FastQCFastQC Report
Thu 2 Feb 2017
SRR4064203_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064203_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026314
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43930.4280366437561993No Hit
TATCAACGCAGAGTACTTTTTTTTT29680.28919024781889363No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28820.2808107460289931No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20930.20393368891002167No Hit
GTACATGGGGTGGTATCAACGCAAA18560.18084134095413296No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17800.17343619983747663No Hit
GGTATCAACGCAGAGTACTTTTTTT14840.14459512390944682No Hit
GTATCAACGCAGAGTACATGGGGTG12690.12364636943469542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAT653.381465E-614.6103611
TCCAACG2050.013.4416118
CGCGCTA500.001497318513.30191116
GTATTAA853.954796E-612.2898911
CCAACGA1405.2750693E-1112.21604219
TAAGACC701.0912215E-412.2130644
CGGTCCA1251.4078978E-912.15996910
ACTTACC550.003067281612.09087858
AAACTAT550.00306840212.0902886
ACGCACC550.003069522612.08974
ATGGTCG1201.0002623E-811.8743915
TTAATAC802.8699325E-511.8726543
TCTAGTA658.035926E-411.6894282
TAGGACA1001.929604E-611.3988584
GTATAAT752.0791325E-411.3960811
GTCTTAG1001.935272E-611.3960811
ATACGCT1604.5474735E-1111.281228
ACCGTCG1354.7184585E-911.2592318
GAACCGC1104.964568E-711.2266966
TGTACTG1451.2150849E-911.1373615
Produced by FastQC (version 0.11.5)