##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1643875
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289613261348947	32.0	32.0	32.0	32.0	32.0
2	31.418911413580716	32.0	32.0	32.0	32.0	32.0
3	31.48899155957722	32.0	32.0	32.0	32.0	32.0
4	31.56413291764885	32.0	32.0	32.0	32.0	32.0
5	31.524706562238613	32.0	32.0	32.0	32.0	32.0
6	35.09561797581933	36.0	36.0	36.0	36.0	36.0
7	35.116096418523306	36.0	36.0	36.0	36.0	36.0
8	35.064766481636376	36.0	36.0	36.0	36.0	36.0
9	35.172670063113074	36.0	36.0	36.0	36.0	36.0
10	35.03603072009733	36.0	36.0	36.0	36.0	36.0
11	35.17265911337541	36.0	36.0	36.0	36.0	36.0
12	35.08510196943198	36.0	36.0	36.0	36.0	36.0
13	35.122879781005246	36.0	36.0	36.0	36.0	36.0
14	35.077923199756675	36.0	36.0	36.0	36.0	36.0
15	35.043932780777126	36.0	36.0	36.0	36.0	36.0
16	35.0598530910197	36.0	36.0	36.0	36.0	36.0
17	35.03339061668314	36.0	36.0	36.0	36.0	36.0
18	35.02821078245	36.0	36.0	36.0	36.0	36.0
19	35.01550908676146	36.0	36.0	36.0	36.0	36.0
20	35.00191194585963	36.0	36.0	36.0	36.0	36.0
21	34.999769447190324	36.0	36.0	36.0	36.0	36.0
22	34.97382708539275	36.0	36.0	36.0	36.0	36.0
23	34.939483233214204	36.0	36.0	36.0	36.0	36.0
24	34.905658885255875	36.0	36.0	36.0	32.0	36.0
25	34.88271188502775	36.0	36.0	36.0	32.0	36.0
26	34.83121466048209	36.0	36.0	36.0	32.0	36.0
27	34.81504493954832	36.0	36.0	36.0	32.0	36.0
28	34.779039160520114	36.0	36.0	36.0	32.0	36.0
29	34.74454870352064	36.0	36.0	36.0	32.0	36.0
30	34.72049821306364	36.0	36.0	36.0	32.0	36.0
31	34.70682807391073	36.0	36.0	36.0	32.0	36.0
32	34.66245182875827	36.0	36.0	36.0	32.0	36.0
33	34.65585096190404	36.0	36.0	36.0	32.0	36.0
34	34.637989506501405	36.0	36.0	36.0	32.0	36.0
35	34.58770405292373	36.0	36.0	36.0	32.0	36.0
36	34.558980457759866	36.0	36.0	36.0	32.0	36.0
37	34.5237016196487	36.0	36.0	36.0	32.0	36.0
38	34.477369021367196	36.0	36.0	36.0	32.0	36.0
39	34.435601247053455	36.0	36.0	36.0	32.0	36.0
40	34.434667477758346	36.0	36.0	36.0	32.0	36.0
41	34.41079279142271	36.0	36.0	36.0	32.0	36.0
42	34.34915002661395	36.0	36.0	36.0	32.0	36.0
43	34.35438521785416	36.0	36.0	36.0	32.0	36.0
44	34.25971743593643	36.0	36.0	36.0	32.0	36.0
45	34.22830628849517	36.0	36.0	36.0	32.0	36.0
46	34.154165614782144	36.0	36.0	36.0	32.0	36.0
47	34.13460695004182	36.0	36.0	36.0	32.0	36.0
48	34.081516538666264	36.0	36.0	36.0	32.0	36.0
49	34.04299353661319	36.0	36.0	36.0	32.0	36.0
50	33.46849121739792	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	5.0
19	7.0
20	18.0
21	45.0
22	139.0
23	488.0
24	1242.0
25	3041.0
26	6262.0
27	11771.0
28	19841.0
29	30712.0
30	43683.0
31	60669.0
32	87337.0
33	137893.0
34	282115.0
35	958605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93558199537273	19.497266283514413	12.377314320513126	26.189837400599732
2	14.196803883518768	21.589785080328735	40.37004142663349	23.843369609518998
3	17.88340927202273	25.83020555153388	30.238461678843215	26.047923497600173
4	11.197080069956657	17.198874610295796	38.69223633183788	32.911808987909666
