##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1026314
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229511630943357	32.0	32.0	32.0	32.0	32.0
2	31.395960690393	32.0	32.0	32.0	32.0	32.0
3	31.46202331839963	32.0	32.0	32.0	32.0	32.0
4	31.54912823950565	32.0	32.0	32.0	32.0	32.0
5	31.50656524221632	32.0	32.0	32.0	32.0	32.0
6	35.05567302014783	36.0	36.0	36.0	36.0	36.0
7	35.08288983683356	36.0	36.0	36.0	36.0	36.0
8	35.031340311054905	36.0	36.0	36.0	36.0	36.0
9	35.11694081928143	36.0	36.0	36.0	36.0	36.0
10	34.99506583755069	36.0	36.0	36.0	36.0	36.0
11	35.121676212153396	36.0	36.0	36.0	36.0	36.0
12	35.04314761369328	36.0	36.0	36.0	36.0	36.0
13	35.07052227680807	36.0	36.0	36.0	36.0	36.0
14	35.02150998622254	36.0	36.0	36.0	36.0	36.0
15	35.00203543944641	36.0	36.0	36.0	36.0	36.0
16	35.003798057904305	36.0	36.0	36.0	36.0	36.0
17	34.96688732688047	36.0	36.0	36.0	36.0	36.0
18	34.963777167611475	36.0	36.0	36.0	36.0	36.0
19	34.96077029057384	36.0	36.0	36.0	36.0	36.0
20	34.95056678560363	36.0	36.0	36.0	36.0	36.0
21	34.94473328825291	36.0	36.0	36.0	36.0	36.0
22	34.93499455332384	36.0	36.0	36.0	36.0	36.0
23	34.88433851628254	36.0	36.0	36.0	32.0	36.0
24	34.8656580734551	36.0	36.0	36.0	32.0	36.0
25	34.83621776571303	36.0	36.0	36.0	32.0	36.0
26	34.7801842321161	36.0	36.0	36.0	32.0	36.0
27	34.78097736170412	36.0	36.0	36.0	32.0	36.0
28	34.751197976447756	36.0	36.0	36.0	32.0	36.0
29	34.71359350062456	36.0	36.0	36.0	32.0	36.0
30	34.70428640747374	36.0	36.0	36.0	32.0	36.0
31	34.68689114637431	36.0	36.0	36.0	32.0	36.0
32	34.63886978059347	36.0	36.0	36.0	32.0	36.0
33	34.59991484087716	36.0	36.0	36.0	32.0	36.0
34	34.573067306886585	36.0	36.0	36.0	32.0	36.0
35	34.52561886518161	36.0	36.0	36.0	32.0	36.0
36	34.49957517874647	36.0	36.0	36.0	32.0	36.0
37	34.48213802013809	36.0	36.0	36.0	32.0	36.0
38	34.4359279908488	36.0	36.0	36.0	32.0	36.0
39	34.43191654795706	36.0	36.0	36.0	32.0	36.0
40	34.39147570821405	36.0	36.0	36.0	32.0	36.0
41	34.361477091806215	36.0	36.0	36.0	32.0	36.0
42	34.29215522734758	36.0	36.0	36.0	32.0	36.0
43	34.27261637276701	36.0	36.0	36.0	32.0	36.0
44	34.19644572713614	36.0	36.0	36.0	32.0	36.0
45	34.144726662600334	36.0	36.0	36.0	32.0	36.0
46	34.100934022141374	36.0	36.0	36.0	32.0	36.0
47	34.04287771578679	36.0	36.0	36.0	32.0	36.0
48	33.97424277560279	36.0	36.0	36.0	32.0	36.0
49	33.93616183741038	36.0	36.0	36.0	32.0	36.0
50	33.35247205046409	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	4.0
20	18.0
21	50.0
22	203.0
23	515.0
24	1151.0
25	2520.0
26	5035.0
27	8752.0
28	14189.0
29	20872.0
30	28460.0
31	38988.0
32	54052.0
33	83204.0
34	167114.0
35	601183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71677086906358	18.340336462003805	12.088484985500015	25.8544076834326
2	15.427097634147934	21.380556186185277	37.75522234488376	25.437123834783026
3	18.208418305952094	25.630729866482966	29.072669005788693	27.088182821776247
4	11.986195257981477	16.628926429923006	36.73485892231812	34.6500193897774
5	13.689572586947074	37.621624571037714	33.518299467804205	15.17050337421101
6	32.48362586888613	36.79185902170291	17.385614928764493	13.338900180646467
7	28.64772116081659	31.308900217477724	21.512756354792696	18.530622266912985
