FastQCFastQC Report
Thu 2 Feb 2017
SRR4064206_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064206_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643875
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50940.30987757584974523No Hit
GTCCTAAAGTGTGTATTTCTCATTT36620.22276632955668768No Hit
TATCAACGCAGAGTACTTTTTTTTT36020.2191164170025093No Hit
GTCCTACAGTGGACATTTCTAAATT31930.1942361797581933No Hit
CTGTAGGACGTGGAATATGGCAAGA31470.19143791346665653No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31350.19070793095582086No Hit
CTTTAGGACGTGAAATATGGCGAGG31220.1899171165690822No Hit
GTCCTACAGTGTGCATTTCTCATTT24490.14897726408638126No Hit
GATATACACTGTTCTACAAATCCCG23410.14240742148886018No Hit
TTGTAGAACAGTGTATATCAATGAG22550.13717588016120447No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20330.12367120371074443No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17870.10870656223861304No Hit
GGTATCAACGCAGAGTACTTTTTTT17790.10821990723138926No Hit
CTGTAGGACCTGGAATATGGCGAGA16840.10244087902060679No Hit
GTACATGGGGTGGTATCAACGCAAA16640.10122424150254734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCA801.2873534E-714.25109612
TCCAACG3900.013.88822118
TTAGACC551.9650602E-413.8141974
AAAGCGT655.452035E-513.15405710
GTGTTAA655.4645076E-513.1508531
TTAGTAC655.4738783E-513.1484543
CCAACGA2900.013.1072519
AACGGAC1102.733941E-912.95869815
CAACGGA1102.737579E-912.95751514
CGCCAGT2150.012.81728118
AACTATC905.3866097E-712.670341519
CGAGCCG2750.012.4403515
TAGAAAT5500.012.2600994
AGCGTTC701.0901898E-412.21522512
AAATTCG550.003063311312.0936814
GATATAC9150.012.0409771
GAACCGC1501.4551915E-1112.0313286
TCGGCAA951.038914E-612.00092313
GGCGAGG9050.011.97032319
CGCGGTC1353.7107384E-1011.96315410
Produced by FastQC (version 0.11.5)