##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064207_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1468261
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301513150591074	32.0	32.0	32.0	32.0	32.0
2	31.386142518257994	32.0	32.0	32.0	32.0	32.0
3	31.448251366752913	32.0	32.0	32.0	32.0	32.0
4	31.533460331644033	32.0	32.0	32.0	32.0	32.0
5	31.494056574410134	32.0	32.0	32.0	32.0	32.0
6	35.049448973990316	36.0	36.0	36.0	36.0	36.0
7	35.062319982618895	36.0	36.0	36.0	36.0	36.0
8	35.02064891732464	36.0	36.0	36.0	36.0	36.0
9	35.11263188220623	36.0	36.0	36.0	36.0	36.0
10	34.97977607523458	36.0	36.0	36.0	36.0	36.0
11	35.12026540240461	36.0	36.0	36.0	36.0	36.0
12	35.02949475604133	36.0	36.0	36.0	36.0	36.0
13	35.06278175338036	36.0	36.0	36.0	36.0	36.0
14	35.012732068753444	36.0	36.0	36.0	36.0	36.0
15	34.98669378264491	36.0	36.0	36.0	36.0	36.0
16	35.00402925637881	36.0	36.0	36.0	36.0	36.0
17	34.96848584822453	36.0	36.0	36.0	36.0	36.0
18	34.959236811438835	36.0	36.0	36.0	36.0	36.0
19	34.95959437729395	36.0	36.0	36.0	36.0	36.0
20	34.95186959266779	36.0	36.0	36.0	36.0	36.0
21	34.94621187922311	36.0	36.0	36.0	36.0	36.0
22	34.935114397235914	36.0	36.0	36.0	36.0	36.0
23	34.882005311044836	36.0	36.0	36.0	32.0	36.0
24	34.85625716408731	36.0	36.0	36.0	32.0	36.0
25	34.83931603441078	36.0	36.0	36.0	32.0	36.0
26	34.777423087584566	36.0	36.0	36.0	32.0	36.0
27	34.76112285213596	36.0	36.0	36.0	32.0	36.0
28	34.737358684865974	36.0	36.0	36.0	32.0	36.0
29	34.72424861792283	36.0	36.0	36.0	32.0	36.0
30	34.713256702997626	36.0	36.0	36.0	32.0	36.0
31	34.69292448685894	36.0	36.0	36.0	32.0	36.0
32	34.64656964940157	36.0	36.0	36.0	32.0	36.0
33	34.62943577470218	36.0	36.0	36.0	32.0	36.0
34	34.61908747831618	36.0	36.0	36.0	32.0	36.0
35	34.58059636536011	36.0	36.0	36.0	32.0	36.0
36	34.55201016712969	36.0	36.0	36.0	32.0	36.0
37	34.54476554236611	36.0	36.0	36.0	32.0	36.0
38	34.50501988406693	36.0	36.0	36.0	32.0	36.0
39	34.504000310571485	36.0	36.0	36.0	32.0	36.0
40	34.47986359373436	36.0	36.0	36.0	32.0	36.0
41	34.46361035265529	36.0	36.0	36.0	32.0	36.0
42	34.39950730830554	36.0	36.0	36.0	32.0	36.0
43	34.380572663852	36.0	36.0	36.0	32.0	36.0
44	34.33354083504227	36.0	36.0	36.0	32.0	36.0
45	34.29545904985558	36.0	36.0	36.0	32.0	36.0
46	34.268982149631434	36.0	36.0	36.0	32.0	36.0
47	34.23470145975409	36.0	36.0	36.0	32.0	36.0
48	34.18441203573479	36.0	36.0	36.0	32.0	36.0
49	34.16702616224227	36.0	36.0	36.0	32.0	36.0
50	33.579217182776084	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	4.0
20	22.0
21	78.0
22	214.0
23	586.0
24	1436.0
25	3298.0
26	6407.0
27	11290.0
28	18401.0
29	27902.0
30	39188.0
31	54391.0
32	76167.0
33	119580.0
34	244915.0
35	864379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.12502767153799	18.25722099426816	12.014385794028398	26.603365540165452
2	15.749852716319712	20.917483679606065	36.968646454464654	26.36401714960957
3	17.84914745735584	24.935586802020087	29.11496981110229	28.100295929521778
4	12.393504969484308	16.472480029095646	36.30069858151923	34.833316419900825
5	14.07372394962476	37.06800085270943	33.70361264107676	15.154662556589052
6	33.39079359868579	36.07968882916593	17.06992149215977	13.459596079988504
