##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064208_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2170693
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308639222589285	32.0	32.0	32.0	32.0	32.0
2	31.34714397660102	32.0	32.0	32.0	32.0	32.0
3	31.437195863256573	32.0	32.0	32.0	32.0	32.0
4	31.527807939676407	32.0	32.0	32.0	32.0	32.0
5	31.47405736324759	32.0	32.0	32.0	32.0	32.0
6	35.05357782053934	36.0	36.0	36.0	36.0	36.0
7	35.067132017286646	36.0	36.0	36.0	36.0	36.0
8	35.004106061981126	36.0	36.0	36.0	36.0	36.0
9	35.129337036605364	36.0	36.0	36.0	36.0	36.0
10	34.96753387051969	36.0	36.0	36.0	36.0	36.0
11	35.12572160135035	36.0	36.0	36.0	36.0	36.0
12	35.027864833949344	36.0	36.0	36.0	36.0	36.0
13	35.07214792695236	36.0	36.0	36.0	36.0	36.0
14	35.018178526396866	36.0	36.0	36.0	36.0	36.0
15	34.98979726750858	36.0	36.0	36.0	36.0	36.0
16	35.0028898605192	36.0	36.0	36.0	36.0	36.0
17	34.964449141357164	36.0	36.0	36.0	36.0	36.0
18	34.96125476979011	36.0	36.0	36.0	36.0	36.0
19	34.96042876629721	36.0	36.0	36.0	36.0	36.0
20	34.95873253380372	36.0	36.0	36.0	36.0	36.0
21	34.955521116988905	36.0	36.0	36.0	36.0	36.0
22	34.938426115530845	36.0	36.0	36.0	36.0	36.0
23	34.88321287257111	36.0	36.0	36.0	32.0	36.0
24	34.85934860433972	36.0	36.0	36.0	32.0	36.0
25	34.82867360792152	36.0	36.0	36.0	32.0	36.0
26	34.771103513946926	36.0	36.0	36.0	32.0	36.0
27	34.76486080712473	36.0	36.0	36.0	32.0	36.0
28	34.744960250021535	36.0	36.0	36.0	32.0	36.0
29	34.72276595538844	36.0	36.0	36.0	32.0	36.0
30	34.70062279649863	36.0	36.0	36.0	32.0	36.0
31	34.698208820869645	36.0	36.0	36.0	32.0	36.0
32	34.66137035499723	36.0	36.0	36.0	32.0	36.0
33	34.64422652120774	36.0	36.0	36.0	32.0	36.0
34	34.6369666277083	36.0	36.0	36.0	32.0	36.0
35	34.599568893436334	36.0	36.0	36.0	32.0	36.0
36	34.57936705006189	36.0	36.0	36.0	32.0	36.0
37	34.57288570977103	36.0	36.0	36.0	32.0	36.0
38	34.53381063098283	36.0	36.0	36.0	32.0	36.0
39	34.54271331782062	36.0	36.0	36.0	32.0	36.0
40	34.518445031149035	36.0	36.0	36.0	32.0	36.0
41	34.508487842361866	36.0	36.0	36.0	32.0	36.0
42	34.453558840425615	36.0	36.0	36.0	32.0	36.0
43	34.44060491280895	36.0	36.0	36.0	32.0	36.0
44	34.38700175473915	36.0	36.0	36.0	32.0	36.0
45	34.356672730782286	36.0	36.0	36.0	32.0	36.0
46	34.34702834532566	36.0	36.0	36.0	32.0	36.0
47	34.32969056425759	36.0	36.0	36.0	32.0	36.0
48	34.2855005290937	36.0	36.0	36.0	32.0	36.0
49	34.272014052655074	36.0	36.0	36.0	32.0	36.0
50	33.65700308611121	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	4.0
20	15.0
21	48.0
22	207.0
23	618.0
24	1598.0
25	3766.0
26	7483.0
27	14403.0
28	23932.0
29	37737.0
30	55427.0
31	80314.0
32	116329.0
33	189501.0
34	394516.0
35	1244791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6711590097802	17.849692330090466	11.736607282719067	26.742541377410266
2	16.10009969244604	20.03011964936269	36.691473071061736	27.17830758712954
3	18.160417342335702	23.636561311436743	28.900606627944686	29.302414718282865
4	12.268201906027246	15.748749362530766	35.83325693683999	36.149791794601995
5	14.303680898220062	36.771021973167095	33.62474564574539	15.300551482867453
