##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064202_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1538641
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200530208151218	32.0	32.0	32.0	32.0	32.0
2	30.806252400657463	32.0	32.0	32.0	32.0	32.0
3	30.8256441886054	32.0	32.0	32.0	32.0	32.0
4	30.853820351855955	32.0	32.0	32.0	32.0	32.0
5	30.76558469454538	32.0	32.0	32.0	32.0	32.0
6	34.4058737548265	36.0	36.0	36.0	32.0	36.0
7	34.34477048252322	36.0	36.0	36.0	32.0	36.0
8	34.29849523053136	36.0	36.0	36.0	32.0	36.0
9	34.43030570483953	36.0	36.0	36.0	32.0	36.0
10	34.13552089148801	36.0	36.0	36.0	32.0	36.0
11	34.41922839700749	36.0	36.0	36.0	32.0	36.0
12	34.234432203483465	36.0	36.0	36.0	32.0	36.0
13	34.315475149823776	36.0	36.0	36.0	32.0	36.0
14	34.21984010565168	36.0	36.0	36.0	32.0	36.0
15	34.16143856819102	36.0	36.0	36.0	32.0	36.0
16	34.15417436556026	36.0	36.0	36.0	32.0	36.0
17	34.089871516487605	36.0	36.0	36.0	32.0	36.0
18	34.0720200488613	36.0	36.0	36.0	32.0	36.0
19	34.097343694858	36.0	36.0	36.0	32.0	36.0
20	34.07087683221752	36.0	36.0	36.0	32.0	36.0
21	34.061727199522174	36.0	36.0	36.0	32.0	36.0
22	34.052388438888606	36.0	36.0	36.0	32.0	36.0
23	33.987611795084106	36.0	36.0	36.0	32.0	36.0
24	33.98349452536362	36.0	36.0	36.0	32.0	36.0
25	33.582968346742355	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	11.0
5	19.0
6	57.0
7	20.0
8	102.0
9	101.0
10	147.0
11	34.0
12	107.0
13	85.0
14	146.0
15	277.0
16	472.0
17	644.0
18	860.0
19	1061.0
20	1707.0
21	2466.0
22	3942.0
23	5938.0
24	8973.0
25	12741.0
26	17857.0
27	23764.0
28	32174.0
29	42967.0
30	56085.0
31	75536.0
32	105696.0
33	147892.0
34	312903.0
35	683857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.75698922074553	16.86632463117187	11.169294566420922	26.207391581661675
2	16.678690834594246	19.7697469335122	37.32334222471805	26.2282200071755
3	18.80123513518959	23.966198215670307	28.48018707709586	28.752379572044244
4	12.739985740107281	15.432531663916297	35.78472631600157	36.04275627997486
5	14.562158964975966	36.65196634416954	33.777311238434464	15.008563452420029
6	34.03618177729071	35.40112346550498	17.00752833688885	13.555166420315459
7	30.009933081712596	30.376377987562847	21.149598191243026	18.464090739481527
8	28.138045730476385	32.81043677942707	19.603549576457695	19.44796791363885
9	27.610169590715522	14.026721217989191	18.351703872671386	40.0114053186239
10	15.664989328592219	26.959448650434336	32.19338073580039	25.18218128517305
11	36.99904864029694	20.988633755126646	22.62649409968403	19.385823504892382
12	24.67762704368976	23.594032490927773	29.143438731579153	22.58490173380331
13	29.7858475601944	19.577366137653538	25.030777625235505	25.606008676916552
14	23.476036651472178	19.40649796861251	25.57759757130993	31.539867808605383
15	25.078476524675153	26.958856645646385	22.48158150925948	25.481085320418984
16	25.53703335167344	25.567405083460475	23.931038609169793	24.964522955696296
17	23.99680037459029	25.699170178450654	25.350463035221892	24.953566411737164
18	24.538621542832388	25.083221785570725	26.1119978562016	24.266158815395283
19	25.52188482111266	24.881691364705393	25.441559826836226	24.154863987345724
20	25.834512795429237	24.35860244148654	25.095874657869267	24.711010105214957
21	26.338913467379694	24.299144443042437	24.60991311745599	24.752028972121877
22	25.68723141175866	24.365421152216317	25.010195539719675	24.937151896305345
23	24.684820519091595	24.275487225653027	25.35583311326167	25.683859141993704
24	24.721798545007527	24.882842861463512	25.380220037529313	25.01513855599965
25	24.876255494516215	24.541413594789265	25.34502114551313	25.237309765181386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	272.0
1	272.0
2	454.0
3	636.0
4	636.0
5	636.0
6	1861.5
7	3087.0
8	3087.0
9	3087.0
10	3195.5
11	3304.0
12	3304.0
13	3304.0
14	3832.5
15	4361.0
16	4361.0
17	4361.0
18	7327.5
19	10294.0
20	10294.0
21	10294.0
22	15972.5
23	21651.0
24	21651.0
25	21651.0
26	31514.5
27	41378.0
28	41378.0
29	41378.0
30	52997.5