5	12.357083111550454	38.80623526728005	35.07590297315794	13.760778648011557
6	31.28081904286835	38.627446209250124	17.692214104521646	12.399520643359876
7	27.693076046628907	32.032340697851964	22.10219402593753	18.172389229581604
8	25.59093605049046	36.49036575165387	20.197581932932856	17.72111626492282
9	26.984955467429266	14.350553242899455	19.691541761945867	38.97294952772541
10	14.914271635880294	27.897959369895766	33.7681851501501	23.419583844073838
11	35.47656328891387	21.449819146722923	25.246034671757084	17.827582892606124
12	24.26171169253043	24.549447340726456	31.13786369968428	20.05097726705883
13	29.052517214046684	20.86441615197392	26.533021952586015	23.550044681393377
14	21.665481457509188	22.115516222968562	26.747382553275102	29.471619766247148
15	23.299430532717448	30.994630180853466	23.129703610560057	22.57623567586903
16	22.931163804194885	26.90413228871819	27.44298629018669	22.721717616900232
17	21.47413884875675	27.825960003041594	27.58427496007908	23.115626188122576
18	21.966936477790767	26.349227978436197	30.231113728247923	21.45272181552511
19	24.628666569741092	25.399407375143944	26.92203108147649	23.049894973638473
20	24.280613942612145	26.735131248452582	27.103470049584306	21.88078475935097
21	24.769462398296707	25.274610295794997	25.98165918941525	23.974268116493043
22	23.99496555088772	27.13705551642036	26.68696019281283	22.18101873987909
23	22.641803265724253	26.7718944777603	27.383989653632725	23.202312602882724
24	23.811539956602434	26.54441863138896	27.470707859679987	22.173333552328618
25	23.332061920830544	26.56418716895369	27.88408367962595	22.21966723058982
26	21.582407313900816	27.58377976534487	28.892536321903133	21.941276598851182
27	22.51645919543097	27.232592066248035	27.560863434000787	22.690085304320213
28	21.816284877372176	27.378900653670318	27.48499660845787	23.319817860499636
29	22.64718532158759	26.050094018295088	27.023674603623615	24.279046056493712
30	22.1076568457054	26.934226063193933	27.740225830805144	23.217891260295524
31	23.634269736746045	25.82330739242798	27.135196892931713	23.40722597789426
32	22.25925666817738	27.549207179640046	27.088632236595966	23.10290391558661
33	22.0899058089643	27.03829632428046	28.144279033719087	22.727518833036154
34	22.88026143933988	26.95332770012343	28.363151020517414	21.803259840019273
35	23.045074223364818	27.367805029985	27.60882869313101	21.978292053519176
36	21.857256621609043	27.181920332021896	27.780430930064053	23.180392116305004
37	22.83267692824318	27.10861770456539	26.513777206743878	23.544928160447547
38	22.732501936134632	27.717539098360504	26.90986829417566	22.640090671329208
39	23.583901885575756	26.2948847027762	26.683740977433317	23.43747243421473
40	24.439462838197496	26.639332045686125	27.386431037367114	21.53477407874926
41	23.1759189300006	25.978425782965715	28.63413987227265	22.21151541476104
42	23.47570638516097	28.157038423143067	27.104129570812503	21.263125620883464
43	21.869804501746202	27.469217100576632	28.02541106219481	22.63556733548235
