FastQCFastQC Report
Thu 2 Feb 2017
SRR4064205_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064205_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916249
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41260.21531648548805504No Hit
TATCAACGCAGAGTACTTTTTTTTT29590.15441625801239817No Hit
GCGCAAGACGGACCAGAGCGAAAGC25230.13166347379698567No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20900.10906724543626638No Hit
GTATCTGATCGTCTTCGAACCTCCG20860.10885850429667543No Hit
GAATAGGACCGCGGTTCTATTTTGT20540.10718857517994791No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20090.10484023735954982No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19340.1009263409922197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA400.005277817614.2507649
AACGGAC1400.013.57321915
GTACTAA500.001500343813.2993231
CGAACGA1153.765308E-1013.2187916
AACCGCG1450.013.1038087
CGGTCCA3600.012.93158710
TCCAACG2500.012.54132718
CGCCGGT3750.012.4136757
GCGAGTC701.0906093E-412.21494113
CCGGTCC3900.012.18014059
TATACCG550.00307246512.0890315
GTTTTCG2250.011.82378315
CAACGGA1701.8189894E-1211.73684314
CGGTTCT3750.011.65395812
CGTTATT1552.5465852E-1111.6409212
GCCGGTC4000.011.6381248
TAGCTCG752.0727576E-411.40090910
AAGACGG3850.011.3488865
TTTTCGG2350.011.32034816
CGGCGTC2100.011.31101614
Produced by FastQC (version 0.11.5)