7	29.4131148434201	31.02045959162546	21.439118412270304	18.12730715268413
8	27.648013534378425	33.183541618281765	19.81480131938395	19.35364352795586
9	27.591481791501813	14.224552747739985	18.72373987139731	39.46022558936089
10	15.492111665363522	27.209237701635423	32.319015095583566	24.979635537417487
11	36.54145959063136	21.441351367365886	22.92371724100824	19.09347180099451
12	24.43790609292632	23.819827550978708	29.301826651955377	22.440439704139596
13	29.261830718663116	20.037881728910666	25.44641458494239	25.253872967483826
14	23.541518957512206	19.51402240888346	25.94252913488579	31.00192949871855
15	24.844436210673145	27.040037923781917	22.86624238527511	25.249283480269824
16	25.172756683126167	26.101413247062833	23.992371871275328	24.733458198535672
17	23.750477605820763	25.934489848875643	25.631001572608685	24.68403097269491
18	24.32263082545663	25.571120528191987	26.323510782194358	23.78273786415702
19	25.238632640926923	25.176109697117884	26.032224515940968	23.55303314601423
20	25.491603644051175	24.662524791317043	25.550789508096685	24.2950820565351
21	26.16769089419388	24.562458581955116	25.199198235191155	24.07065228865985
22	25.41595181776512	24.550505053944843	25.501290984764953	24.53225214352509
23	24.427329622347102	24.50265723959018	25.819596486436602	25.250416651626118
24	24.513893760526994	25.08212162923319	25.803388934710664	24.60059567552915
25	24.68617081053101	24.68535350267498	25.929023623602486	24.69945206319152
26	24.456640934162113	25.257767769935878	26.086411630165742	24.199179665736263
27	24.80433137556865	25.1470026311649	25.474553032310272	24.57411296095618
28	24.345031776984214	24.99732666031422	26.054538854012492	24.60310270868907
29	24.394270652418218	25.106624983824062	25.916511718191295	24.582592645566432
30	24.41430500887814	25.280528097762385	25.911226777041207	24.393940116318273
31	24.687006397799696	25.085327835298717	25.397418947921924	24.830246818979663
32	24.511602151995035	25.183300311465796	25.382589382101717	24.922508154437452
33	24.396574858227954	24.902091129149802	25.726841401932433	24.97449261068981
34	24.848099308759057	24.972466829084563	25.808031308467548	24.37140255368883
35	25.25977104186305	24.960087616535986	25.65119846126183	24.12894288033913
36	24.563641639206253	25.300952066989336	25.480086147827162	24.655320145977246
37	25.05256375590257	25.152548976070975	25.454752260735305	24.340135007291156
38	24.567974645521588	25.116763149093785	25.7122773462803	24.602984859104325
39	24.759280890611322	25.117473377355342	25.361512867753483	24.76173286427985
40	25.067070782747873	25.133886155051165	25.5947147065334	24.204328355667556
41	24.318779793912455	25.45111022870691	25.803307562741324	24.42680241463931
42	25.06930075369252	25.45963256638229	25.40371520148641	24.06735147843878
43	24.674985901525904	24.89804146994641	25.607324123238374	24.81964850528931
44	24.509771202723254	25.22661439557348	25.37386522530671	24.88974917639655
45	24.427632210744406	25.44640090230296	25.406557361417693	24.719409525534942
46	24.494652466512786	25.171922707827772	25.4383628912574	24.895061934402047
47	24.56401793443429	25.182923465353348	25.610511109582447	24.64254749062992
