FastQCFastQC Report
Thu 2 Feb 2017
SRR4064205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916249
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21990.1147554414901195No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21000.10958909828524373No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20740.10823228087790261No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19790.10327467881261777No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19350.10097852627711744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG500.00258284221.9972744
GTATCAA33100.021.6666851
GATATAC10150.019.7232381
CGCAATA6100.019.47909536
ATACGAA6350.019.05424740
ATACACT10850.018.2465884
ACGTATG2300.018.17735332
CGTCGTA4400.017.99917810
GTAGAAC12600.017.8073183
CAATACG6850.017.66711438
TCTAGCG7000.017.60103428
ACCGTCG4250.017.5982788
CGGTCCA6900.017.5354310
TTAGGAC4400.017.4978313
AACGCAG40750.017.4358756
CTAGCGG6950.017.41109529
AATACGA6950.017.41063939
AATCCCG6850.017.02067619
TACGAAT7250.016.99188241
TACCGTC4550.016.9209797
Produced by FastQC (version 0.11.5)