##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064203_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1026314
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125336885202774	32.0	32.0	32.0	32.0	32.0
2	30.74844833062786	32.0	32.0	32.0	32.0	32.0
3	30.771975243444015	32.0	32.0	32.0	32.0	32.0
4	30.783885828313753	32.0	32.0	32.0	32.0	32.0
5	30.73745072170895	32.0	32.0	32.0	32.0	32.0
6	34.361824938566556	36.0	36.0	36.0	32.0	36.0
7	34.26227938038456	36.0	36.0	36.0	32.0	36.0
8	34.220461769010264	36.0	36.0	36.0	32.0	36.0
9	34.33280945207802	36.0	36.0	36.0	32.0	36.0
10	34.059290821327586	36.0	36.0	36.0	32.0	36.0
11	34.35465851581485	36.0	36.0	36.0	32.0	36.0
12	34.15799258316655	36.0	36.0	36.0	32.0	36.0
13	34.242055550250704	36.0	36.0	36.0	32.0	36.0
14	34.138309523206345	36.0	36.0	36.0	32.0	36.0
15	34.09082600451713	36.0	36.0	36.0	32.0	36.0
16	34.08276024686402	36.0	36.0	36.0	32.0	36.0
17	34.01459787160655	36.0	36.0	36.0	32.0	36.0
18	34.00844478395501	36.0	36.0	36.0	32.0	36.0
19	34.012262329072776	36.0	36.0	36.0	32.0	36.0
20	33.97705965230914	36.0	36.0	36.0	32.0	36.0
21	33.96400321928766	36.0	36.0	36.0	32.0	36.0
22	33.94593077752033	36.0	36.0	36.0	32.0	36.0
23	33.90765301847193	36.0	36.0	36.0	32.0	36.0
24	33.904918962422805	36.0	36.0	36.0	32.0	36.0
25	33.52848738300364	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	10.0
5	14.0
6	60.0
7	19.0
8	68.0
9	71.0
10	105.0
11	29.0
12	67.0
13	46.0
14	149.0
15	259.0
16	369.0
17	529.0
18	649.0
19	949.0
20	1452.0
21	2175.0
22	3279.0
23	5099.0
24	7100.0
25	9880.0
26	13323.0
27	16873.0
28	22310.0
29	29035.0
30	37450.0
31	49440.0
32	67925.0
33	94387.0
34	201651.0
35	461542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.47683172061405	17.52088639613532	11.5260934958042	25.476188387446424
2	16.042940720212968	20.64921676157513	38.281922958493624	25.025919559718275
3	18.93241014018414	25.13707099239691	28.743253632247324	27.18726523517163
4	12.282293284587038	16.085303420360802	36.60727335797932	35.02512993707285
5	13.981999575130724	37.23024211701835	33.786754602912886	15.001003704938034
6	32.5529088798822	36.389576192977444	17.615243760421226	13.442271166719127
7	28.837459077986466	31.141177170024083	21.51745015043317	18.50391360155628
8	27.289147700909655	33.13327822638817	20.279875697447274	19.297698375254903
9	27.576051224657718	14.243738276939155	18.890164912513306	39.29004558588982
10	15.560945933297976	27.499083549121767	32.185169874894704	24.754800642685552
11	36.26508937119414	21.903400726512643	22.916735723714613	18.9147741785786
12	24.312503107812578	24.23762310594058	29.76792074419802	21.681953042048825
13	29.31508371935661	20.195996798062456	25.716121521524837	24.772797961056092
14	23.40834834055988	20.20978591423504	26.413953746866518	29.967911998338558
15	24.686909222442893	27.387021637753605	23.480857710296092	24.44521142950741
16	24.594203011594757	26.337121551895603	24.891871970172655	24.176803466336985
17	22.892716290724373	26.087778909856723	26.237928328027728	24.781576471391173
18	23.53417273202431	26.042422398527155	27.18245609929244	23.240948770156102
19	24.80846171623373	25.213234742026675	26.519605309732786	23.45869823200681
20	25.074065622434034	25.006387489492337	26.041648381868505	23.877898506205128
21	26.51656907723135	24.54661134702673	25.38693682932656	23.549882746415353
22	24.984226944766515	24.834053806840288	26.11774159103564	24.063977657357558
23	24.18755558676216	24.757466179843654	26.202624475338983	24.852353758055205
24	24.015594465875893	25.705515011331148	26.221657506835744	24.05723301595722
25	24.28219948335331	25.308569138307824	26.347128456622837	24.06210292171603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	238.0
1	238.0
2	548.0
3	858.0
4	858.0
5	858.0
6	2282.5
7	3707.0
8	3707.0
9	3707.0
10	3758.0
11	3809.0
12	3809.0
13	3809.0
14	3737.0
15	3665.0
16	3665.0
17	3665.0
18	6228.5
19	8792.0
20	8792.0
21	8792.0
22	13898.5
23	19005.0
24	19005.0
25	19005.0
26	27815.0
