##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064201_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1180684
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.191699049025818	32.0	32.0	32.0	32.0	32.0
2	31.001155262542728	32.0	32.0	32.0	32.0	32.0
3	31.013960551680213	32.0	32.0	32.0	32.0	32.0
4	31.02073289720196	32.0	32.0	32.0	32.0	32.0
5	31.032533683864607	32.0	32.0	32.0	32.0	32.0
6	34.57767107879839	36.0	36.0	36.0	32.0	36.0
7	34.50622520505063	36.0	36.0	36.0	32.0	36.0
8	34.46957441618587	36.0	36.0	36.0	32.0	36.0
9	34.53039339908053	36.0	36.0	36.0	32.0	36.0
10	34.39707576286288	36.0	36.0	36.0	32.0	36.0
11	34.5457582215055	36.0	36.0	36.0	32.0	36.0
12	34.43663249438461	36.0	36.0	36.0	32.0	36.0
13	34.47324771064908	36.0	36.0	36.0	32.0	36.0
14	34.41359330693056	36.0	36.0	36.0	32.0	36.0
15	34.387910736488344	36.0	36.0	36.0	32.0	36.0
16	34.39255888959281	36.0	36.0	36.0	32.0	36.0
17	34.34339586205962	36.0	36.0	36.0	32.0	36.0
18	34.336273719301694	36.0	36.0	36.0	32.0	36.0
19	34.31056489289259	36.0	36.0	36.0	32.0	36.0
20	34.262539341601986	36.0	36.0	36.0	32.0	36.0
21	34.22929759359829	36.0	36.0	36.0	32.0	36.0
22	34.23463094274167	36.0	36.0	36.0	32.0	36.0
23	34.20362179888946	36.0	36.0	36.0	32.0	36.0
24	34.18927164253941	36.0	36.0	36.0	32.0	36.0
25	33.69814531237825	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	11.0
5	22.0
6	44.0
7	14.0
8	63.0
9	87.0
10	106.0
11	40.0
12	79.0
13	69.0
14	182.0
15	279.0
16	537.0
17	662.0
18	813.0
19	1006.0
20	1406.0
21	1889.0
22	2536.0
23	3556.0
24	5066.0
25	7281.0
26	10327.0
27	13840.0
28	19507.0
29	26782.0
30	36698.0
31	52179.0
32	77647.0
33	116109.0
34	267959.0
35	533885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.504504603736365	18.007061293621536	11.611566151544139	24.876867951097957
2	15.168321863419798	20.65262043409211	40.437226045526735	23.741831656961352
3	18.84331091937327	25.752987939827026	29.155059487762387	26.248641653037318
4	11.247939666314878	16.17986607195795	38.46196196280981	34.11023229891736
5	12.670255875098258	38.819355975388284	34.8790759764725	13.631312173040957
6	31.474069499822537	37.9351997973776	18.156474980114712	12.434255722685153
7	28.171324103740574	31.762291957174053	21.821390267416465	18.2449936716689
8	26.72571560261094	35.02555360495618	20.093894247388718	18.15483654504416
9	27.276864059710242	14.04551652022415	19.411337523377277	39.26628189668833
10	14.312506303024858	28.728318653817958	33.88370569556157	23.07546934759561
11	36.02580323676046	21.87283634758099	23.830282362731747	18.271078052926804
12	23.469355755971588	24.979341101926554	31.473566357228155	20.077736784873704
13	29.20740177594482	21.071652262647273	26.161496221222684	23.559449740185222
14	22.0137881232964	21.202417922187113	27.743131365183547	29.040662589332932
15	23.684529454488747	29.5089830914988	24.145570237105478	22.660917216906977
16	23.17130122072874	27.253279813267405	26.877729646257336	22.697689319746512
17	21.684321863317503	27.19843853423588	27.4166742236954	23.700565378751218
18	22.06856916167817	26.985438843651938	29.140876517528824	21.805115477141072
19	23.77881887434424	25.91276545066324	27.731218515281768	22.57719715971075
20	24.168400137660484	26.11463456397059	27.495901549019507	22.22106374934942
21	25.429215380299024	25.329111037838764	26.273194159195572	22.96847942266664
22	23.763060236020905	26.502933732069987	27.292354326343577	22.44165170556553
23	23.106853068130572	26.308494874863946	27.33106378361516	23.25358827339032
24	22.98457438422142	26.677126722645767	27.80043840688431	22.5378604862485
25	23.163331301796767	26.622170647376393	27.670460469378188	22.544037581448652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	149.0
1	149.0
2	458.0
3	767.0
4	767.0
5	767.0
6	2478.0
