##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1180684
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.184864874936903	32.0	32.0	32.0	32.0	32.0
2	31.245912538833423	32.0	32.0	32.0	32.0	32.0
3	31.38532579420065	32.0	32.0	32.0	32.0	32.0
4	31.488582889240472	32.0	32.0	32.0	32.0	32.0
5	31.41376524116529	32.0	32.0	32.0	32.0	32.0
6	34.97584874530357	36.0	36.0	36.0	36.0	36.0
7	35.00771163156272	36.0	36.0	36.0	36.0	36.0
8	34.94888640821761	36.0	36.0	36.0	36.0	36.0
9	35.08437905485295	36.0	36.0	36.0	36.0	36.0
10	34.915665834380746	36.0	36.0	36.0	32.0	36.0
11	35.08923471479244	36.0	36.0	36.0	36.0	36.0
12	34.9842582774053	36.0	36.0	36.0	36.0	36.0
13	35.0372419716029	36.0	36.0	36.0	36.0	36.0
14	34.9741725982566	36.0	36.0	36.0	36.0	36.0
15	34.93932500143984	36.0	36.0	36.0	36.0	36.0
16	34.9619347767904	36.0	36.0	36.0	36.0	36.0
17	34.93415172899777	36.0	36.0	36.0	36.0	36.0
18	34.924874903022314	36.0	36.0	36.0	36.0	36.0
19	34.92530939692585	36.0	36.0	36.0	36.0	36.0
20	34.904741658225234	36.0	36.0	36.0	32.0	36.0
21	34.89802775340396	36.0	36.0	36.0	32.0	36.0
22	34.88463043456166	36.0	36.0	36.0	32.0	36.0
23	34.83830305145153	36.0	36.0	36.0	32.0	36.0
24	34.82290519732629	36.0	36.0	36.0	32.0	36.0
25	34.79926635746737	36.0	36.0	36.0	32.0	36.0
26	34.7472456643776	36.0	36.0	36.0	32.0	36.0
27	34.739213879412276	36.0	36.0	36.0	32.0	36.0
28	34.71172472905536	36.0	36.0	36.0	32.0	36.0
29	34.68412208516419	36.0	36.0	36.0	32.0	36.0
30	34.6555081630648	36.0	36.0	36.0	32.0	36.0
31	34.63557564936935	36.0	36.0	36.0	32.0	36.0
32	34.59602823448103	36.0	36.0	36.0	32.0	36.0
33	34.56631494963936	36.0	36.0	36.0	32.0	36.0
34	34.550019310840156	36.0	36.0	36.0	32.0	36.0
35	34.50178964058122	36.0	36.0	36.0	32.0	36.0
36	34.46394716960677	36.0	36.0	36.0	32.0	36.0
37	34.44693330306839	36.0	36.0	36.0	32.0	36.0
38	34.388014913389185	36.0	36.0	36.0	32.0	36.0
39	34.371695559523125	36.0	36.0	36.0	32.0	36.0
40	34.36112795633717	36.0	36.0	36.0	32.0	36.0
41	34.334985482991215	36.0	36.0	36.0	32.0	36.0
42	34.26106816049002	36.0	36.0	36.0	32.0	36.0
43	34.250351491169525	36.0	36.0	36.0	32.0	36.0
44	34.17292349180644	36.0	36.0	36.0	32.0	36.0
45	34.12133305778684	36.0	36.0	36.0	32.0	36.0
46	34.06535957123159	36.0	36.0	36.0	32.0	36.0
47	34.03296902473482	36.0	36.0	36.0	32.0	36.0
48	33.95612712630983	36.0	36.0	36.0	32.0	36.0
49	33.9238890338143	36.0	36.0	36.0	32.0	36.0
50	33.30996947532108	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	0.0
19	2.0
20	9.0
21	20.0
22	87.0
23	309.0
24	879.0
25	2155.0
26	4465.0
27	8314.0
28	14187.0
29	22396.0
30	33600.0
31	48159.0
32	70055.0
33	113896.0
34	228940.0
35	633209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50529994629749	18.975959233527984	12.168700416236792	25.350040403937733
2	14.479029034115934	21.562658806789308	39.68797642036792	24.270335738726835
3	18.11979696463312	26.217665906370986	29.573695181001476	26.088841947994418
4	11.01886702962012	16.945262237821463	38.53800000677573	33.49787072578268
5	12.399338010847949	39.32220644982061	34.445457040156384	13.832998499175053
6	31.72810291001303	38.27542047731735	17.789040571466323	12.207436041203303
7	28.031486859724414	31.952437699196146	21.738265055057116	18.277810386022328
8	26.73407956743718	35.26735350017447	19.846038398081113	18.152528534307233
9	27.163852450130094	13.821789624891586	19.201861312879444	39.812496612098876
10	14.171796496244523	29.103920127113618	33.86153356212881	22.86274981451304
11	36.2971802785504	21.715971419956567	23.711594296187634	18.2752540053054
12	23.49798421248772	25.119422705559508	31.296032794660704	20.08656028729207
13	29.335323148798274	21.061694160732934	26.165282858422994	23.437699832045798
14	22.057421049867788	21.08001985293246	27.780215163778223	29.082343933421527
15	23.75398412533044	29.540674711571775	24.00690144101885	22.698439722078938