44	21.702913825019635	27.724741810855352	27.063368804874887	23.50897555925013
45	21.947360352731625	27.87306187403276	27.321553712733966	22.85802406050165
46	22.636701367576375	26.30678945918045	26.828671837027183	24.227837336215984
47	23.495227017780362	26.72359043116223	26.98079370448147	22.800388846575935
48	22.882864906922038	27.18231795488239	26.198157116551098	23.736660021644482
49	22.943105929816493	26.984564397741416	26.864602989788683	23.20772668265341
50	22.224466269835236	28.298177150661097	26.223663279303832	23.253693300199835
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	157.5
2	66.0
3	208.5
4	351.0
5	409.0
6	467.0
7	433.0
8	399.0
9	507.0
10	615.0
11	1006.0
12	1397.0
13	2479.0
14	3561.0
15	4684.5
16	5808.0
17	6549.0
18	7290.0
19	7382.5
20	7475.0
21	8572.0
22	9669.0
23	10952.5
24	12236.0
25	15386.0
26	18536.0
27	25561.5
28	32587.0
29	38839.5
30	45092.0
31	50374.5
32	55657.0
33	65966.0
34	76275.0
35	89014.5
36	101754.0
37	106072.0
38	110390.0
39	106758.0
40	103126.0
41	101407.0
42	99688.0
43	93751.0
44	87814.0
45	91371.0
46	94928.0
47	96993.0
48	99058.0
49	98622.5
50	98187.0
51	94568.0
52	90949.0
53	87921.5
54	84894.0
55	84888.0
56	84882.0
57	82578.0
58	80274.0
59	72002.0
60	63730.0
61	56261.0
62	48792.0
63	42463.0
64	36134.0
65	30343.0
66	24552.0
67	21566.5
68	18581.0
69	16884.5
70	15188.0
71	11708.0
72	8228.0
73	6984.0
74	5740.0
75	4394.0
76	3048.0
77	2732.5
78	2417.0
79	2012.0
80	1607.0
81	1277.5
82	948.0
83	804.0
84	660.0
85	467.0
86	274.0
87	196.0
88	118.0
89	93.5
90	69.0
91	54.0
92	39.0
93	28.5
94	18.0
95	24.5
96	31.0
97	22.0
98	13.0
99	13.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008638126378222187
2	3.041593795148658E-4
3	3.041593795148658E-4
4	0.0
5	0.0
6	3.041593795148658E-4
7	1.216637518059463E-4
8	0.0
9	0.0013383012698654094
10	0.0012166375180594632
11	6.083187590297315E-5
12	3.041593795148658E-4
13	8.516462626416241E-4
14	1.216637518059463E-4
15	0.003528248802372443
16	9.733100144475704E-4
17	0.0
18	5.474868831267585E-4
19	1.216637518059463E-4
20	4.866550072237852E-4
21	0.0
22	6.083187590297315E-5
23	7.908143867386511E-4
24	2.433275036118926E-4
25	0.0020682837807010873
26	0.006144019466200289
27	0.006874001977035967
28	0.004866550072237853
29	0.0027982662915367654
30	0.004744886320431906
31	0.00845563075051327
32	0.0036499125541783898
33	0.0053532050794616376
34	0.0031632575469546045
35	0.0032240894228575774
36	0.0077864801155805635
37	0.00444072694091704
38	0.00827313512280435
39	0.004866550072237853
40	0.003041593795148658
41	0.003710744430081362
42	0.0029199300433427116
43	0.0014599650216713558
44	0.0013383012698654094
45	0.0011558056421564899
46	0.001094973766253517
47	0.002372443160215953
48	0.0023116112843129797
49	7.908143867386511E-4
50	0.0012166375180594632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1643875.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.55318880140365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.61292174315913	38.224743087076824