48	25.06465280913646	25.561306255776522	24.997905637207328	24.376135297879685
49	24.495183401259727	25.533490072575898	25.20446025761175	24.766866268552622
50	24.454721298970195	25.712008935868795	25.136285620879423	24.69698414428159
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	50.0
2	58.0
3	152.0
4	246.0
5	302.0
6	358.0
7	339.0
8	320.0
9	489.5
10	659.0
11	877.5
12	1096.0
13	2006.0
14	2916.0
15	3891.0
16	4866.0
17	5068.5
18	5271.0
19	5237.0
20	5203.0
21	5705.0
22	6207.0
23	7184.5
24	8162.0
25	9300.0
26	10438.0
27	12831.5
28	15225.0
29	17600.5
30	19976.0
31	22250.0
32	24524.0
33	28187.0
34	31850.0
35	36856.5
36	41863.0
37	48330.5
38	54798.0
39	60778.5
40	66759.0
41	72934.0
42	79109.0
43	82005.5
44	84902.0
45	91936.5
46	98971.0
47	103723.0
48	108475.0
49	111590.5
50	114706.0
51	113295.5
52	111885.0
53	109260.0
54	106635.0
55	105273.0
56	103911.0
57	99672.0
58	95433.0
59	86567.5
60	77702.0
61	67767.5
62	57833.0
63	49957.5
64	42082.0
65	35140.0
66	28198.0
67	23944.5
68	19691.0
69	17313.5
70	14936.0
71	11551.5
72	8167.0
73	6911.5
74	5656.0
75	4472.0
76	3288.0
77	2849.5
78	2411.0
79	1985.0
80	1559.0
81	1198.0
82	837.0
83	699.5
84	562.0
85	402.5
86	243.0
87	164.5
88	86.0
89	66.0
90	46.0
91	38.0
92	30.0
93	24.0
94	18.0
95	21.5
96	25.0
97	23.5
98	22.0
99	13.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00994373616135006
2	4.086466915623312E-4
3	4.086466915623312E-4
4	0.0
5	0.0
6	0.0
7	6.81077819270552E-5
8	0.0
9	0.0012259400746869938
10	0.0011578322927599384
11	0.0
12	6.81077819270552E-5
13	4.767544734893864E-4
14	2.724311277082208E-4
15	0.0036097124421339256
16	7.491856011976073E-4
17	0.0
18	4.767544734893864E-4
19	0.0
20	3.4053890963527605E-4
21	0.0
22	1.362155638541104E-4
23	5.448622554164416E-4
24	8.172933831246624E-4
25	0.0017708023301034354
26	0.005380514772237361
27	0.006129700373434968
28	0.004086466915623312
29	0.002111341239738711
30	0.0032691735324986496
31	0.00681077819270552
32	0.0028605268409363184
33	0.004018359133696257
34	0.0021794490216657664
35	0.003064850186717484
36	0.005925377027653803
37	0.002996742404790429
38	0.008036718267392514
39	0.003814035787915091
40	0.0024518801493739876
41	0.00340538909635276
42	0.0015664789843222698
43	0.0011578322927599384
44	0.0012259400746869938
45	0.0012259400746869938
46	9.535089469787728E-4
47	0.0016345867662493248
48	0.0023156645855198768
49	3.4053890963527605E-4
50	0.0014302634204681592
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1468261.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.87924082831725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.00082886369414	50.932706254135354
2	11.748779019911021	14.775086108633326
3	3.292776084966392	6.211417813209763
4	1.3094174700234071	3.2934070576963084
5	0.6751811857296969	2.12274401901232
6	0.42866040594928295	1.6172304539549518
7	0.2961938864045338	1.3037112700574147
8	0.2266035282176062	1.1398926258672997
9	0.14995606641363782	0.8486211252321241
>10	0.7728533659730419	8.614192604760639
>50	0.05042415885848228	2.183891292435835
>100	0.04505340077377609	5.466181617293402
>500	0.003163477648852304	1.395506154795512
>1k	1.0908543616732084E-4	0.0954116029157315
