FastQCFastQC Report
Thu 2 Feb 2017
SRR4064203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026314
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13340.12997971381078305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.1275438121276724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTGC450.001398728524.44246941
GCACCGT654.9341546E-420.3060516
TATACCG550.00448525519.9983845
TAGGACG1001.1239681E-619.79844
ATAGTAC808.992466E-519.2484443
ATTACAC1759.094947E-1218.8556183
CGCAATA2350.018.72462836
ATACCGT2000.018.6984886
AATCACG951.5958287E-518.52752534
CGTATGT2050.018.24598533
GTATCAA31600.018.0310651
ACGTATG1751.9099389E-1017.60200732
GATATAC4750.017.5994341
ATACGAA2800.017.28431740
AACGCAG33150.017.2535086
TATGGCG1802.8558134E-1017.11056116
CTAGCGG2750.016.8002829
GTATAGT1053.7744874E-516.7613661
CGAATGC2900.016.68830743
GGCGTTA2400.016.49866742
Produced by FastQC (version 0.11.5)