8	27.371545160642846	33.32420682169395	20.100865816894245	19.203382200768964
9	27.618619824145675	14.089879801696185	18.914668558243953	39.376831815914194
10	15.43801836492891	27.665194718134202	32.26129879645797	24.63548812047892
11	36.70420232424221	21.711992345415094	22.918057161898954	18.66574816844374
12	24.328153302160256	24.336143067465773	29.757296263420614	21.578407366953357
13	29.440588671465086	20.212099451335902	25.78779726613083	24.559514611068185
14	23.48106618650079	19.963130120275316	26.529359493019665	30.026444200204228
15	24.51216921730759	27.469360153876437	23.527835537739286	24.490635091076687
16	24.606721757287524	26.281948224170304	24.989160184700708	24.122169833841465
17	22.979419553859735	25.922281095259347	26.330343345214036	24.767956005666882
18	23.69359774961001	25.813520463095497	27.40621507515753	23.086666712136964
19	24.94869991055369	24.936910146407435	26.757600500431643	23.356789442607234
20	25.066914806027444	24.883441082329327	26.37149775164303	23.678146360000195
21	26.44970252768646	24.382206615129483	25.738321800150832	23.42976905703323
22	24.897058794871747	24.77009959914802	26.395528074254077	23.937313531726158
23	24.16769624713049	24.473452413895245	26.510949929261002	24.847901409713263
24	24.02760956474207	25.415882710016753	26.639878165585284	23.916629559655895
25	24.381902261924946	24.848922433532692	26.69264362843396	24.076531676108406
26	23.830330006986593	25.75744140566002	27.071934643669042	23.340293943684344
27	24.16167679257877	25.223119235660103	26.66234671058081	23.95285726118032
28	23.651688404067176	25.52683761016891	26.881945696287023	23.93952828947689
29	23.582544166237128	25.186764403537783	26.767209753939664	24.46348167628543
30	23.59696064980824	25.831943188550404	26.749341305229024	23.821754856412337
31	24.101933966263022	25.55072775870587	25.994584075935972	24.352754199095138
32	23.895019414685002	25.7842607073084	26.01899082613504	24.301729051871558
33	24.057458675511658	25.104943348131286	26.402557616529016	24.435040359828037
34	24.299830359343925	25.391045584714604	26.608248959599017	23.700875096342447
35	24.721395880449432	25.44478732350038	26.35526276044382	23.478554035606372
36	23.842250864079155	26.09650597279186	25.992144114858455	24.069099048270527
37	24.349250398040297	25.88362997160613	26.020045250896928	23.747074379456638
38	23.912736315596124	25.862312577285834	26.238936371529388	23.98601473558866
39	24.222626272074958	25.57752495430079	25.99700275559401	24.20284601803024
40	24.80938605012818	25.95146201508542	25.91034299305556	23.328808941730838
41	23.607306870434304	25.971311559272586	26.78318605197283	23.63819551832028
42	24.671050067426943	26.403103977799773	25.85267797984207	23.07316797493121
43	24.03395881524152	25.603477339028863	26.17786967188995	24.18469417383967
44	23.72522157350316	26.224890285063957	25.573811064254365	24.476077077178513
45	23.789799638895953	26.29862505687925	25.8312164997072	24.0803588045176
46	23.684302835428237	26.027282471012374	25.579265322030597	24.709149371528795
47	23.87050129787118	26.056615245504222	25.904125125694733	24.16875833092987
48	24.7026451355794	26.04767226704888	25.036465981161438	24.213216616210286