31	64617.0
32	64617.0
33	64617.0
34	79249.5
35	93882.0
36	93882.0
37	93882.0
38	109386.0
39	124890.0
40	124890.0
41	124890.0
42	142962.5
43	161035.0
44	161035.0
45	161035.0
46	180426.5
47	199818.0
48	199818.0
49	199818.0
50	206132.5
51	212447.0
52	212447.0
53	212447.0
54	199997.0
55	187547.0
56	187547.0
57	187547.0
58	171441.5
59	155336.0
60	155336.0
61	155336.0
62	134284.0
63	113232.0
64	113232.0
65	113232.0
66	92083.0
67	70934.0
68	70934.0
69	70934.0
70	53237.5
71	35541.0
72	35541.0
73	35541.0
74	26521.0
75	17501.0
76	17501.0
77	17501.0
78	13581.0
79	9661.0
80	9661.0
81	9661.0
82	6611.5
83	3562.0
84	3562.0
85	3562.0
86	2680.0
87	1798.0
88	1798.0
89	1798.0
90	1264.0
91	730.0
92	730.0
93	730.0
94	518.0
95	306.0
96	306.0
97	306.0
98	563.5
99	821.0
100	821.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038605496668813584
2	0.00474444656030874
3	0.0015598180472247913
4	0.0038995451180619785
5	0.008189044747930155
6	0.012153582284626499
7	0.022552369266125107
8	0.035225890899826535
9	0.04952422299938712
10	0.057648275328682906
11	0.05472361649013643
12	0.06375756268031334
13	0.06486243379709758
14	0.06642225184432236
15	0.05979302514361699
16	0.0667472139374942
17	0.06245771430762601
18	0.07688603124445532
19	0.0738963799872745
20	0.07909577347802378
21	0.075586182871768
22	0.07825087203577703
23	0.08208542473520464
24	0.07643108431401477
25	0.07792590994260519
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1538641.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.602927692723945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.62531840460545	43.37217357087832
2	13.837099824732718	15.664407217126982
3	4.2205479123930205	7.166861049265773
4	1.823986357228763	4.129718715629385
5	0.9760096134868412	2.762250078979956
6	0.57871318568668	1.965411636254944
7	0.3717217346450505	1.4728376927549398
8	0.2694029898555414	1.2199198363997483
9	0.19290503002355194	0.9827090519387266
>10	0.9837006911490457	9.717043943284578
>50	0.06033125780923413	2.376511570133416
>100	0.054721689076712696	6.348704249254097
>500	0.004386869868354105	1.7150357388497086
>1k	0.0011544394390405539	1.1064156492495338
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3853	0.2504157889982134	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2596	0.16872031877481491	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2173	0.14122852569247799	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.299848372687326E-4
2	0.0	0.0	0.0	0.0	1.299848372687326E-4
3	0.0	0.0	0.0	0.0	1.299848372687326E-4
4	0.0	0.0	0.0	6.49924186343663E-5	1.299848372687326E-4
5	0.0	6.49924186343663E-5	0.0	6.49924186343663E-5	1.299848372687326E-4
6	0.0	6.49924186343663E-5	0.0	6.49924186343663E-5	2.599696745374652E-4
7	0.0	6.49924186343663E-5	0.0	6.49924186343663E-5	2.599696745374652E-4
8	0.0	6.49924186343663E-5	0.0	6.49924186343663E-5	2.599696745374652E-4
9	0.0	6.49924186343663E-5	0.0	6.49924186343663E-5	2.599696745374652E-4
10	6.49924186343663E-5	6.49924186343663E-5	0.0	6.49924186343663E-5	2.599696745374652E-4
11	6.49924186343663E-5	6.49924186343663E-5	0.0	1.299848372687326E-4	2.599696745374652E-4
12	6.49924186343663E-5	6.49924186343663E-5	0.0	1.299848372687326E-4	2.599696745374652E-4
13	6.49924186343663E-5	6.49924186343663E-5	0.0	1.299848372687326E-4	5.849317677092968E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	100	4.0017767E-11	15.197867	1
TACGCAC	60	2.5661113E-5	14.251245	9
TAACGAA	95	4.8949005E-9	14.001222	13
CGTTATT	115	3.783498E-10	13.2133875	2
AATCCCG	60	4.0878466E-4	12.668184	19
AACGAAC	105	1.9908839E-8	12.667772	14
AACCGCG	120	7.403287E-10	12.666948	7
TAACCAG	215	0.0	12.369953	5
AATAACG	225	0.0	12.240735	2
ACGCACG	70	1.0896659E-4	12.21575	10
AACGCCG	210	0.0	12.212174	5
CGAGCCG	180	0.0	12.140344	15
CGATAAC	110	3.806963E-8	12.092359	10
ACGCCGC	220	0.0	12.090392	6
CGCATCG	215	0.0	11.931273	13
TAACGCC	225	0.0	11.819794	4
ATAACGC	250	0.0	11.776432	3
AGAACCG	195	0.0	11.690287	5
GTATTAG	220	0.0	11.657455	1
CCGTCGT	180	0.0	11.612124	9
>>END_MODULE