##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064204_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2308092
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230781961897534	32.0	32.0	32.0	32.0	32.0
2	30.802164298476836	32.0	32.0	32.0	32.0	32.0
3	30.817833517901366	32.0	32.0	32.0	32.0	32.0
4	30.860105229774202	32.0	32.0	32.0	32.0	32.0
5	30.74857587999092	32.0	32.0	32.0	32.0	32.0
6	34.39309524923617	36.0	36.0	36.0	32.0	36.0
7	34.339545823996616	36.0	36.0	36.0	32.0	36.0
8	34.2987263939219	36.0	36.0	36.0	32.0	36.0
9	34.4551893945302	36.0	36.0	36.0	32.0	36.0
10	34.12980028525726	36.0	36.0	36.0	32.0	36.0
11	34.41812111475626	36.0	36.0	36.0	32.0	36.0
12	34.22419426955251	36.0	36.0	36.0	32.0	36.0
13	34.31853582959432	36.0	36.0	36.0	32.0	36.0
14	34.2068782353563	36.0	36.0	36.0	32.0	36.0
15	34.130584916025875	36.0	36.0	36.0	32.0	36.0
16	34.14348474844157	36.0	36.0	36.0	32.0	36.0
17	34.06072764863792	36.0	36.0	36.0	32.0	36.0
18	34.053579320061765	36.0	36.0	36.0	32.0	36.0
19	34.080366813801184	36.0	36.0	36.0	32.0	36.0
20	34.06936205315906	36.0	36.0	36.0	32.0	36.0
21	34.05388953299955	36.0	36.0	36.0	32.0	36.0
22	34.02643785429697	36.0	36.0	36.0	32.0	36.0
23	33.968658961601186	36.0	36.0	36.0	32.0	36.0
24	33.95061895279738	36.0	36.0	36.0	32.0	36.0
25	33.534446200584725	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	15.0
5	25.0
6	85.0
7	32.0
8	131.0
9	145.0
10	171.0
11	63.0
12	123.0
13	104.0
14	174.0
15	321.0
16	559.0
17	753.0
18	961.0
19	1413.0
20	2077.0
21	3171.0
22	5204.0
23	8180.0
24	12459.0
25	18326.0
26	26374.0
27	35562.0
28	48944.0
29	66187.0
30	87354.0
31	118342.0
32	166089.0
33	232818.0
34	483034.0
35	988886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.361857346129526	16.949882739140858	11.180587790914636	26.507672123814984
2	17.154389855323014	19.03344455666432	36.774540732566166	27.037624855446495
3	18.85363415753237	22.605011397022345	28.57591595362164	29.965438491823647
4	12.677825428007186	15.02684983072085	35.59987729753009	36.69544744374187
5	14.915190637996222	35.940009064588224	33.775860290950746	15.3689400064648
6	35.19490980847218	35.34019900243131	16.173724798087886	13.291166391008622
7	30.923797286785593	30.063464342746453	20.36059187162392	18.652146498844036
8	28.42496967269919	32.967195373694324	18.826633589430543	19.78120136417595
9	27.121175014683345	13.98890784058326	18.23695782682101	40.652959317912384
10	15.82089500478575	26.44938027993175	31.434104024192315	26.295620691090182
11	37.91989554872681	20.783141800319303	22.061430144959953	19.23553250599394
12	24.472418907126045	23.425515247493507	28.659903490126837	23.442162355253615
13	29.392197554327915	19.00873417156294	25.1251253421032	26.473942932005944
14	23.530378287641152	19.42967904272508	24.50678066842941	32.53316200120435
15	25.371510961524123	27.0003936195485	21.797115999854345	25.830979419073035
16	26.281412939007296	25.65336008493692	23.034153815073182	25.031073160982604
17	24.412063577941243	25.809810674241085	24.694673630691653	25.083452117126022
18	25.23097734776567	24.925348866954707	25.39916189551226	24.444511889767366
19	25.950244097014476	24.779532270232306	24.73262055270848	24.537603080044743
20	25.723783706910307	24.332842514932672	24.331324966874092	25.61204881128293
21	27.090551281812264	23.915904062544303	24.00734285123399	24.98620180440944
22	26.112321044619446	24.01890242289727	24.54956292808052	25.319213604402762
23	24.761694156823978	23.945661411450477	25.20760705551798	26.08503737620757
24	25.113269309978538	24.833011771336903	24.54677101172798	25.50694790695658
25	25.083062888459644	24.314067252431837	24.75107148199455	25.851798377113965
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	69.0
2	187.0
3	305.0
4	305.0
5	305.0
6	967.0
7	1629.0
8	1629.0
9	1629.0
10	2147.5
11	2666.0
12	2666.0
13	2666.0
14	3921.5
15	5177.0
16	5177.0
17	5177.0
18	8813.0
19	12449.0
20	12449.0
21	12449.0
22	20062.0
23	27675.0
24	27675.0
25	27675.0
26	41364.0
27	55053.0
28	55053.0
29	55053.0
30	70436.0
31	85819.0