49	23.983837273655574	26.435723249849946	25.315985782101347	24.26445369439313
50	24.11003378144661	26.51750993614946	25.11363603146439	24.258820250939536
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	36.0
2	32.0
3	100.5
4	169.0
5	211.5
6	254.0
7	273.5
8	293.0
9	405.5
10	518.0
11	831.5
12	1145.0
13	2025.0
14	2905.0
15	4079.5
16	5254.0
17	5721.0
18	6188.0
19	6268.0
20	6348.0
21	6756.0
22	7164.0
23	7832.5
24	8501.0
25	8873.0
26	9245.0
27	11445.5
28	13646.0
29	15388.0
30	17130.0
31	19224.0
32	21318.0
33	24353.5
34	27389.0
35	30792.5
36	34196.0
37	38365.5
38	42535.0
39	45434.5
40	48334.0
41	51798.5
42	55263.0
43	56016.0
44	56769.0
45	61197.5
46	65626.0
47	68779.0
48	71932.0
49	73889.0
50	75846.0
51	73318.5
52	70791.0
53	69132.5
54	67474.0
55	67619.5
56	67765.0
57	65239.5
58	62714.0
59	56563.0
60	50412.0
61	44233.0
62	38054.0
63	32928.5
64	27803.0
65	23615.5
66	19428.0
67	16738.0
68	14048.0
69	12607.0
70	11166.0
71	8808.0
72	6450.0
73	5554.0
74	4658.0
75	3663.5
76	2669.0
77	2336.0
78	2003.0
79	1612.0
80	1221.0
81	996.5
82	772.0
83	600.0
84	428.0
85	311.0
86	194.0
87	140.0
88	86.0
89	63.0
90	40.0
91	35.5
92	31.0
93	28.0
94	25.0
95	24.0
96	23.0
97	19.0
98	15.0
99	9.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010328223136389059
2	2.923082019732752E-4
3	2.923082019732752E-4
4	0.0
5	0.0
6	0.0
7	1.9487213464885013E-4
8	0.0
9	9.743606732442508E-4
10	9.743606732442508E-4
11	9.743606732442507E-5
12	9.743606732442507E-5
13	2.923082019732752E-4
14	1.9487213464885013E-4
15	0.0035076984236793026
16	4.871803366221254E-4
17	0.0
18	2.923082019732752E-4
19	0.0
20	8.769246059198257E-4
21	0.0
22	0.0
23	5.846164039465504E-4
24	2.923082019732752E-4
25	0.0019487213464885016
26	0.006138472241438779
27	0.006723088645385331
28	0.004774367298896829
29	0.002046157413812927
30	0.004092314827625854
31	0.007892321453278431
32	0.0028256459524083274
33	0.004482059096923553
34	0.002630773817759477
35	0.0036051344910037275
36	0.0075025771839807315
37	0.0035076984236793026
38	0.008866682126522682
39	0.0037025705583281533
40	0.002435901683110627
41	0.004579495164247978
42	0.0017538492118396513
43	0.0011692328078931008
44	0.0017538492118396513
45	0.0016564131445152264
46	0.001364104942541951
47	0.0017538492118396513
48	0.0014615410098663762
49	1.9487213464885013E-4
50	0.001071796740568676
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1026314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.06914225537603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.44045056925732	46.51781441565133
2	14.081008667749058	16.916682255244023
3	4.282410101549835	7.717221047575691
4	1.6816909231249135	4.040709251630603
5	0.7752895909825737	2.3285490364922254
6	0.43975524728743204	1.5849432304114088
7	0.26293813234418495	1.1056127653302015
8	0.1749771793202266	0.8408583260824906
9	0.12801841147418688	0.6920960553135175
>10	0.6061617327680097	6.652521178267494
>50	0.06284156162415275	2.6925430211555046
>100	0.060204317392160575	7.034136182636084
>500	0.003926367808504869	1.6165795161968046
