FastQCFastQC Report
Thu 2 Feb 2017
SRR4064202_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064202_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538641
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38530.2504157889982134No Hit
TATCAACGCAGAGTACTTTTTTTTT25960.16872031877481491No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21730.14122852569247799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1004.0017767E-1115.1978671
TACGCAC602.5661113E-514.2512459
TAACGAA954.8949005E-914.00122213
CGTTATT1153.783498E-1013.21338752
AATCCCG604.0878466E-412.66818419
AACGAAC1051.9908839E-812.66777214
AACCGCG1207.403287E-1012.6669487
TAACCAG2150.012.3699535
AATAACG2250.012.2407352
ACGCACG701.0896659E-412.2157510
AACGCCG2100.012.2121745
CGAGCCG1800.012.14034415
CGATAAC1103.806963E-812.09235910
ACGCCGC2200.012.0903926
CGCATCG2150.011.93127313
TAACGCC2250.011.8197944
ATAACGC2500.011.7764323
AGAACCG1950.011.6902875
GTATTAG2200.011.6574551
CCGTCGT1800.011.6121249
Produced by FastQC (version 0.11.5)