FastQCFastQC Report
Thu 2 Feb 2017
SRR4064201_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064201_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180684
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45350.38409938645734165No Hit
TATCAACGCAGAGTACTTTTTTTTT36530.3093969258497617No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31150.26383011881248497No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22850.19353188490739268No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21310.1804885981346406No Hit
GTACATGGGGTGGTATCAACGCAAA16740.14178222115316205No Hit
GGTATCAACGCAGAGTACTTTTTTT16240.13754738778538542No Hit
GTATCAACGCAGAGTACATGGGGTG12970.10985157756012616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC953.0377123E-1015.00147217
CGACGAC400.00527467214.25139814
CGCATCG801.2866258E-714.25079313
AAAGGCG759.679443E-713.9317475
ATTTGCG1051.366061E-913.57333616
GCACGCG500.001499501513.29961611
CTGTGCG500.001499501513.2996169
GCGTTAT801.9988893E-613.062121
TTGCGCG1051.9876097E-812.66844718
TCGTTAA604.087343E-412.6679112
GTTCCGG751.4783103E-512.6662999
TCCAACG1700.012.29584518
CGGTCCA1405.2750693E-1112.21393210
TAGATCT701.0916294E-412.2128974
GACCGTT550.003069333712.0900477
AAGGCGT802.8670847E-511.8741526
ATTACCG1052.712568E-711.76355715
GAATTAG658.0228597E-411.6919691
TCGCCAG907.4480486E-611.6122517
GCAAGAC1900.011.4982063
Produced by FastQC (version 0.11.5)