FastQCFastQC Report
Thu 2 Feb 2017
SRR4064208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170693
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC551.594754E-423.9987456
GTATCAA27350.022.9259571
GGTATCA13950.020.9757441
GTATTAC2200.018.0007151
CTATTAC1601.1023076E-917.8757111
ATACGAA5350.017.27012440
CAATACG5750.017.21728338
TACACCG1551.441731E-817.0313665
CGAATGC5600.016.89158443
CGCAATA6000.016.86733836
CGTCGTA5250.016.76218610
TCTATCG800.001988508516.50065636
AACGCAG37500.016.484476
ACCGTCG5100.016.3916768
TCTAGCG5950.016.2706828
GTACTAG1501.8387254E-716.1339761
CCGTCGT5200.016.0768249
GTATAGG2750.016.0006371
CGCCGGT6350.015.936547
TAGCGGC6100.015.87021430