16	23.361825533551176	27.241389240383036	26.629535333207983	22.76724989285781
17	21.716818386630123	27.280203678545657	27.353974475812326	23.649003459011894
18	22.236046623900425	26.950948688978066	29.095810726342908	21.717193960778598
19	23.918209983721272	25.82126262617707	27.75201112577307	22.50851626432858
20	24.293435037694476	26.116287519787377	27.39436780762224	22.195909634895912
21	25.447537190306633	25.289323815686497	26.37174722449021	22.891391769516652
22	23.781993242871263	26.427250759944066	27.291414516562078	22.499341480622594
23	23.194229054107275	26.194890541242156	27.388277702632312	23.222602702018253
24	23.05738407488591	26.566814265725736	27.765280229292372	22.61052143009598
25	23.342932402788247	26.44879602259734	27.69334360998416	22.514927964630253
26	22.192341702890324	27.28353046006024	28.46578261738668	22.058345219662755
27	22.997388662407324	26.732118097170897	27.683058322696276	22.587434917725503
28	22.193113740756083	27.257904816112603	27.630583725281436	22.91839771784988
29	22.457656906813433	26.42884911993617	27.558224135287844	23.555269837962552
30	22.464358555640633	27.058022374153857	27.753572614360927	22.724046455844586
31	23.17228527867186	26.53133999661189	27.160935117736745	23.135439606979503
32	22.611415605172418	27.280804508036695	27.181452203749302	22.926327683041585
33	22.536573631749775	26.692144546310793	27.70558480542625	23.06569701651319
34	23.057898209531942	26.930794373564872	27.811841194740822	22.199466222162368
35	23.22004526327915	26.982978838739403	27.585444603302594	22.211531294678856
36	22.1537916833602	27.446537641549146	27.568848641831213	22.830822033259444
37	23.144852680847748	27.056903324823782	26.959836833430174	22.838407160898296
38	22.576093051463925	27.373169777247725	27.187161716898444	22.86357545438991
39	23.288792066595065	26.847914838543556	26.921180930826644	22.942112164034732
40	23.78459002702746	27.027038472842523	27.237939069208426	21.950432430921584
41	22.809655135028915	26.997482712707388	27.906230201349103	22.286631950914593
42	23.38310775329053	27.897023698609253	26.9045279758779	21.815340572222315
43	22.45516082475624	27.332924523827266	27.514178462289017	22.697736189127475
44	22.36303716458578	27.73025755780419	26.767411979838514	23.139293297771513
45	22.548356141062637	27.754819944997195	26.865661528610524	22.831162385329648
46	22.6557689457302	27.035043513244545	26.627225951256694	23.681961589768566
47	23.099035796805865	27.018016980275505	26.73825914908899	23.14468807382964
48	23.149399021053533	27.282606209932265	26.243608431576654	23.324386337437545
49	22.911288410068774	27.718094657316122	26.288748856591116	23.081868076023987
50	22.840914673293458	27.837179480664236	26.00117561164838	23.320730234393924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	19.5
2	15.0
3	78.0
4	141.0
5	195.5
6	250.0
7	269.5
8	289.0
9	399.0
10	509.0
11	773.0
12	1037.0
13	1971.5
14	2906.0
15	3934.5
16	4963.0
17	5737.5
18	6512.0
19	6854.0
20	7196.0
21	7950.5
22	8705.0
23	9753.5
24	10802.0
25	12343.0
26	13884.0
27	17831.0
28	21778.0
29	25050.0
30	28322.0
31	31716.5
32	35111.0
33	40719.0
34	46327.0
35	51500.0
36	56673.0
37	61195.5
38	65718.0
39	66260.5
40	66803.0
41	69258.5
42	71714.0
43	72009.0
44	72304.0
45	75215.5
46	78127.0
47	80258.0
48	82389.0
49	83101.5
50	83814.0
51	79765.5
52	75717.0
53	72384.5
54	69052.0
55	67031.5
56	65011.0
57	61381.5
58	57752.0
59	51356.5
60	44961.0
61	38785.5
62	32610.0
63	27903.0
64	23196.0
65	19439.0
66	15682.0
67	13261.5
68	10841.0
69	9451.5
70	8062.0
71	6000.5
72	3939.0
73	3402.5
74	2866.0
75	2238.0
76	1610.0
77	1432.5
78	1255.0
79	1010.5
80	766.0
81	602.5
82	439.0
83	360.5
84	282.0
85	216.5
86	151.0
87	114.0
88	77.0
89	54.0
90	31.0
91	29.0
92	27.0
93	19.5
94	12.0
95	14.5
96	17.0
97	11.5
98	6.0
99	7.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008977846739686487
