##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1916249
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265014097854714	32.0	32.0	32.0	32.0	32.0
2	31.24271115079512	32.0	32.0	32.0	32.0	32.0
3	31.375211676561868	32.0	32.0	32.0	32.0	32.0
4	31.485185380396807	32.0	32.0	32.0	32.0	32.0
5	31.40235741805997	32.0	32.0	32.0	32.0	32.0
6	34.981290531658466	36.0	36.0	36.0	36.0	36.0
7	35.00382048470736	36.0	36.0	36.0	36.0	36.0
8	34.936047977063524	36.0	36.0	36.0	36.0	36.0
9	35.09287832635529	36.0	36.0	36.0	36.0	36.0
10	34.90234306710662	36.0	36.0	36.0	32.0	36.0
11	35.08668915156642	36.0	36.0	36.0	36.0	36.0
12	34.97503090673498	36.0	36.0	36.0	36.0	36.0
13	35.03205168013134	36.0	36.0	36.0	36.0	36.0
14	34.966638991070575	36.0	36.0	36.0	32.0	36.0
15	34.923856450805715	36.0	36.0	36.0	32.0	36.0
16	34.949127175017445	36.0	36.0	36.0	36.0	36.0
17	34.90594228620602	36.0	36.0	36.0	32.0	36.0
18	34.903220301745755	36.0	36.0	36.0	32.0	36.0
19	34.907752593739126	36.0	36.0	36.0	32.0	36.0
20	34.894280440589924	36.0	36.0	36.0	32.0	36.0
21	34.89519577048703	36.0	36.0	36.0	32.0	36.0
22	34.8781176141514	36.0	36.0	36.0	32.0	36.0
23	34.82808601596139	36.0	36.0	36.0	32.0	36.0
24	34.79348978133844	36.0	36.0	36.0	32.0	36.0
25	34.761623358968485	36.0	36.0	36.0	32.0	36.0
26	34.69713069648047	36.0	36.0	36.0	32.0	36.0
27	34.70468242905802	36.0	36.0	36.0	32.0	36.0
28	34.67277060549021	36.0	36.0	36.0	32.0	36.0
29	34.63966008592829	36.0	36.0	36.0	32.0	36.0
30	34.62345342385045	36.0	36.0	36.0	32.0	36.0
31	34.61775479073962	36.0	36.0	36.0	32.0	36.0
32	34.573538720698615	36.0	36.0	36.0	32.0	36.0
33	34.551229511404834	36.0	36.0	36.0	32.0	36.0
34	34.54382441947784	36.0	36.0	36.0	32.0	36.0
35	34.49875668558731	36.0	36.0	36.0	32.0	36.0
36	34.46715693002319	36.0	36.0	36.0	32.0	36.0
37	34.466240034567534	36.0	36.0	36.0	32.0	36.0
38	34.41608214798808	36.0	36.0	36.0	32.0	36.0
39	34.41409858530911	36.0	36.0	36.0	32.0	36.0
40	34.399614037632894	36.0	36.0	36.0	32.0	36.0
41	34.37632061386594	36.0	36.0	36.0	32.0	36.0
42	34.29976741018521	36.0	36.0	36.0	32.0	36.0
43	34.308155151026824	36.0	36.0	36.0	32.0	36.0
44	34.226864045330224	36.0	36.0	36.0	32.0	36.0
45	34.19777492382253	36.0	36.0	36.0	32.0	36.0
46	34.17080295932313	36.0	36.0	36.0	32.0	36.0
47	34.14671945034283	36.0	36.0	36.0	32.0	36.0
48	34.093274934520515	36.0	36.0	36.0	32.0	36.0
49	34.0715684652673	36.0	36.0	36.0	32.0	36.0
50	33.43321092405006	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	12.0
21	52.0
22	170.0
23	507.0
24	1530.0
25	3318.0
26	7058.0
27	12998.0
28	21970.0
29	35333.0
30	52244.0
31	76983.0
32	113915.0
33	185946.0
34	380369.0
35	1023838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35135975817243	18.459497431208366	11.947490600581604	26.241652210037596
2	15.697769687835619	20.512839246146346	37.557260752079344	26.232130313938697
3	18.163491620330387	24.39025281214041	29.29948936592137	28.146766201607832
4	11.777696948569837	16.11507690284509	36.778779793231465	35.32844635535361