6	34.9112515991481	35.72656951462832	16.22057901484645	13.141599871377126
7	30.487729033999738	30.436915768374433	20.515337728550286	18.560017469075543
8	28.16364551027967	33.46748410184402	18.839383938393837	19.52948644948247
9	27.203917317656444	13.861671217491015	18.10580056194657	40.82861090290597
10	15.248078125295129	27.085914145283454	32.14048953616561	25.52551819325581
11	37.743107846203955	20.887707289791784	22.084606160336815	19.284578703667446
12	24.35527353539523	23.574691526373208	28.903462865229823	23.16657207300174
13	29.556186385317822	19.37723834769285	25.127678531118736	25.9388967358706
14	23.36753595974738	19.175460717347608	25.240073322273876	32.21693000063113
15	25.158965409532303	27.223649888187495	22.03203909705989	25.58534560522031
16	25.796836655108063	25.903992164656465	23.392103016708614	24.907068163526862
17	24.187344778833303	25.999254615922197	25.004272828999767	24.80912777624473
18	24.92923902467789	25.19569832237521	25.685358743403015	24.189703909543887
19	25.45666291824777	25.087472065372673	25.200108905312728	24.255756111066834
20	25.822367511807336	24.464316316884446	24.77417256496109	24.939143606347123
21	26.58123465639775	24.423214153268102	24.454172008662674	24.541379181671473
22	25.798593259660972	24.344494750982637	24.925830103948417	24.93108188540797
23	24.58331029907679	24.13464905006726	25.37347743564229	25.908563215213665
24	24.842929549330577	24.948334210169893	25.012323315897955	25.196412924601574
25	24.954909840411933	24.488920604924513	25.283935556660193	25.27223399800336
26	24.85207891151824	25.142369584577047	25.359638401295875	24.645913102608837
27	25.32931472315081	24.783098248937605	24.752645390515294	25.134941637396295
28	24.83756817805928	24.65969133922693	25.444818789986005	25.05792169272778
29	24.744498847342438	24.917397481659755	25.264162208679853	25.073941462317954
30	24.678329974085806	25.03979268643824	25.393976389288795	24.88790095018716
31	25.13419416009498	24.827128989025358	24.754704915668803	25.283971935210865
32	25.01140221963209	24.841589768868946	24.644273782265977	25.50273422923299
33	24.68207967323185	24.535852217207204	25.098786459540285	25.68328165002066
34	25.21679080050547	24.660181024867768	25.343527557067247	24.779500617559513
35	25.63742632943326	24.46722008179143	25.288221047864617	24.60713254091069
36	24.804980484227055	25.029854156914922	24.886065848687338	25.27909951017068
37	25.51837628258049	24.844376547941973	24.85898064335417	24.778266526123364
38	24.758791631536997	24.63863664634905	25.26318456782445	25.339387154289504
39	25.442516098090895	24.691847734196873	24.65618937445378	25.20944679325845
40	25.499437953783215	24.807706482880626	25.037316183245494	24.655539380090666
41	24.61882209855158	25.088407105738398	25.469400729738695	24.823370065971325
42	25.684171052437605	25.016446641015435	24.94066309908157	24.35871920746539
43	25.025337962348708	24.266166541358924	25.289083115887546	25.419412380404815
44	24.742175428509828	24.806994363931373	24.989887877523078	25.460942330035724
45	24.823936281219147	25.03060124948	25.014477143619253	25.1309853256816
46	24.764013389389657	24.750146728619775	25.09146928557674	25.394370596413836