2	14.142540786413694	14.299010690142461
3	4.5392718643174055	6.884240027332118
4	1.967722264991456	3.9789854068335475
5	1.02514229042181	2.5912105877998566
6	0.5864810510319525	1.7789092380758365
7	0.38155339988997866	1.350211874371889
8	0.251157912159117	1.0157466681876952
9	0.19572905519157666	0.8905255092918116
>10	1.0733571102310582	10.068578145953884
>50	0.1117493443418536	3.9567842854017288
>100	0.10014310328846265	10.23508989250694
>500	0.009808277619357778	3.2386903152177857
>1k	0.0024217969430513028	1.4872742718077252
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.083187590297316E-5	0.0
6	0.0	0.0	0.0	6.083187590297316E-5	0.0
7	0.0	0.0	0.0	6.083187590297316E-5	0.0
8	0.0	0.0	0.0	6.083187590297316E-5	0.0
9	0.0	0.0	0.0	1.2166375180594632E-4	0.0
10	0.0	0.0	0.0	1.2166375180594632E-4	0.0
11	0.0	0.0	0.0	1.2166375180594632E-4	0.0
12	0.0	0.0	0.0	1.2166375180594632E-4	0.0
13	0.0	0.0	0.0	1.2166375180594632E-4	0.0
14	0.0	0.0	0.0	1.2166375180594632E-4	0.0
15	0.0	0.0	0.0	1.8249562770891948E-4	0.0
16	0.0	0.0	0.0	1.8249562770891948E-4	0.0
17	0.0	0.0	0.0	1.8249562770891948E-4	0.0
18	0.0	0.0	0.0	1.8249562770891948E-4	0.0
19	0.0	0.0	0.0	2.4332750361189264E-4	6.083187590297316E-5
20	0.0	0.0	0.0	3.041593795148658E-4	6.083187590297316E-5
21	0.0	0.0	0.0	4.258231313208121E-4	6.083187590297316E-5
22	0.0	0.0	0.0	6.691506349327047E-4	6.083187590297316E-5
23	0.0	0.0	0.0	0.0014599650216713558	1.2166375180594632E-4
24	0.0	0.0	0.0	0.0024332750361189264	1.2166375180594632E-4
25	0.0	0.0	0.0	0.002980761919245685	1.2166375180594632E-4
26	0.0	0.0	0.0	0.004136567561402175	1.2166375180594632E-4
27	0.0	0.0	0.0	0.005414036955364611	1.2166375180594632E-4
28	0.0	0.0	0.0	0.010098091399893544	1.2166375180594632E-4
29	0.0	0.0	0.0	0.018918713405824652	1.2166375180594632E-4
30	0.0	0.0	0.0	0.03516082427191849	1.2166375180594632E-4
31	0.0	0.0	0.0	0.07470154360885103	1.2166375180594632E-4
32	0.0	0.0	0.0	0.11679720173370846	1.2166375180594632E-4
33	0.0	0.0	0.0	0.15901452361037183	1.2166375180594632E-4
34	0.0	0.0	0.0	0.20457759866169872	1.2166375180594632E-4
35	0.0	0.0	0.0	0.2547638962816516	1.2166375180594632E-4
36	0.0	0.0	0.0	0.32441639419055585	1.2166375180594632E-4
37	0.0	0.0	0.0	0.4334879476845867	1.2166375180594632E-4
38	0.0	0.0	0.0	0.5749828910349023	1.2166375180594632E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	1355	0.0	20.943506	1
ACCGTCG	305	0.0	20.91689	8
TTAGGAC	1465	0.0	20.871946	3
CCGTAGA	65	4.929959E-4	20.309671	35
GTACGTT	55	0.0044833357	20.000732	40
TAGGACG	2470	0.0	19.593475	4
GGACCGA	90	1.00305515E-5	19.553894	6
TGTAGGA	2595	0.0	19.496784	2
CGTCGTA	350	0.0	19.48465	10
GTATCAA	4265	0.0	19.187698	1
TACCGTC	335	0.0	19.043156	7
AGGACGT	2520	0.0	18.942833	5
TAGGACC	1410	0.0	18.877827	4
CTGTAGG	2655	0.0	18.477333	1
GGACGTG	2445	0.0	18.444239	6
TATACGG	60	0.0074077407	18.335678	36
ATACCGT	360	0.0	18.331776	6
TAGGACA	855	0.0	18.267452	4
CCGTCGT	350	0.0	18.22813	9
GTCGTAG	375	0.0	18.185675	11
>>END_MODULE