5	13.688513340385306	37.40975207292998	33.99370332352424	14.908031263160476
6	34.09651072437055	36.73516354658569	16.401938585033317	12.76638714401044
7	29.678345559475833	30.9324623261382	20.91173954950531	18.47745256488066
8	27.60377174365127	34.09066358286423	19.18571125151272	19.11985342197178
9	26.98413858102036	13.799601507549752	18.524106288311064	40.69215362311883
10	15.102704107485884	27.39171071576369	32.33056731618754	25.17501786056289
11	37.650711181433714	20.95631671892156	22.68224154702314	18.710730552621584
12	24.057637633542214	23.967409228868082	29.613599074858787	22.36135406273092
13	29.258108857041766	19.595426037892995	25.74497531099999	25.401489794065245
14	22.973222616744003	20.078132012415956	25.53620520142529	31.41244016941475
15	24.807623560601513	27.97368702590852	22.461635610712023	24.757053802777946
16	25.306800011689607	26.140102033574227	24.380189872625028	24.172908082111142
17	23.367526871507827	26.420157296885737	25.685492856095426	24.526822975511013
18	24.194987694677714	25.58557260975116	26.76131014630705	23.458129549264083
19	25.17555139000797	25.29688224071206	25.652629513507648	23.874936855772322
20	25.199467078864707	25.074377775957075	25.326382883900738	24.399772261277477
21	26.544410460227247	24.533033024413843	24.82297446730566	24.099582048053254
22	25.26243993470574	24.830449920887066	25.514964660106624	24.39214548430057
23	24.202772624443245	24.572768997539445	25.940711864672128	25.28374651334518
24	24.51938306105462	25.477981562804146	25.659431124053157	24.34320425208808
25	24.683191221880172	24.841889157763283	25.91285234099025	24.562067279366293
26	23.976582245781	25.965463091934726	26.414905281571983	23.64304938071229
27	24.648862367825966	25.255502108678485	25.63981861346496	24.455816910030588
28	23.8873377623071	25.404009038863983	26.25696049932939	24.45169269949952
29	24.03767673035535	24.995929435193474	26.053075990138797	24.913317844312378
30	23.943600229206265	25.590524471108633	26.206124900713817	24.259750398971285
31	24.580870011773843	25.15134814666366	25.36104361331697	24.90673822824553
32	24.165230220223812	25.55142060769643	25.20265506445874	25.08069410762102
33	24.029080866351386	25.051795922499732	25.93282238460599	24.98630082654289
34	24.778622974363685	25.19961506908508	26.130264880063958	23.891497076487276
35	25.10693127591087	25.22304747352029	25.955440604868844	23.714580645699996
36	23.970012238305113	25.686506290071314	25.57591793811958	24.767563533503992
37	24.90301045931867	25.45520111304435	25.39085454126061	24.250933886376373
38	24.232411790831268	25.360015322799086	25.732073342765695	24.67549954360396
39	24.88782287129612	25.08263897804261	25.219265971737315	24.810272178923956
40	25.194629387445815	25.4283725236222	25.743109039424777	23.633889049507204
41	24.115301718514342	25.3948721160219	26.50645290915828	23.98337325630548
42	25.307429449351115	26.046961400930584	25.395415538572973	23.250193611145324
43	24.33747376353311	25.152878627051294	26.001943403335513	24.507704206080074
