FastQCFastQC Report
Thu 2 Feb 2017
SRR4064207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468261
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26700.023.154581
ACGTAAC808.987199E-519.250851
GTATTAC1404.081812E-918.8579751
ACGATTA600.00741386718.33289340
AACGCAG33750.017.8603126
TACCGTC2600.017.7688057
ACTGCGC1002.4833877E-517.6001768
ATACCGT2300.017.216986
ACCGTCG2201.8189894E-1217.000178
CCGTCGT2350.016.8518079
CGTCGTA2350.016.85180710
GTATTAG2950.016.4075031
GGCGTTA2450.016.16287842
TATCAAC38500.015.9996172
TCAACGC38000.015.92067054
GACGAAA2650.015.77374422
TGACGAA2800.015.71444321
CAACGCA38750.015.6693015
ATCAACG39050.015.605263
CTTACAC2850.015.4382263