FastQCFastQC Report
Thu 2 Feb 2017
SRR4064208_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064208_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170693
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39510.1820156051546672No Hit
TATCAACGCAGAGTACTTTTTTTTT24470.11272897641444461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC307.73871E-418.9946543
CGACCAT2250.013.51166810
CGAACGA1102.743036E-912.95627416
GTAAACG1352.7284841E-1112.66864917
GGTATCA9000.012.454061
GTATCAA24400.012.4185911
CGTCTTA853.9384486E-612.29604215
GTGTACG701.0925089E-412.2128191
CGGTCCA3200.012.17237710
ACGAACG1209.997166E-911.87685915
CCGACCA2800.011.87521659
TACCCCG658.036867E-411.6903655
GTATTAG2700.011.2579061
ACGCCCC1701.0913936E-1111.1782217
CAAGACG4100.011.1203594
TGTTATA1201.2826058E-711.0791932
CGTCGTA2750.011.05500110
AACGAAC1303.2627213E-810.962749514
AAGACGG3900.010.9597175
ATTAGGC700.001495321510.8540883
Produced by FastQC (version 0.11.5)