FastQCFastQC Report
Thu 2 Feb 2017
SRR4064201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180684
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14000.11857533429774605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12230.10358402417581672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC6300.020.9515951
TAGGACG4900.020.6522874
TATACCG654.9333175E-420.306935
GTTCCGT550.00448452619.99924930
GTAGGAC7900.019.771413
ATCACGG3350.019.70325735
TAGGACC7800.019.4608084
GTCCTAC7350.019.4550531
GATTTCG2950.019.39074341
TGTAGGA8450.019.00442
AGGACGT5350.018.9151765
AAGCGTA708.125222E-418.8564365
ATTTCGT3150.018.85643642
AATCACG3650.018.68502234
ATTCGTG951.5964102E-518.5271944
TCGTGAA951.5975755E-518.5256216
CTGTAGG8200.018.51151
AAGGCGT1557.221388E-1018.450926
GTAAGCG600.007413607618.3326453
ATACACT7250.018.2062134
Produced by FastQC (version 0.11.5)