##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064208_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2170693
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21014348873839	32.0	32.0	32.0	32.0	32.0
2	30.79039458827204	32.0	32.0	32.0	32.0	32.0
3	30.804835598585335	32.0	32.0	32.0	32.0	32.0
4	30.837070004832558	32.0	32.0	32.0	32.0	32.0
5	30.735767333289417	32.0	32.0	32.0	32.0	32.0
6	34.37367191030698	36.0	36.0	36.0	32.0	36.0
7	34.31792335443105	36.0	36.0	36.0	32.0	36.0
8	34.27364118279278	36.0	36.0	36.0	32.0	36.0
9	34.43080435602823	36.0	36.0	36.0	32.0	36.0
10	34.107068111428006	36.0	36.0	36.0	32.0	36.0
11	34.403437519722964	36.0	36.0	36.0	32.0	36.0
12	34.21023055770669	36.0	36.0	36.0	32.0	36.0
13	34.29609253818942	36.0	36.0	36.0	32.0	36.0
14	34.196384288335565	36.0	36.0	36.0	32.0	36.0
15	34.12220290939345	36.0	36.0	36.0	32.0	36.0
16	34.12632463457523	36.0	36.0	36.0	32.0	36.0
17	34.0512780941386	36.0	36.0	36.0	32.0	36.0
18	34.04431119462771	36.0	36.0	36.0	32.0	36.0
19	34.06510916099144	36.0	36.0	36.0	32.0	36.0
20	34.046680484066606	36.0	36.0	36.0	32.0	36.0
21	34.04049075571719	36.0	36.0	36.0	32.0	36.0
22	34.013556039476796	36.0	36.0	36.0	32.0	36.0
23	33.95044808270907	36.0	36.0	36.0	32.0	36.0
24	33.93465404826938	36.0	36.0	36.0	32.0	36.0
25	33.52142656746025	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	24.0
5	24.0
6	79.0
7	38.0
8	112.0
9	146.0
10	193.0
11	63.0
12	123.0
13	103.0
14	170.0
15	311.0
16	518.0
17	753.0
18	983.0
19	1274.0
20	2051.0
21	3085.0
22	4859.0
23	7935.0
24	11672.0
25	17447.0
26	25226.0
27	33792.0
28	45884.0
29	62939.0
30	82982.0
31	112208.0
32	158092.0
33	220783.0
34	455952.0
35	920864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63311673349002	16.900778837734457	11.16774966588322	26.298354762892302
2	16.8795042456179	19.255493089209345	37.20968160284457	26.655321062328184
3	18.96962613485644	22.977367598354228	28.523358682126865	29.529647584662467
4	12.624152945095132	15.036240981217713	35.79506949432164	36.54453657936551
5	14.622120371931707	36.324248815713936	33.88748982956048	15.166140982793873
6	34.8270701772654	35.393044104868274	16.45494093764427	13.324944780222058
7	30.540041276864176	30.31960310069518	20.531530630723204	18.60882499171744
8	28.205749741010962	33.19760218546196	18.97868916522579	19.61795890830129
9	27.132303868971903	14.010366543883132	18.289584785165683	40.56774480197928
10	15.400259049445255	26.86817517642558	32.04810391478103	25.683461859348135
11	37.498882244324335	20.97421280039972	22.21051971721012	19.316385238065827
12	24.3645413570157	23.544167959664016	28.944656025828603	23.146634657491685
13	29.427074210042058	19.462227499774126	25.119620753808963	25.991077536374853
14	23.34073662268025	19.317468351629508	25.26863430055511	32.07316072513514
15	25.183468752117477	27.121800388394625	22.164260595922546	25.53047026356535
16	25.788844098103347	25.82664352604127	23.466530219951185	24.917982155904202
17	24.141081458570053	25.904853218769375	25.082638627998254	24.87142669466231
18	24.777523243478985	25.23116698469367	25.747048434306713	24.244261337520626
19	25.414032001386715	25.117280638183697	25.27254840131369	24.196138959115896
20	25.670135269517864	24.472298585988415	24.876341411577364	24.981224732916353
21	26.485598111711443	24.316371314241458	24.597724464769772	24.600306109277323
22	25.74220269938831	24.25383249334742	25.013369698914378	24.990595108349883
23	24.4905580521081	24.178246936017715	25.470322375703724	25.860872636170456
24	24.674531361581263	24.946394892266525	25.178426289576844	25.20064745657537
25	24.808547775346128	24.5451179970854	25.255235055984592	25.391099171583885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	96.0
2	267.0
3	438.0
4	438.0
5	438.0
6	1378.5
7	2319.0
8	2319.0
9	2319.0
10	2904.5
11	3490.0
12	3490.0
13	3490.0
14	4793.5
15	6097.0
16	6097.0
17	6097.0
18	10149.0
19	14201.0
20	14201.0
21	14201.0
22	22043.0