32	85819.0
33	85819.0
34	110420.5
35	135022.0
36	135022.0
37	135022.0
38	158788.5
39	182555.0
40	182555.0
41	182555.0
42	213790.5
43	245026.0
44	245026.0
45	245026.0
46	271422.0
47	297818.0
48	297818.0
49	297818.0
50	308573.0
51	319328.0
52	319328.0
53	319328.0
54	301498.0
55	283668.0
56	283668.0
57	283668.0
58	261697.0
59	239726.0
60	239726.0
61	239726.0
62	209680.0
63	179634.0
64	179634.0
65	179634.0
66	147258.5
67	114883.0
68	114883.0
69	114883.0
70	85976.0
71	57069.0
72	57069.0
73	57069.0
74	43814.0
75	30559.0
76	30559.0
77	30559.0
78	24425.5
79	18292.0
80	18292.0
81	18292.0
82	12484.0
83	6676.0
84	6676.0
85	6676.0
86	5143.5
87	3611.0
88	3611.0
89	3611.0
90	2551.5
91	1492.0
92	1492.0
93	1492.0
94	1056.0
95	620.0
96	620.0
97	620.0
98	945.5
99	1271.0
100	1271.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03652367409964594
2	0.005329077003862931
3	0.0014297523668900546
4	0.0030328080509789035
5	0.009098424152936711
6	0.012174558033215314
7	0.02153293716195022
8	0.03314425941426945
9	0.045795401569781444
10	0.053897331648825084
11	0.05108115274434468
12	0.06000627358008259
13	0.060872790166076565
14	0.06329903660685969
15	0.05615027477240942
16	0.06234586836226633
17	0.0597896444335841
18	0.07352393232158856
19	0.07070775341710815
20	0.07538694298147561
21	0.07066442758780846
22	0.07456375222478133
23	0.0785064026910539
24	0.07114101171010515
25	0.07395719061458556
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2308092.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.52010630588695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32643763034392	38.78733518016054
2	12.203685195656739	12.08655576425001
3	3.7985126333902963	5.643082482292261
4	1.676337573507201	3.3204965937851667
5	0.9312195444074314	2.3057045416587805
6	0.6137373785758364	1.823540413858308
7	0.418195505113512	1.4496360108905642
8	0.29017812338911875	1.149572121429755
9	0.22896038212867179	1.0204328216563492
>10	1.308656159333429	11.961723854791144
>50	0.1036752223552057	3.590563581539838
>100	0.08310416744289839	8.707234866921784
>500	0.012206940738392891	4.202894619374293
>1k	0.005093543617458876	3.951227147391339
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	2723	0.11797623318307936	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2648	0.11472679598560195	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2569	0.11130405547092577	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2520	0.10918108983524054	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2432	0.10536841685686706	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2398	0.10389533866067731	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2997748789909589E-4
2	0.0	0.0	0.0	0.0	1.2997748789909589E-4
3	0.0	0.0	0.0	0.0	1.2997748789909589E-4
4	0.0	0.0	0.0	0.0	1.2997748789909589E-4
5	0.0	0.0	0.0	0.0	1.2997748789909589E-4
6	4.3325829299698624E-5	0.0	0.0	0.0	1.733033171987945E-4
7	4.3325829299698624E-5	0.0	0.0	0.0	1.733033171987945E-4
8	4.3325829299698624E-5	0.0	0.0	0.0	1.733033171987945E-4
9	4.3325829299698624E-5	0.0	0.0	0.0	1.733033171987945E-4
10	1.2997748789909589E-4	0.0	0.0	0.0	1.733033171987945E-4
11	1.2997748789909589E-4	0.0	0.0	0.0	1.733033171987945E-4
12	1.2997748789909589E-4	0.0	0.0	0.0	5.199099515963835E-4
13	1.2997748789909589E-4	0.0	0.0	0.0	5.632357808960821E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	40	0.0052871676	14.247139	1
GGTATCA	700	0.0	14.111452	1
TACGGTG	50	0.0015009767	13.298771	5
GTATCAA	2025	0.0	12.711027	1
CGACTAA	60	4.0913126E-4	12.667418	13
CGGTCCA	385	0.0	12.338126	10
CGAAATT	125	1.4097168E-9	12.160722	13
ACCGTTA	110	3.8149665E-8	12.091365	8
TCGCGTA	165	0.0	12.091364	9
CGAACGA	135	3.7107384E-10	11.964711	16
CGTCGTA	360	0.0	11.875447	10
AAGACGG	505	0.0	11.8503895	5
CAAGACG	525	0.0	11.759798	4
GTCCTAA	210	0.0	11.759543	1
TACCGTC	380	0.0	11.7504425	7
GCGAAAT	130	2.6138878E-9	11.693254	12
GCCGGTC	390	0.0	11.692749	8
CGCCGGT	370	0.0	11.554489	7
ACCGTCG	380	0.0	11.500433	8
CGCAAGA	485	0.0	11.356574	2
>>END_MODULE