47	24.76542578554202	24.79205393813331	25.466372743459864	24.976147532864807
48	25.475688759362434	25.102757617331644	24.647085977328366	24.77446764597755
49	24.753015297964094	25.213101326265814	24.89928275965008	25.13460061612001
50	24.887856740412253	25.20462660912966	24.8759709599979	25.031545690460188
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	28.0
2	40.0
3	129.0
4	218.0
5	229.0
6	240.0
7	228.0
8	216.0
9	307.5
10	399.0
11	595.5
12	792.0
13	1509.5
14	2227.0
15	2938.0
16	3649.0
17	3825.0
18	4001.0
19	4236.5
20	4472.0
21	5397.5
22	6323.0
23	7698.5
24	9074.0
25	11401.5
26	13729.0
27	17022.0
28	20315.0
29	23656.5
30	26998.0
31	30257.0
32	33516.0
33	38432.0
34	43348.0
35	49950.0
36	56552.0
37	66130.5
38	75709.0
39	83806.5
40	91904.0
41	100732.0
42	109560.0
43	115805.5
44	122051.0
45	133044.0
46	144037.0
47	153002.0
48	161967.0
49	167726.0
50	173485.0
51	170108.5
52	166732.0
53	164786.0
54	162840.0
55	161057.0
56	159274.0
57	153360.0
58	147446.0
59	135653.0
60	123860.0
61	108197.0
62	92534.0
63	79995.0
64	67456.0
65	57575.0
66	47694.0
67	40608.0
68	33522.0
69	29673.0
70	25824.0
71	19991.5
72	14159.0
73	11956.5
74	9754.0
75	7550.5
76	5347.0
77	4587.0
78	3827.0
79	3185.0
80	2543.0
81	2017.0
82	1491.0
83	1142.0
84	793.0
85	580.0
86	367.0
87	255.5
88	144.0
89	116.0
90	88.0
91	72.0
92	56.0
93	43.0
94	30.0
95	36.0
96	42.0
97	30.0
98	18.0
99	16.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008522623880944933
2	7.83160032303048E-4
3	2.303411859714847E-4
4	0.0
5	0.0
6	2.7640942316578163E-4
7	0.0
8	4.606823719429694E-5
9	0.0011056376926631265
10	0.0011977741670517203
11	0.0
12	2.303411859714847E-4
13	3.2247766036007857E-4
14	9.213647438859388E-5
15	0.004376482533458208
16	7.37091795108751E-4
17	0.0
18	2.303411859714847E-4
19	0.0
20	4.146141347486724E-4
21	0.0
22	4.606823719429694E-5
23	5.988870835258602E-4
24	5.067506091372663E-4
25	0.0019348659621604715
26	0.005067506091372663
27	0.005758529649287117
28	0.004146141347486724
29	0.0026258895200749256
30	0.003132640129212192
31	0.006633826155978758
32	0.002902298943240707
33	0.004698960193818288
34	0.0023955483341034406
35	0.002303411859714847
36	0.006495621444395867
37	0.0031787083664064886
38	0.0075091226626704
39	0.004330414296263912
40	0.0024416165712977376
41	0.0024416165712977376
42	0.0017045247761889866
43	0.0017045247761889866
44	0.001428115353023205
45	0.001382047115828908
46	8.752965066916418E-4
47	0.0026719577572692226
48	0.002349480096909144
49	5.528188463315633E-4
50	0.001474183590217502
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2170693.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.70600831935397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6839582788197	44.61853192758451
2	12.619170047487854	14.311655233923776
3	3.776020539913632	6.4236915645118176
4	1.5713843496661397	3.5642773602028304
5	0.8214869287660328	2.32916223084236
6	0.5208449121334628	1.7721021552320002
7	0.34896080293647697	1.3851721936111017
8	0.24692798930677065	1.1201840492728872
9	0.19927612461836547	1.0170138222411886
>10	1.0860133484661427	10.988193652474196
>50	0.07038836107342741	2.7651080388881826
>100	0.047387479234881576	5.709267910763099