7	4189.0
8	4189.0
9	4189.0
10	4295.5
11	4402.0
12	4402.0
13	4402.0
14	4639.0
15	4876.0
16	4876.0
17	4876.0
18	8574.5
19	12273.0
20	12273.0
21	12273.0
22	19847.5
23	27422.0
24	27422.0
25	27422.0
26	39875.5
27	52329.0
28	52329.0
29	52329.0
30	66792.0
31	81255.0
32	81255.0
33	81255.0
34	90368.5
35	99482.0
36	99482.0
37	99482.0
38	107274.5
39	115067.0
40	115067.0
41	115067.0
42	125492.0
43	135917.0
44	135917.0
45	135917.0
46	146650.0
47	157383.0
48	157383.0
49	157383.0
50	154139.0
51	150895.0
52	150895.0
53	150895.0
54	132555.0
55	114215.0
56	114215.0
57	114215.0
58	101514.5
59	88814.0
60	88814.0
61	88814.0
62	74754.0
63	60694.0
64	60694.0
65	60694.0
66	48473.0
67	36252.0
68	36252.0
69	36252.0
70	26644.5
71	17037.0
72	17037.0
73	17037.0
74	12921.0
75	8805.0
76	8805.0
77	8805.0
78	6856.5
79	4908.0
80	4908.0
81	4908.0
82	3290.5
83	1673.0
84	1673.0
85	1673.0
86	1284.5
87	896.0
88	896.0
89	896.0
90	601.5
91	307.0
92	307.0
93	307.0
94	225.0
95	143.0
96	143.0
97	143.0
98	338.5
99	534.0
100	534.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037859410307923204
2	0.004997103373976441
3	0.0017786300144661906
4	0.0042348333677766446
5	0.009147240074397553
6	0.013805556778951863
7	0.023545673524838145
8	0.03557260028932382
9	0.049547550402986744
10	0.05886418381209536
11	0.05733964379969577
12	0.06818081722120398
13	0.06818081722120398
14	0.07012884057038124
15	0.06309901717987201
16	0.06902778389475932
17	0.06496234386169372
18	0.0821557673348669
19	0.08003835065097858
20	0.0821557673348669
21	0.07749745063031259
22	0.08113940732660051
23	0.08537424069437716
24	0.08113940732660051
25	0.08071592398982284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1180684.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.803411403480375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.44335165753981	33.402690487759955
2	16.81620212716132	16.41376061311871
3	6.72063928689753	9.839703720385593
4	3.132223831931069	6.1145283311007095
5	1.643531041109013	4.01049607768168
6	0.9392669561396548	2.7503659006906997
7	0.5838101273919433	1.9944348080039107
8	0.37601258528766174	1.4680557514144008
9	0.2563170200897885	1.1258230483040483
>10	0.9140133550543947	7.800680521025251
>50	0.08611964493970348	2.979186280859543
>100	0.07892933825010791	7.546048142650452
>500	0.007840659442791971	2.640036305763028
>1k	0.0017423687650648823	1.9141900112420371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4535	0.38409938645734165	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3653	0.3093969258497617	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3115	0.26383011881248497	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2285	0.19353188490739268	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2131	0.1804885981346406	No Hit
GTACATGGGGTGGTATCAACGCAAA	1674	0.14178222115316205	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1624	0.13754738778538542	No Hit
GTATCAACGCAGAGTACATGGGGTG	1297	0.10985157756012616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGCGC	95	3.0377123E-10	15.001472	17
CGACGAC	40	0.005274672	14.251398	14
CGCATCG	80	1.2866258E-7	14.250793	13
AAAGGCG	75	9.679443E-7	13.931747	5
ATTTGCG	105	1.366061E-9	13.573336	16
GCACGCG	50	0.0014995015	13.299616	11
CTGTGCG	50	0.0014995015	13.299616	9
GCGTTAT	80	1.9988893E-6	13.06212	1
TTGCGCG	105	1.9876097E-8	12.668447	18
TCGTTAA	60	4.087343E-4	12.66791	12
GTTCCGG	75	1.4783103E-5	12.666299	9
TCCAACG	170	0.0	12.295845	18
CGGTCCA	140	5.2750693E-11	12.213932	10
TAGATCT	70	1.0916294E-4	12.212897	4
GACCGTT	55	0.0030693337	12.090047	7
AAGGCGT	80	2.8670847E-5	11.874152	6
ATTACCG	105	2.712568E-7	11.763557	15
GAATTAG	65	8.0228597E-4	11.691969	1
TCGCCAG	90	7.4480486E-6	11.61225	17
GCAAGAC	190	0.0	11.498206	3
>>END_MODULE