27	36625.0
28	36625.0
29	36625.0
30	46057.5
31	55490.0
32	55490.0
33	55490.0
34	63633.0
35	71776.0
36	71776.0
37	71776.0
38	79677.5
39	87579.0
40	87579.0
41	87579.0
42	98831.5
43	110084.0
44	110084.0
45	110084.0
46	120761.5
47	131439.0
48	131439.0
49	131439.0
50	133233.0
51	135027.0
52	135027.0
53	135027.0
54	123489.5
55	111952.0
56	111952.0
57	111952.0
58	100992.5
59	90033.0
60	90033.0
61	90033.0
62	78735.0
63	67437.0
64	67437.0
65	67437.0
66	55033.0
67	42629.0
68	42629.0
69	42629.0
70	32405.0
71	22181.0
72	22181.0
73	22181.0
74	16797.0
75	11413.0
76	11413.0
77	11413.0
78	9212.0
79	7011.0
80	7011.0
81	7011.0
82	4847.5
83	2684.0
84	2684.0
85	2684.0
86	2061.5
87	1439.0
88	1439.0
89	1439.0
90	1025.0
91	611.0
92	611.0
93	611.0
94	429.5
95	248.0
96	248.0
97	248.0
98	415.0
99	582.0
100	582.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03965647940104101
2	0.0060410361741143545
3	0.002046157413812927
4	0.004676931231572403
5	0.01130258380963331
6	0.015882078973881286
7	0.02698979064886575
8	0.04189750894950278
9	0.0543693255670292
10	0.0602154896064947
11	0.05768215185605964
12	0.06557447330933808
13	0.06674370611723118
14	0.06791293892512427
15	0.06362575196284957
16	0.0660616536459602
17	0.06518472904004038
18	0.07970270307137971
19	0.07804628992686449
20	0.08496425070689867
21	0.07395397509923864
22	0.08096937194659724
23	0.08671809991873831
24	0.08009244734067741
25	0.08330783756238344
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1026314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.58592426784427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.58175078629165	43.33449150292022
2	14.436643856259321	16.338216718642585
3	4.452507745797825	7.55847798317117
4	1.769578470001433	4.005329331580352
5	0.8299128023695763	2.348069149189963
6	0.47664971865334343	1.6182998929204422
7	0.27015973593618803	1.0701066850534158
8	0.19505175694205137	0.8829747157306309
9	0.13632374763931213	0.6942604733844147
>10	0.6986202554492013	7.210464039241593
>50	0.07442336810068167	2.9716336825633407
>100	0.070609036864011	7.774280822701021
>500	0.00638761397176947	2.3641697913899247
>1k	0.0013811057236258314	1.8292252115109435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4393	0.4280366437561993	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2968	0.28919024781889363	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2882	0.2808107460289931	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2093	0.20393368891002167	No Hit
GTACATGGGGTGGTATCAACGCAAA	1856	0.18084134095413296	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1780	0.17343619983747663	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1484	0.14459512390944682	No Hit
GTATCAACGCAGAGTACATGGGGTG	1269	0.12364636943469542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	9.743606732442508E-5	0.0	0.0	0.0
9	0.0	9.743606732442508E-5	0.0	9.743606732442508E-5	0.0
10	0.0	9.743606732442508E-5	0.0	1.9487213464885016E-4	0.0
11	0.0	9.743606732442508E-5	0.0	2.9230820197327526E-4	0.0
12	0.0	1.9487213464885016E-4	0.0	3.897442692977003E-4	9.743606732442508E-5
13	0.0	1.9487213464885016E-4	0.0	3.897442692977003E-4	9.743606732442508E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTAT	65	3.381465E-6	14.610361	1
TCCAACG	205	0.0	13.44161	18
CGCGCTA	50	0.0014973185	13.301911	16
GTATTAA	85	3.954796E-6	12.289891	1
CCAACGA	140	5.2750693E-11	12.216042	19
TAAGACC	70	1.0912215E-4	12.213064	4
CGGTCCA	125	1.4078978E-9	12.159969	10
ACTTACC	55	0.0030672816	12.0908785	8
AAACTAT	55	0.003068402	12.090288	6
ACGCACC	55	0.0030695226	12.0897	4
ATGGTCG	120	1.0002623E-8	11.874391	5
TTAATAC	80	2.8699325E-5	11.872654	3
TCTAGTA	65	8.035926E-4	11.689428	2
TAGGACA	100	1.929604E-6	11.398858	4
GTATAAT	75	2.0791325E-4	11.396081	1
GTCTTAG	100	1.935272E-6	11.396081	1
ATACGCT	160	4.5474735E-11	11.28122	8
ACCGTCG	135	4.7184585E-9	11.259231	8
GAACCGC	110	4.964568E-7	11.226696	6
TGTACTG	145	1.2150849E-9	11.137361	5
>>END_MODULE