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.40538909635276E-4
2	0.0	0.0	0.0	0.0	3.40538909635276E-4
3	0.0	0.0	0.0	0.0	3.40538909635276E-4
4	0.0	0.0	0.0	0.0	3.40538909635276E-4
5	0.0	0.0	0.0	0.0	4.086466915623312E-4
6	0.0	0.0	0.0	0.0	4.086466915623312E-4
7	0.0	0.0	0.0	0.0	4.086466915623312E-4
8	0.0	0.0	0.0	0.0	4.086466915623312E-4
9	0.0	0.0	0.0	0.0	4.086466915623312E-4
10	0.0	0.0	0.0	0.0	4.086466915623312E-4
11	0.0	0.0	0.0	0.0	4.086466915623312E-4
12	0.0	0.0	0.0	0.0	6.81077819270552E-4
13	0.0	0.0	0.0	0.0	7.491856011976072E-4
14	0.0	0.0	0.0	0.0	7.491856011976072E-4
15	0.0	0.0	0.0	0.0	0.0010897245108328832
16	0.0	0.0	0.0	0.0	0.0011578322927599384
17	1.362155638541104E-4	0.0	0.0	0.0	0.0011578322927599384
18	1.362155638541104E-4	0.0	0.0	0.0	0.0011578322927599384
19	1.362155638541104E-4	0.0	0.0	0.0	0.0011578322927599384
20	1.362155638541104E-4	0.0	0.0	6.81077819270552E-5	0.0014302634204681592
21	1.362155638541104E-4	0.0	0.0	1.362155638541104E-4	0.0014983712023952144
22	1.362155638541104E-4	0.0	0.0	1.362155638541104E-4	0.0014983712023952144
23	1.362155638541104E-4	6.81077819270552E-5	0.0	3.40538909635276E-4	0.0014983712023952144
24	1.362155638541104E-4	6.81077819270552E-5	0.0	5.448622554164416E-4	0.0014983712023952144
25	1.362155638541104E-4	6.81077819270552E-5	0.0	6.81077819270552E-4	0.0014983712023952144
26	1.362155638541104E-4	6.81077819270552E-5	0.0	6.81077819270552E-4	0.0014983712023952144
27	1.362155638541104E-4	6.81077819270552E-5	0.0	0.0012259400746869936	0.0015664789843222696
28	1.362155638541104E-4	6.81077819270552E-5	0.0	0.00340538909635276	0.0015664789843222696
29	1.362155638541104E-4	6.81077819270552E-5	0.0	0.0066745626288514095	0.0015664789843222696
30	1.362155638541104E-4	6.81077819270552E-5	0.0	0.014234526422754537	0.0015664789843222696
31	1.362155638541104E-4	6.81077819270552E-5	0.0	0.03167011859608067	0.0015664789843222696
32	1.362155638541104E-4	6.81077819270552E-5	0.0	0.0516938064826349	0.0015664789843222696
33	1.362155638541104E-4	6.81077819270552E-5	0.0	0.07008290760293981	0.0015664789843222696
34	1.362155638541104E-4	6.81077819270552E-5	0.0	0.09133253556418103	0.0015664789843222696
35	1.362155638541104E-4	6.81077819270552E-5	0.0	0.11748592382417022	0.0015664789843222696
36	1.362155638541104E-4	6.81077819270552E-5	0.0	0.1538554793732177	0.0015664789843222696
37	1.362155638541104E-4	6.81077819270552E-5	0.0	0.20745630374981014	0.0015664789843222696
38	1.362155638541104E-4	6.81077819270552E-5	0.0	0.2833964805984767	0.0015664789843222696
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2670	0.0	23.15458	1
ACGTAAC	80	8.987199E-5	19.25085	1
GTATTAC	140	4.081812E-9	18.857975	1
ACGATTA	60	0.007413867	18.332893	40
AACGCAG	3375	0.0	17.860312	6
TACCGTC	260	0.0	17.768805	7
ACTGCGC	100	2.4833877E-5	17.600176	8
ATACCGT	230	0.0	17.21698	6
ACCGTCG	220	1.8189894E-12	17.00017	8
CCGTCGT	235	0.0	16.851807	9
CGTCGTA	235	0.0	16.851807	10
GTATTAG	295	0.0	16.407503	1
GGCGTTA	245	0.0	16.162878	42
TATCAAC	3850	0.0	15.999617	2
TCAACGC	3800	0.0	15.9206705	4
GACGAAA	265	0.0	15.773744	22
TGACGAA	280	0.0	15.714443	21
CAACGCA	3875	0.0	15.669301	5
ATCAACG	3905	0.0	15.60526	3
CTTACAC	285	0.0	15.438226	3
>>END_MODULE