##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1538641
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289490530929566	32.0	32.0	32.0	32.0	32.0
2	31.37835726462508	32.0	32.0	32.0	32.0	32.0
3	31.455816529001893	32.0	32.0	32.0	32.0	32.0
4	31.5465680428378	32.0	32.0	32.0	32.0	32.0
5	31.494748937536436	32.0	32.0	32.0	32.0	32.0
6	35.066691970381655	36.0	36.0	36.0	36.0	36.0
7	35.0891052558719	36.0	36.0	36.0	36.0	36.0
8	35.03517064734399	36.0	36.0	36.0	36.0	36.0
9	35.13520177871251	36.0	36.0	36.0	36.0	36.0
10	34.992737747141796	36.0	36.0	36.0	36.0	36.0
11	35.14450414359165	36.0	36.0	36.0	36.0	36.0
12	35.05177686022925	36.0	36.0	36.0	36.0	36.0
13	35.09284686941268	36.0	36.0	36.0	36.0	36.0
14	35.038223341247246	36.0	36.0	36.0	36.0	36.0
15	35.014104654692034	36.0	36.0	36.0	36.0	36.0
16	35.02402119792726	36.0	36.0	36.0	36.0	36.0
17	34.987992650657304	36.0	36.0	36.0	36.0	36.0
18	34.98182811974983	36.0	36.0	36.0	36.0	36.0
19	34.9800070321797	36.0	36.0	36.0	36.0	36.0
20	34.97047069459347	36.0	36.0	36.0	36.0	36.0
21	34.96884523420343	36.0	36.0	36.0	36.0	36.0
22	34.95490111078543	36.0	36.0	36.0	36.0	36.0
23	34.90785114916345	36.0	36.0	36.0	32.0	36.0
24	34.87360534393663	36.0	36.0	36.0	32.0	36.0
25	34.852168894498455	36.0	36.0	36.0	32.0	36.0
26	34.78811496638917	36.0	36.0	36.0	32.0	36.0
27	34.78327108142835	36.0	36.0	36.0	32.0	36.0
28	34.75806637155776	36.0	36.0	36.0	32.0	36.0
29	34.738874760259215	36.0	36.0	36.0	32.0	36.0
30	34.71739086635544	36.0	36.0	36.0	32.0	36.0
31	34.709080285784665	36.0	36.0	36.0	32.0	36.0
32	34.6707295593969	36.0	36.0	36.0	32.0	36.0
33	34.6464100462681	36.0	36.0	36.0	32.0	36.0
34	34.631633369967396	36.0	36.0	36.0	32.0	36.0
35	34.597033356059015	36.0	36.0	36.0	32.0	36.0
36	34.57103768845364	36.0	36.0	36.0	32.0	36.0
37	34.55912197842122	36.0	36.0	36.0	32.0	36.0
38	34.5240052747847	36.0	36.0	36.0	32.0	36.0
39	34.51357269174551	36.0	36.0	36.0	32.0	36.0
40	34.50119683538915	36.0	36.0	36.0	32.0	36.0
41	34.48225414505398	36.0	36.0	36.0	32.0	36.0
42	34.42380711290028	36.0	36.0	36.0	32.0	36.0
43	34.41079043129619	36.0	36.0	36.0	32.0	36.0
44	34.35845918573598	36.0	36.0	36.0	32.0	36.0
45	34.31585015607929	36.0	36.0	36.0	32.0	36.0
46	34.305544958180626	36.0	36.0	36.0	32.0	36.0
47	34.269477415459484	36.0	36.0	36.0	32.0	36.0
48	34.21707207854204	36.0	36.0	36.0	32.0	36.0
49	34.199403239612096	36.0	36.0	36.0	32.0	36.0
50	33.60309909849016	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	0.0
19	5.0
20	17.0
21	47.0
22	136.0
23	514.0
24	1243.0
25	3017.0
26	5962.0
27	10990.0
28	18001.0
29	27496.0
30	40099.0
31	56003.0
32	80928.0
33	129670.0
34	265408.0
35	899103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.800503343490334	17.798106222115916	11.699941241927265	26.701449192466487
2	15.95155183103974	20.518719851504645	36.81525301013757	26.714475307318047
3	18.110013096023422	24.44692860879936	28.878388961644575	28.564669333532645
4	12.379755901474093	16.051177630129445	35.9323584903821	35.636707978014364
5	14.21130725100917	37.018966737530064	33.509181154018385	15.26054485744238
6	34.175852815377745	35.7377526502318	16.69358439504003	13.392810139350425
