FastQCFastQC Report
Thu 2 Feb 2017
SRR4064204_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064204_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2308092
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC27230.11797623318307936No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG26480.11472679598560195No Hit
GTATCAACGCAGAGTACTTTTTTTT25690.11130405547092577No Hit
GAATAGGACCGCGGTTCTATTTTGT25200.10918108983524054No Hit
GTCTTGCGCCGGTCCAAGAATTTCA24320.10536841685686706No Hit
GATTAAGAGGGACGGCCGGGGGCAT23980.10389533866067731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC400.005287167614.2471391
GGTATCA7000.014.1114521
TACGGTG500.001500976713.2987715
GTATCAA20250.012.7110271
CGACTAA604.0913126E-412.66741813
CGGTCCA3850.012.33812610
CGAAATT1251.4097168E-912.16072213
ACCGTTA1103.8149665E-812.0913658
TCGCGTA1650.012.0913649
CGAACGA1353.7107384E-1011.96471116
CGTCGTA3600.011.87544710
AAGACGG5050.011.85038955
CAAGACG5250.011.7597984
GTCCTAA2100.011.7595431
TACCGTC3800.011.75044257
GCGAAAT1302.6138878E-911.69325412
GCCGGTC3900.011.6927498
CGCCGGT3700.011.5544897
ACCGTCG3800.011.5004338
CGCAAGA4850.011.3565742
Produced by FastQC (version 0.11.5)