>1k	3.2719731737540573E-4	0.2597337180126538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1334	0.12997971381078305	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1309	0.1275438121276724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9487213464885016E-4
2	0.0	0.0	0.0	0.0	1.9487213464885016E-4
3	0.0	0.0	0.0	0.0	1.9487213464885016E-4
4	0.0	0.0	0.0	0.0	1.9487213464885016E-4
5	0.0	0.0	0.0	0.0	1.9487213464885016E-4
6	0.0	0.0	0.0	0.0	1.9487213464885016E-4
7	0.0	0.0	0.0	0.0	1.9487213464885016E-4
8	0.0	0.0	0.0	0.0	1.9487213464885016E-4
9	0.0	0.0	0.0	0.0	1.9487213464885016E-4
10	0.0	0.0	0.0	9.743606732442508E-5	1.9487213464885016E-4
11	0.0	0.0	0.0	9.743606732442508E-5	2.9230820197327526E-4
12	0.0	0.0	0.0	1.9487213464885016E-4	6.820524712709755E-4
13	0.0	0.0	0.0	1.9487213464885016E-4	6.820524712709755E-4
14	0.0	0.0	0.0	1.9487213464885016E-4	6.820524712709755E-4
15	0.0	0.0	0.0	1.9487213464885016E-4	6.820524712709755E-4
16	0.0	0.0	0.0	1.9487213464885016E-4	7.794885385954006E-4
17	0.0	0.0	0.0	1.9487213464885016E-4	7.794885385954006E-4
18	0.0	0.0	0.0	1.9487213464885016E-4	8.769246059198257E-4
19	0.0	0.0	0.0	1.9487213464885016E-4	8.769246059198257E-4
20	0.0	9.743606732442508E-5	0.0	2.9230820197327526E-4	8.769246059198257E-4
21	0.0	9.743606732442508E-5	0.0	3.897442692977003E-4	8.769246059198257E-4
22	0.0	9.743606732442508E-5	0.0	6.820524712709755E-4	8.769246059198257E-4
23	0.0	9.743606732442508E-5	0.0	0.001364104942541951	8.769246059198257E-4
24	0.0	9.743606732442508E-5	0.0	0.002338465615786202	8.769246059198257E-4
25	0.0	9.743606732442508E-5	0.0	0.002630773817759477	8.769246059198257E-4
26	0.0	9.743606732442508E-5	0.0	0.0030205180870571773	8.769246059198257E-4
27	0.0	9.743606732442508E-5	0.0	0.0038000066256525782	8.769246059198257E-4
28	0.0	9.743606732442508E-5	0.0	0.0060410361741143545	8.769246059198257E-4
29	0.0	9.743606732442508E-5	0.0	0.013738485492743936	8.769246059198257E-4
30	0.0	9.743606732442508E-5	0.0	0.027282098850839022	8.769246059198257E-4
31	0.0	9.743606732442508E-5	0.0	0.05563599444224672	8.769246059198257E-4
32	0.0	9.743606732442508E-5	0.0	0.0823334768891392	8.769246059198257E-4
33	0.0	9.743606732442508E-5	0.0	0.10864121506673396	8.769246059198257E-4
34	0.0	9.743606732442508E-5	0.0	0.14108742548576753	8.769246059198257E-4
35	0.0	9.743606732442508E-5	0.0	0.17372850803944992	8.769246059198257E-4
36	0.0	9.743606732442508E-5	0.0	0.22273884990363574	8.769246059198257E-4
37	0.0	9.743606732442508E-5	0.0	0.29474410365638587	8.769246059198257E-4
38	0.0	9.743606732442508E-5	0.0	0.3982212071549253	8.769246059198257E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTGC	45	0.0013987285	24.442469	41
GCACCGT	65	4.9341546E-4	20.306051	6
TATACCG	55	0.004485255	19.998384	5
TAGGACG	100	1.1239681E-6	19.7984	4
ATAGTAC	80	8.992466E-5	19.248444	3
ATTACAC	175	9.094947E-12	18.855618	3
CGCAATA	235	0.0	18.724628	36
ATACCGT	200	0.0	18.698488	6
AATCACG	95	1.5958287E-5	18.527525	34
CGTATGT	205	0.0	18.245985	33
GTATCAA	3160	0.0	18.031065	1
ACGTATG	175	1.9099389E-10	17.602007	32
GATATAC	475	0.0	17.599434	1
ATACGAA	280	0.0	17.284317	40
AACGCAG	3315	0.0	17.253508	6
TATGGCG	180	2.8558134E-10	17.110561	16
CTAGCGG	275	0.0	16.80028	29
GTATAGT	105	3.7744874E-5	16.761366	1
CGAATGC	290	0.0	16.688307	43
GGCGTTA	240	0.0	16.498667	42
>>END_MODULE