44	24.04525775692195	25.7216275341505	25.40501528005077	24.82809942887679
45	24.112604671891518	25.958263795606573	25.599224726102243	24.329906806399663
46	24.155253167034143	25.350728646166292	25.236597998669257	25.257420188130308
47	24.488207960722427	25.441392583686113	25.667725006888602	24.402674448702854
48	25.176676877795227	25.704176473351044	24.752454062968045	24.36669258588568
49	24.232064634842573	25.982835153388766	25.20720243059071	24.577897781177953
50	24.444650868607948	26.034806940931322	24.903938833969043	24.61660335649169
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	24.5
2	26.0
3	105.0
4	184.0
5	218.5
6	253.0
7	246.5
8	240.0
9	355.0
10	470.0
11	678.5
12	887.0
13	1732.0
14	2577.0
15	3519.5
16	4462.0
17	4985.5
18	5509.0
19	5788.0
20	6067.0
21	7045.0
22	8023.0
23	8967.5
24	9912.0
25	12496.0
26	15080.0
27	19867.5
28	24655.0
29	28440.0
30	32225.0
31	34526.0
32	36827.0
33	42448.5
34	48070.0
35	55760.0
36	63450.0
37	72676.0
38	81902.0
39	87149.0
40	92396.0
41	98941.0
42	105486.0
43	106004.0
44	106522.0
45	115517.0
46	124512.0
47	130894.0
48	137276.0
49	141211.5
50	145147.0
51	139836.0
52	134525.0
53	131957.5
54	129390.0
55	129770.5
56	130151.0
57	125519.5
58	120888.0
59	109902.0
60	98916.0
61	86786.0
62	74656.0
63	64715.5
64	54775.0
65	46533.5
66	38292.0
67	32706.5
68	27121.0
69	24608.0
70	22095.0
71	17006.5
72	11918.0
73	10092.0
74	8266.0
75	6367.5
76	4469.0
77	3945.0
78	3421.0
79	2782.5
80	2144.0
81	1734.0
82	1324.0
83	1102.5
84	881.0
85	639.0
86	397.0
87	278.0
88	159.0
89	125.5
90	92.0
91	80.0
92	68.0
93	60.0
94	52.0
95	45.0
96	38.0
97	24.5
98	11.0
99	15.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009028054287308173
2	7.827792734660266E-4
3	2.0874113959094042E-4
4	0.0
5	0.0
6	3.1311170938641065E-4
7	0.0
8	0.0
9	0.0014090026922388478
10	8.349645583637617E-4
11	5.2185284897735106E-5
12	5.2185284897735106E-5
13	5.740381338750862E-4
14	2.609264244886755E-4
15	0.004383563931409749
16	8.871498432614969E-4
17	0.0
18	2.609264244886755E-4
19	5.2185284897735106E-5
20	9.393351281592318E-4
21	0.0
22	5.2185284897735106E-5
23	7.305939885682914E-4
24	7.305939885682914E-4
25	0.0015655585469320531
26	0.00500978735018257
27	0.0062622341877282125
28	0.004122637506921074
29	0.0027658200995799606
30	0.0037051552277391924
31	0.007149384030989709
32	0.003235487663659577
33	0.003913896367330133
34	0.003183302378761841
35	0.0033920435183527817
36	0.006992828176296504
37	0.003287672948557312
38	0.007827792734660267
39	0.0043313786465120135
40	0.0022439672506026098
41	0.0030789318089663714
42	0.001930855541216199
43	0.0012002615526479075
44	0.0013046321224433777
45	0.0012002615526479075
46	0.0012524468375456426
47	0.0021395966808071395
48	0.002035226111011669
49	3.652969942841457E-4
50	0.0011480762677501723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1916249.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.7580663224921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99675915572779	37.559324088759055
