FastQCFastQC Report
Thu 2 Feb 2017
SRR4064204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2308092
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26360.11420688603400557No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24610.10662486590655833No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT24370.10558504600336556No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23840.10328877705048152No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23410.10142576639059447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22600.028.7171
GGTATCA8050.024.596431
CGTCTTA1851.8189894E-1219.02673715
ACGTCGT1404.087269E-918.8568558
TCAACGC33850.018.782584
ATACCGT7700.018.5711446
ACCGTCG6800.018.4408958
ATCAACG34650.018.4124163
CAACGCA34800.018.2698365
AACGCAG35300.018.198026
TACCGTC7650.018.117377
ACCGTTA2800.018.0711548
CCGTTAT2850.017.7545019
CGTCGTA7100.017.66170310
GGACCGT4000.017.5997316
CGCAATA6100.017.31233636
AACGTCG1154.276908E-617.2171297
GGCGTTA6700.017.07436642
GTAATAC2201.8189894E-1216.999743
TTATACC2850.016.9821974
Produced by FastQC (version 0.11.5)