7	29.957689907970675	30.509605885717257	21.087778817657153	18.444925388654916
8	28.049576249155102	33.06302968855613	19.44255966307908	19.444834399209693
9	27.656457571290787	13.80008683060493	18.18158775220521	40.36186784589908
10	15.473805850154617	27.077860376725727	32.35321644116244	25.095117331957212
11	37.233831673535285	20.859381753118498	22.619506434574408	19.287280138771813
12	24.60060533938716	23.527905469826944	29.128302183550286	22.743187007235605
13	29.821471397012804	19.558140245269666	25.06556144317716	25.554826914540374
14	23.49179629996718	19.239130788003653	25.581310707217764	31.68776220481141
15	25.14480922619357	27.010560414305175	22.42677644783228	25.41785391166897
16	25.531952230075554	25.533837029815583	23.89198147696807	25.04222926314079
17	23.96309470500266	25.770533867224387	25.33664448042136	24.92972694735159
18	24.638559972235168	24.968722317219722	26.138003960648287	24.254713749896826
19	25.57674309780065	24.818541049238288	25.390344720012482	24.21437113294858
20	25.95060648384952	24.213143830554984	25.11407927737984	24.722170408215657
21	26.506248046165414	24.283507328870087	24.63784599526465	24.572398629699848
22	25.730142028117058	24.269370528109583	25.027508076943324	24.972979366830035
23	24.712942869342942	24.098240578800244	25.425069428602438	25.763747123254372
24	24.793193451862557	24.795273216017303	25.399574688230352	25.01195864388978
25	25.01429863876959	24.326794089202707	25.558361843952454	25.10054542807525
26	24.850363813033574	24.979510127943897	25.571555312905208	24.598570746117325
27	25.20719197113644	24.822413845772868	24.924588037170086	25.045806145920608
28	24.62106120411576	24.72797845796072	25.564597844886137	25.086362493037377
29	24.708778027860337	24.781051382325394	25.355793534784475	25.15437705502979
30	24.687521732602708	24.907073828129317	25.581718810830978	24.823685628437
31	25.122227060895792	24.662177533099776	24.964674072003795	25.250921334000637
32	24.907318100398026	24.8119064393772	24.811971433696694	25.46880402652808
33	24.555694211545713	24.661571059028457	25.29670215393415	25.486032575491684
34	25.21553643142513	24.6335149483005	25.359043235974454	24.791905384299913
35	25.525609061768	24.53984021796495	25.364488143736235	24.57006257653081
36	24.83602780068936	25.052144166840534	24.994231480452356	25.117596552017744
37	25.358656069091783	24.880881273840807	24.933721827308233	24.826740829759174
38	24.787199746251623	24.791749537871166	25.308540868828207	25.112509847049004
39	25.261573378985997	24.602976522416213	24.96649494921921	25.16895514937858
40	25.42224608203341	24.85192871260359	25.223366178847627	24.50245902651537
41	24.716510842684396	24.981557849856525	25.431838787985146	24.870092519473936
42	25.502937389632596	25.142222433090627	25.033358009269403	24.32148216800738
43	24.862994823960047	24.50761327624351	25.351611955586407	25.27777994421004
44	24.710081676928006	24.89901684818178	25.034462633146653	25.35643884174356
45	24.648322137182312	25.029116925252694	25.1496142007404	25.172946736824592
46	24.6363483334839	24.86427852328274	25.18319880880966	25.316174334423696
47	24.828319067210014	24.869135128460105	25.340989594504133	24.96155620982575
