E-4
3	3.676576791871614E-4
4	0.0
5	0.0
6	3.676576791871614E-4
7	5.252252559816591E-5
8	0.0
9	0.0014181081911504795
10	9.454054607669865E-4
11	0.0
12	5.252252559816591E-5
13	6.30270307177991E-4
14	3.151351535889955E-4
15	0.004464414675844103
16	0.00115549556315965
17	0.0
18	3.676576791871614E-4
19	5.252252559816591E-5
20	6.827928327761568E-4
21	0.0
22	1.0504505119633182E-4
23	0.00115549556315965
24	3.676576791871614E-4
25	0.002100901023926637
26	0.004832072355031264
27	0.006512793174172572
28	0.0039391894198624435
29	0.002521081228711964
30	0.004359369624647771
31	0.007143063481350565
32	0.0032038740614881204
33	0.004044234471058775
34	0.00257360375431013
35	0.002363513651917466
36	0.007825856314126722
37	0.0036240542662734484
38	0.007300631058145063
39	0.004359369624647771
40	0.0023109911263193
41	0.002783693856702793
42	0.0014181081911504795
43	0.00115549556315965
44	0.0014181081911504795
45	0.0011029730375614842
46	5.77747781579825E-4
47	0.002100901023926637
48	0.0017857658703376413
49	3.676576791871614E-4
50	0.0013655856655523137
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1903945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.58237735629537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.98317447539713	28.400835951520246
2	15.60729971139918	12.667626528016026
3	5.984137359355705	7.285515614078343
4	2.8351549294386933	4.602293088401681
5	1.5366444231667946	3.1180341921700845
6	0.9274383319708346	2.2582591417640123
7	0.6182889252530929	1.756414413588752
8	0.41657449577508343	1.3524466707642324
9	0.3096468208563614	1.1309583718053045
>10	1.4829622252573105	10.72487370747684
>50	0.13442830209228335	3.85953314959992
>100	0.13535711786497462	11.50013441155556
>500	0.019652365802127904	5.588271063315289
>1k	0.009240516370536963	5.754803695943857
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCTGGTCAGTGCCAGTTCTGAGTTAATCTTTCATTGGAGCCCGAGACA	3473	0.18241073140243022	No Hit
GTCATACACCGGCGCAGAAGCGTTGTTCGAAGGTCTTGGCTGAAGGCACC	2891	0.15184262150429764	No Hit
GTTCTAAGTTGGTTGTTAAGCGTACACCAGACGTCCGAAGACAGCCAAGG	2886	0.15158000887630682	No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA	2800	0.14706307167486457	No Hit
GTCCTTGACAGTCCTTGAAAATCCAAGGGAATGAATAATTTTTGCGCCTG	2700	0.14181081911504798	No Hit
GTCCCAATCAGTCCGGCCGTTTCCAACCAAGGCCCGATGCACCCAACCCT	2169	0.11392135802242187	No Hit
GTCTACCACCCTGAAATCGGATTATCCGGAGCTAGGGTTCAATGGCTGGT	2149	0.11287090751045854	No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA	2089	0.1097195559745686	No Hit
GACTAATGTAGGTAAGGGAAGTCGGCAAATTGGATCCGTAACTTCGGAAA	2064	0.10840649283461444	No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAAAACCACTT	2063	0.10835397030901628	No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAG	1998	0.10494000614513549	No Hit
GTCAAGGACGCACCGGACTCGACGGAGGTGCCGAGCTCTACCAGCCATTG	1971	0.10352189795398502	No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCATCACCCGTG	1951	0.1024714474420217	No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG	1948	0.10231387986522719	No Hit
GTGTATGACCGGGATCCACCTTGCGGCATCACCCAGCCCGCCCGGCTCCA	1944	0.10210378976283453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	5.252252559816591E-5	0.0
24	0.0	0.0	0.0	5.252252559816591E-5	0.0
25	0.0	0.0	0.0	5.252252559816591E-5	0.0
26	0.0	0.0	0.0	5.252252559816591E-5	0.0
27	0.0	0.0	0.0	5.252252559816591E-5	0.0
28	0.0	0.0	0.0	1.5756757679449775E-4	0.0
29	0.0	0.0	0.0	4.7270273038349324E-4	0.0
30	0.0	0.0	0.0	0.001890810921533973	0.0
31	0.0	0.0	0.0	0.005304775085414757	0.0
32	0.0	0.0	0.0	0.008823784300491874	0.0
33	0.0	0.0	0.0	0.012185225938774491	0.0
34	0.0	0.0	0.0	0.017279910921796586	0.0
35	0.0	0.0	0.0	0.0232674788399875	0.0
36	0.0	0.0	0.0	0.03277405597325553	0.0
37	0.0	0.0	0.0	0.04916108395988329	0.0
38	0.0	0.0	0.0	0.07237604027427263	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1475	0.0	29.831932	1
GTATCAA	3950	0.0	28.40642	1
ATCAACG	6135	0.0	18.14403	3
ATACCGT	355	0.0	17.970802	6
AACGCAG	6195	0.0	17.93279	6
TCAACGC	6235	0.0	17.853027	4
CAACGCA	6325	0.0	17.598993	5
GTCATAC	1035	0.0	17.218214	1
CATACAC	1155	0.0	17.141874	3
TAATACG	105	3.7780366E-5	16.760944	4
AACCGTA	160	2.132765E-8	16.499054	7
TATCAAC	6915	0.0	16.383732	2
TACACCG	1060	0.0	16.187752	5
TAGCGGC	410	0.0	16.099174	30
ATACACC	1090	0.0	15.944041	4
GTATAAT	140	1.586508E-6	15.715035	1
ACGGACC	700	0.0	15.713386	8
TACCGTC	380	0.0	15.630683	7
AAGACGG	720	0.0	15.58244	5
GTATTAG	325	0.0	15.569973	1
>>END_MODULE