FastQCFastQC Report
Thu 2 Feb 2017
SRR4064207_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064207_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468261
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40580.27638137905999No Hit
TATCAACGCAGAGTACTTTTTTTTT27010.1839591189849761No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18930.1289280311879155No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15440.10515841529537323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGA551.9606612E-413.8176616
AACCGCG1102.743036E-912.9553817
GCGTAAC658.0186065E-411.69306411
GTCTAGA1157.088238E-811.5632051
CGCCAGT2150.011.48989818
GTATCAA23450.011.2603011
CGCATCG1950.011.20623313
GAATTAG855.3315984E-511.1745261
TACACCG600.005878532311.0825385
GTATTAC600.005883007311.0814061
GTCCTAC1303.2727257E-810.9596321
GCATCGC2100.010.85858314
ACGGTAT1855.456968E-1210.7848489
GTATTAA1158.8331217E-710.7372621
GTCCTAA1158.8331217E-710.7372621
CGTCTTA803.765782E-410.68891815
CGCGTAA803.768155E-410.688189510
TAATACA2400.010.6856414
GATTAAA2500.010.6381481
GGTTCTA2350.010.51167513
Produced by FastQC (version 0.11.5)