2	14.683121551240733	14.905737150381803
3	5.123885059555741	7.8023549304527
4	2.2683728883018004	4.60552886034263
5	1.143043011386972	2.9009326490720513
6	0.689607801980165	2.100189512965033
7	0.42943732417286706	1.5258185727203948
8	0.29647939701666337	1.203897671761941
9	0.2051341232109886	0.9370990287854661
>10	0.9920524055394665	8.930928368392179
>50	0.08109975573547654	2.9101427911404003
>100	0.07749172801340674	8.480278041785654
>500	0.009698358954889604	3.389330954593718
>1k	0.0038174391630948438	2.7484373788471497
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2199	0.1147554414901195	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2100	0.10958909828524373	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2074	0.10823228087790261	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1979	0.10327467881261777	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1935	0.10097852627711744	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.2185284897735106E-5	0.0
5	0.0	0.0	0.0	5.2185284897735106E-5	0.0
6	0.0	0.0	0.0	5.2185284897735106E-5	0.0
7	0.0	0.0	0.0	5.2185284897735106E-5	0.0
8	0.0	0.0	0.0	5.2185284897735106E-5	0.0
9	0.0	0.0	0.0	1.0437056979547021E-4	0.0
10	0.0	0.0	0.0	1.0437056979547021E-4	0.0
11	0.0	0.0	0.0	1.0437056979547021E-4	0.0
12	0.0	0.0	0.0	1.0437056979547021E-4	0.0
13	0.0	0.0	0.0	1.5655585469320532E-4	0.0
14	0.0	0.0	0.0	2.0874113959094042E-4	0.0
15	0.0	0.0	0.0	2.0874113959094042E-4	0.0
16	0.0	0.0	0.0	2.0874113959094042E-4	0.0
17	0.0	0.0	0.0	2.0874113959094042E-4	0.0
18	0.0	0.0	0.0	2.0874113959094042E-4	0.0
19	0.0	0.0	0.0	2.0874113959094042E-4	0.0
20	0.0	0.0	0.0	2.609264244886755E-4	0.0
21	0.0	0.0	0.0	3.1311170938641065E-4	0.0
22	0.0	0.0	0.0	4.1748227918188085E-4	0.0
23	0.0	0.0	0.0	5.740381338750862E-4	0.0
24	0.0	0.0	0.0	7.305939885682914E-4	0.0
25	0.0	0.0	0.0	7.827792734660266E-4	0.0
26	0.0	0.0	0.0	7.827792734660266E-4	0.0
27	0.0	0.0	0.0	8.871498432614968E-4	0.0
28	0.0	0.0	0.0	0.0016177438318297883	0.0
29	0.0	0.0	0.0	0.003966081652227868	0.0
30	0.0	0.0	0.0	0.007932163304455736	0.0
31	0.0	0.0	0.0	0.017325514586048054	0.0
32	0.0	0.0	0.0	0.02974561239170901	0.0
33	0.0	0.0	0.0	0.04190478377288129	0.0
34	0.0	0.0	0.0	0.05672540468383806	0.0
35	0.0	0.0	0.0	0.0743118456943748	0.0
36	0.0	0.0	0.0	0.10076978513752649	0.0
37	0.0	0.0	0.0	0.14215271606143043	0.0
38	0.0	0.0	0.0	0.19804315618690474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	50	0.002582842	21.997274	4
GTATCAA	3310	0.0	21.666685	1
GATATAC	1015	0.0	19.723238	1
CGCAATA	610	0.0	19.479095	36
ATACGAA	635	0.0	19.054247	40
ATACACT	1085	0.0	18.246588	4
ACGTATG	230	0.0	18.177353	32
CGTCGTA	440	0.0	17.999178	10
GTAGAAC	1260	0.0	17.807318	3
CAATACG	685	0.0	17.667114	38
TCTAGCG	700	0.0	17.601034	28
ACCGTCG	425	0.0	17.598278	8
CGGTCCA	690	0.0	17.53543	10
TTAGGAC	440	0.0	17.497831	3
AACGCAG	4075	0.0	17.435875	6
CTAGCGG	695	0.0	17.411095	29
AATACGA	695	0.0	17.410639	39
AATCCCG	685	0.0	17.020676	19
TACGAAT	725	0.0	16.991882	41
TACCGTC	455	0.0	16.920979	7
>>END_MODULE