>500	0.006053819806971148	2.368408557145803
>1k	0.0021270177700168525	1.6272313033063468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	4.606823719429694E-5	0.0	0.0	0.0
4	0.0	4.606823719429694E-5	0.0	0.0	0.0
5	0.0	4.606823719429694E-5	0.0	0.0	0.0
6	0.0	4.606823719429694E-5	0.0	0.0	0.0
7	0.0	4.606823719429694E-5	0.0	0.0	0.0
8	0.0	4.606823719429694E-5	0.0	0.0	0.0
9	0.0	4.606823719429694E-5	0.0	0.0	0.0
10	0.0	4.606823719429694E-5	0.0	0.0	0.0
11	0.0	4.606823719429694E-5	0.0	0.0	0.0
12	0.0	4.606823719429694E-5	0.0	0.0	9.213647438859388E-5
13	0.0	4.606823719429694E-5	0.0	0.0	1.3820471158289082E-4
14	0.0	1.3820471158289082E-4	0.0	0.0	1.3820471158289082E-4
15	0.0	1.8427294877718776E-4	0.0	4.606823719429694E-5	1.8427294877718776E-4
16	0.0	1.8427294877718776E-4	0.0	4.606823719429694E-5	1.8427294877718776E-4
17	0.0	1.8427294877718776E-4	0.0	1.8427294877718776E-4	1.8427294877718776E-4
18	0.0	2.303411859714847E-4	0.0	2.303411859714847E-4	1.8427294877718776E-4
19	0.0	2.303411859714847E-4	0.0	2.303411859714847E-4	1.8427294877718776E-4
20	0.0	2.303411859714847E-4	0.0	2.7640942316578163E-4	2.7640942316578163E-4
21	0.0	2.303411859714847E-4	0.0	3.2247766036007857E-4	2.7640942316578163E-4
22	0.0	2.303411859714847E-4	0.0	4.1461413474867245E-4	2.7640942316578163E-4
23	0.0	2.303411859714847E-4	0.0	5.988870835258602E-4	2.7640942316578163E-4
24	0.0	2.303411859714847E-4	0.0	0.0011056376926631265	2.7640942316578163E-4
25	0.0	2.303411859714847E-4	0.0	0.001382047115828908	2.7640942316578163E-4
26	0.0	2.303411859714847E-4	0.0	0.0017966612505775806	3.2247766036007857E-4
27	0.0	2.303411859714847E-4	0.0	0.0025337530456863316	3.2247766036007857E-4
28	0.0	2.303411859714847E-4	0.0	0.004698960193818287	3.685458975543755E-4
29	0.0	2.303411859714847E-4	0.0	0.010042875708356732	4.606823719429694E-4
30	0.0	2.303411859714847E-4	0.0	0.018980113724050337	4.606823719429694E-4
31	0.0	2.303411859714847E-4	0.0	0.037269203890186224	5.067506091372663E-4
32	0.0	2.303411859714847E-4	0.0	0.057953842390425545	5.067506091372663E-4
33	0.0	2.303411859714847E-4	0.0	0.07896095855102495	5.067506091372663E-4
34	0.0	2.7640942316578163E-4	0.0	0.1036074654499738	5.528188463315633E-4
35	0.0	2.7640942316578163E-4	0.0	0.13124840776655197	5.528188463315633E-4
36	0.0	2.7640942316578163E-4	0.0	0.17376939069688804	5.528188463315633E-4
37	0.0	3.2247766036007857E-4	0.0	0.23725142155062923	5.528188463315633E-4
38	0.0	3.2247766036007857E-4	0.0	0.3291575547532516	6.449553207201571E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGC	55	1.594754E-4	23.998745	6
GTATCAA	2735	0.0	22.925957	1
GGTATCA	1395	0.0	20.975744	1
GTATTAC	220	0.0	18.000715	1
CTATTAC	160	1.1023076E-9	17.875711	1
ATACGAA	535	0.0	17.270124	40
CAATACG	575	0.0	17.217283	38
TACACCG	155	1.441731E-8	17.031366	5
CGAATGC	560	0.0	16.891584	43
CGCAATA	600	0.0	16.867338	36
CGTCGTA	525	0.0	16.762186	10
TCTATCG	80	0.0019885085	16.500656	36
AACGCAG	3750	0.0	16.48447	6
ACCGTCG	510	0.0	16.391676	8
TCTAGCG	595	0.0	16.27068	28
GTACTAG	150	1.8387254E-7	16.133976	1
CCGTCGT	520	0.0	16.076824	9
GTATAGG	275	0.0	16.000637	1
CGCCGGT	635	0.0	15.93654	7
TAGCGGC	610	0.0	15.870214	30
>>END_MODULE