48	25.236268910774484	25.256481999626935	24.723792365431848	24.78345672416673
49	24.709531877889326	25.168769501838316	24.897295463452394	25.224403156819964
50	24.754000977496776	25.33659945509754	24.89328126949794	25.01611829790774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	121.0
1	90.0
2	59.0
3	171.0
4	283.0
5	309.5
6	336.0
7	304.0
8	272.0
9	400.5
10	529.0
11	807.5
12	1086.0
13	1810.0
14	2534.0
15	3336.0
16	4138.0
17	4408.5
18	4679.0
19	4701.5
20	4724.0
21	5260.5
22	5797.0
23	6737.5
24	7678.0
25	8634.5
26	9591.0
27	11685.5
28	13780.0
29	16008.5
30	18237.0
31	21092.0
32	23947.0
33	27914.0
34	31881.0
35	36303.5
36	40726.0
37	46972.5
38	53219.0
39	59042.5
40	64866.0
41	70619.0
42	76372.0
43	79655.5
44	82939.0
45	90953.0
46	98967.0
47	105580.5
48	112194.0
49	116156.0
50	120118.0
51	119589.0
52	119060.0
53	118003.0
54	116946.0
55	115138.5
56	113331.0
57	109937.0
58	106543.0
59	97187.5
60	87832.0
61	77556.5
62	67281.0
63	58101.5
64	48922.0
65	41547.0
66	34172.0
67	28463.5
68	22755.0
69	19794.0
70	16833.0
71	13130.5
72	9428.0
73	7942.5
74	6457.0
75	5083.0
76	3709.0
77	3145.0
78	2581.0
79	2086.0
80	1591.0
81	1266.0
82	941.0
83	794.0
84	647.0
85	457.0
86	267.0
87	182.0
88	97.0
89	82.0
90	67.0
91	52.5
92	38.0
93	31.5
94	25.0
95	26.5
96	28.0
97	18.0
98	8.0
99	8.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008384022003833253
2	4.5494693044056413E-4
3	3.899545118061978E-4
4	0.0
5	0.0
6	1.2998483726873262E-4
7	6.499241863436631E-5
8	6.499241863436631E-5
9	8.44901442246762E-4
10	9.748862795154946E-4
11	0.0
12	0.0
13	5.199393490749305E-4
14	3.899545118061978E-4
15	0.00422450721123381
16	0.001039878698149861
17	0.0
18	2.5996967453746525E-4
19	6.499241863436631E-5
20	0.0011698635354185935
21	0.0
22	1.2998483726873262E-4
23	6.49924186343663E-4
24	3.249620931718315E-4
25	0.0021447498149340877
26	0.0049394238162118395
27	0.0059793025143617
28	0.004419484467136909
29	0.0024697119081059198
30	0.0035095906062557803
31	0.0072141584684146595
32	0.0029246588385464837
33	0.003964537536696344
34	0.0024697119081059198
35	0.0029246588385464837
36	0.007669105398855224
37	0.003249620931718315
38	0.007084173631145926
39	0.004354492048502542
40	0.0015598180472247913
41	0.00298965125718085
42	0.0019497725590309893
43	8.44901442246762E-4
44	0.0011698635354185935
45	0.0011048711167842271
46	7.799090236123956E-4
47	0.0020147649776653554
48	0.0022097422335684543
49	2.5996967453746525E-4
50	0.0011048711167842271
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1538641.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.877843581542656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.74600534453666	46.552631471099446
2	13.371703332616445	16.013375211384
3	4.094682290999323	7.355422372097105
4	1.665111133075014	3.988130558886037
5	0.8798662466924361	2.6342246746064357
6	0.5218010549014142	1.8746593167642496
7	0.36014670191822024	1.5095365518707362
8	0.2340946770482288	1.1213667564452472
9	0.18079501662365924	0.9743054153142484
>10	0.8472454199744123	8.765510248619382
>50	0.04952470423173369	2.0893991134720746
>100	0.04497534245064495	5.384529102737692