23	29885.0
24	29885.0
25	29885.0
26	43765.0
27	57645.0
28	57645.0
29	57645.0
30	72908.0
31	88171.0
32	88171.0
33	88171.0
34	109530.0
35	130889.0
36	130889.0
37	130889.0
38	153518.5
39	176148.0
40	176148.0
41	176148.0
42	202763.0
43	229378.0
44	229378.0
45	229378.0
46	255533.5
47	281689.0
48	281689.0
49	281689.0
50	289340.5
51	296992.0
52	296992.0
53	296992.0
54	280849.0
55	264706.0
56	264706.0
57	264706.0
58	241404.5
59	218103.0
60	218103.0
61	218103.0
62	190375.5
63	162648.0
64	162648.0
65	162648.0
66	132664.5
67	102681.0
68	102681.0
69	102681.0
70	77275.0
71	51869.0
72	51869.0
73	51869.0
74	39247.0
75	26625.0
76	26625.0
77	26625.0
78	21005.0
79	15385.0
80	15385.0
81	15385.0
82	10527.0
83	5669.0
84	5669.0
85	5669.0
86	4224.0
87	2779.0
88	2779.0
89	2779.0
90	1966.5
91	1154.0
92	1154.0
93	1154.0
94	812.0
95	470.0
96	470.0
97	470.0
98	818.0
99	1166.0
100	1166.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03653211209507747
2	0.004698960193818288
3	0.002027002436549065
4	0.003869731924320943
5	0.008614760355333527
6	0.012668765228431658
7	0.021652071481319562
8	0.0330309260683109
9	0.04703567017537717
10	0.05634145408862515
11	0.05376163280574452
12	0.06057973191050047
13	0.06159323312877501
14	0.06389664498848985
15	0.05758529649287117
16	0.06209998373791228
17	0.06025725425014039
18	0.07324849713893213
19	0.0708068805676344
20	0.07444627130598384
21	0.0706226076188572
22	0.07412379364562377
23	0.07840813970469339
24	0.07324849713893213
25	0.07435413483159525
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2170693.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.79038328393687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73130703930732	41.811967988057205
2	12.939543844835915	13.920460458660603
3	3.897104236529941	6.288801917411992
4	1.70300721954319	3.664216442981593
5	0.9231757181385558	2.4828987858548297
6	0.5762007248639713	1.8596434703349163
7	0.3919345753820423	1.4757617722418972
8	0.27879939281594496	1.1997380959118846
9	0.22824246769410686	1.104952483704383
>10	1.1813362776661438	11.558877178129663
>50	0.07949814049671512	2.92962827454294
>100	0.05951846415709046	6.661320213183537
>500	0.007404527307733872	2.72515339070468
>1k	0.0029273712611970915	2.316579528279925
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3951	0.1820156051546672	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2447	0.11272897641444461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.606823719429694E-5
2	0.0	0.0	0.0	0.0	4.606823719429694E-5
3	0.0	0.0	0.0	0.0	4.606823719429694E-5
4	0.0	0.0	0.0	0.0	4.606823719429694E-5
5	0.0	4.606823719429694E-5	0.0	0.0	9.213647438859388E-5
6	0.0	4.606823719429694E-5	0.0	0.0	1.3820471158289082E-4
7	0.0	9.213647438859388E-5	0.0	0.0	1.3820471158289082E-4
8	0.0	9.213647438859388E-5	0.0	0.0	1.3820471158289082E-4
9	0.0	9.213647438859388E-5	0.0	4.606823719429694E-5	1.3820471158289082E-4
10	0.0	9.213647438859388E-5	0.0	4.606823719429694E-5	1.3820471158289082E-4
11	0.0	9.213647438859388E-5	0.0	4.606823719429694E-5	1.3820471158289082E-4
12	0.0	9.213647438859388E-5	0.0	4.606823719429694E-5	2.303411859714847E-4
13	0.0	9.213647438859388E-5	0.0	4.606823719429694E-5	3.2247766036007857E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAC	30	7.73871E-4	18.994654	3
CGACCAT	225	0.0	13.511668	10
CGAACGA	110	2.743036E-9	12.956274	16
GTAAACG	135	2.7284841E-11	12.668649	17
GGTATCA	900	0.0	12.45406	1
GTATCAA	2440	0.0	12.418591	1
CGTCTTA	85	3.9384486E-6	12.296042	15
GTGTACG	70	1.0925089E-4	12.212819	1
CGGTCCA	320	0.0	12.172377	10
ACGAACG	120	9.997166E-9	11.876859	15
CCGACCA	280	0.0	11.8752165	9
TACCCCG	65	8.036867E-4	11.690365	5
GTATTAG	270	0.0	11.257906	1
ACGCCCC	170	1.0913936E-11	11.17822	17
CAAGACG	410	0.0	11.120359	4
TGTTATA	120	1.2826058E-7	11.079193	2
CGTCGTA	275	0.0	11.055001	10
AACGAAC	130	3.2627213E-8	10.9627495	14
AAGACGG	390	0.0	10.959717	5
ATTAGGC	70	0.0014953215	10.854088	3
>>END_MODULE