>500	0.0036110338580861716	1.4576566117745486
>1k	4.3770107370741477E-4	0.279252594928895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.949772559030989E-4
2	0.0	0.0	0.0	0.0	1.949772559030989E-4
3	0.0	0.0	0.0	0.0	1.949772559030989E-4
4	0.0	0.0	0.0	0.0	1.949772559030989E-4
5	0.0	0.0	0.0	0.0	1.949772559030989E-4
6	0.0	0.0	0.0	0.0	1.949772559030989E-4
7	0.0	0.0	0.0	0.0	1.949772559030989E-4
8	0.0	0.0	0.0	0.0	1.949772559030989E-4
9	0.0	0.0	0.0	6.49924186343663E-5	1.949772559030989E-4
10	0.0	0.0	0.0	6.49924186343663E-5	1.949772559030989E-4
11	0.0	0.0	0.0	6.49924186343663E-5	1.949772559030989E-4
12	0.0	0.0	0.0	6.49924186343663E-5	3.249620931718315E-4
13	0.0	0.0	0.0	6.49924186343663E-5	3.899545118061978E-4
14	0.0	0.0	0.0	6.49924186343663E-5	3.899545118061978E-4
15	0.0	0.0	0.0	6.49924186343663E-5	4.5494693044056413E-4
16	0.0	0.0	0.0	6.49924186343663E-5	4.5494693044056413E-4
17	0.0	0.0	0.0	1.299848372687326E-4	4.5494693044056413E-4
18	6.49924186343663E-5	0.0	0.0	1.299848372687326E-4	5.199393490749304E-4
19	6.49924186343663E-5	0.0	0.0	1.949772559030989E-4	5.199393490749304E-4
20	6.49924186343663E-5	0.0	0.0	1.949772559030989E-4	5.849317677092968E-4
21	6.49924186343663E-5	0.0	0.0	3.899545118061978E-4	5.849317677092968E-4
22	1.299848372687326E-4	0.0	0.0	6.49924186343663E-4	7.149166049780293E-4
23	1.299848372687326E-4	0.0	0.0	0.0014298332099560585	7.149166049780293E-4
24	1.299848372687326E-4	0.0	0.0	0.002144749814934088	7.149166049780293E-4
25	1.299848372687326E-4	0.0	0.0	0.002534704326740286	7.149166049780293E-4
26	1.299848372687326E-4	0.0	0.0	0.0029246588385464837	7.799090236123956E-4
27	1.299848372687326E-4	0.0	0.0	0.003899545118061978	7.799090236123956E-4
28	1.299848372687326E-4	0.0	0.0	0.007604112980220857	7.799090236123956E-4
29	2.599696745374652E-4	0.0	0.0	0.015858150146785377	7.799090236123956E-4
30	2.599696745374652E-4	0.0	0.0	0.030871398851323992	7.799090236123956E-4
31	2.599696745374652E-4	0.0	0.0	0.06057293416722939	8.449014422467619E-4
32	2.599696745374652E-4	0.0	0.0	0.09462896153163733	8.449014422467619E-4
33	3.249620931718315E-4	0.0	0.0	0.12485043619661766	8.449014422467619E-4
34	3.249620931718315E-4	0.0	0.0	0.15936141049146618	8.449014422467619E-4
35	3.249620931718315E-4	0.0	0.0	0.20082657358019187	8.449014422467619E-4
36	3.249620931718315E-4	0.0	0.0	0.2591897655138528	8.449014422467619E-4
37	3.249620931718315E-4	0.0	0.0	0.34725449276341913	8.449014422467619E-4
38	3.249620931718315E-4	0.0	0.0	0.46820538384197485	9.748862795154945E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGG	45	0.0013994164	24.441357	3
CTAGATA	295	0.0	20.87872	3
ATACCGT	280	0.0	20.425991	6
GTATCAA	2595	0.0	19.836933	1
TCGCACG	330	0.0	19.332775	22
TACCGTC	300	0.0	19.06426	7
ACCGTCG	290	0.0	18.963123	8
CCGATCG	315	0.0	18.854761	18
TAGGACG	190	1.8189894E-12	18.523975	4
CGTCGTA	310	0.0	18.451681	10
GATCGCA	335	0.0	18.38753	20
ATCGCAC	335	0.0	18.38753	21
TCTAGAT	365	0.0	18.079908	2
ATAGCGT	235	0.0	17.784988	6
TAGATAA	350	0.0	17.597778	4
GCCGATC	370	0.0	17.241066	17
CCGTCGT	325	0.0	16.922588	9
CGTATAT	225	1.8189894E-12	16.622282	10
CGATCGC	360	0.0	16.498451	19
CGCAATA	325	0.0	16.249384	36
>>END_MODULE