2	3.3878666942213154E-4
3	2.540900020665987E-4
4	0.0
5	0.0
6	1.6939333471106577E-4
7	8.469666735553289E-5
8	0.0
9	0.0010163600082663947
10	6.775733388442631E-4
11	0.0
12	3.3878666942213154E-4
13	4.2348333677766446E-4
14	1.6939333471106577E-4
15	0.005166496708687507
16	8.469666735553289E-4
17	0.0
18	1.6939333471106577E-4
19	1.6939333471106577E-4
20	5.928766714887303E-4
21	0.0
22	4.2348333677766446E-4
23	9.316633409108618E-4
24	6.775733388442631E-4
25	0.0011857533429774606
26	0.004743013371909842
27	0.005335890043398572
28	0.0036419566962879145
29	0.0026255966880215195
30	0.0030490800247991844
31	0.0071145200578647625
32	0.0031337766921547172
33	0.004404226702487711
34	0.0036419566962879145
35	0.0030490800247991844
36	0.007368610059931361
37	0.0042348333677766446
38	0.00762270006199796
39	0.004743013371909842
40	0.002794990022732585
41	0.004065440033065579
42	0.0020327200165327895
43	0.0016939333471106578
44	0.0014398433450440592
45	0.0014398433450440592
46	7.622700061997961E-4
47	0.0016939333471106578
48	0.0022868100185993883
49	3.3878666942213154E-4
50	0.0018633266818217236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1180684.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.36140152794419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.27721005654028	37.50090424090723
2	16.215033272908308	17.305138025292706
3	6.256049536789966	10.014947139340759
4	2.8822264649461533	6.151985747618354
5	1.4926690323586145	3.9825455792007975
6	0.8572761262384089	2.7447273355516986
7	0.5331034346990982	1.9913002504433328
8	0.33425069920841943	1.4268868617165253
9	0.21938680957146023	1.0536108871929586
>10	0.7979930300876802	7.136216246031407
>50	0.07014667733391619	2.6465102993405254
>100	0.05954633226906265	6.138743984474646
>500	0.004789244108090668	1.6830380961072064
>1k	3.192829405393778E-4	0.22344530678185975
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1400	0.11857533429774605	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1223	0.10358402417581672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	8.46966673555329E-5	0.0
11	0.0	0.0	0.0	8.46966673555329E-5	0.0
12	0.0	0.0	0.0	8.46966673555329E-5	0.0
13	0.0	0.0	0.0	8.46966673555329E-5	0.0
14	0.0	0.0	0.0	8.46966673555329E-5	0.0
15	0.0	0.0	0.0	8.46966673555329E-5	0.0
16	0.0	0.0	0.0	8.46966673555329E-5	0.0
17	0.0	0.0	0.0	8.46966673555329E-5	0.0
18	0.0	0.0	0.0	8.46966673555329E-5	0.0
19	0.0	0.0	0.0	8.46966673555329E-5	0.0
20	0.0	0.0	0.0	8.46966673555329E-5	0.0
21	0.0	0.0	0.0	8.46966673555329E-5	0.0
22	0.0	0.0	0.0	1.693933347110658E-4	0.0
23	0.0	0.0	0.0	4.2348333677766446E-4	0.0
24	0.0	0.0	0.0	6.775733388442632E-4	0.0
25	0.0	0.0	0.0	0.0011857533429774606	0.0
26	0.0	0.0	0.0	0.0012704500103329934	0.0
27	0.0	0.0	0.0	0.0014398433450440592	0.0
28	0.0	0.0	0.0	0.0022868100185993883	0.0
29	0.0	0.0	0.0	0.00440422670248771	0.0
30	0.0	0.0	0.0	0.007961486731420091	0.0
31	0.0	0.0	0.0	0.016769940136395513	0.0
32	0.0	0.0	0.0	0.026086573545504133	0.0
33	0.0	0.0	0.0	0.03396336360956869	0.0
34	0.0	0.0	0.0	0.04344939035338837	0.0
35	0.0	0.0	0.0	0.056662070460851505	0.0
36	0.0	0.0	0.0	0.07470246060758001	0.0
37	0.0	0.0	0.0	0.10383811417788333	0.0
38	0.0	0.0	0.0	0.14762629120069384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	630	0.0	20.951595	1
TAGGACG	490	0.0	20.652287	4
TATACCG	65	4.9333175E-4	20.30693	5
GTTCCGT	55	0.004484526	19.999249	30
GTAGGAC	790	0.0	19.77141	3
ATCACGG	335	0.0	19.703257	35
TAGGACC	780	0.0	19.460808	4
GTCCTAC	735	0.0	19.455053	1
GATTTCG	295	0.0	19.390743	41
TGTAGGA	845	0.0	19.0044	2
AGGACGT	535	0.0	18.915176	5
AAGCGTA	70	8.125222E-4	18.856436	5
ATTTCGT	315	0.0	18.856436	42
AATCACG	365	0.0	18.685022	34
ATTCGTG	95	1.5964102E-5	18.52719	44
TCGTGAA	95	1.5975755E-5	18.52562	16
CTGTAGG	820	0.0	18.5115	1
AAGGCGT	155	7.221388E-10	18.45092	6
GTAAGCG	60	0.0074136076	18.332645	3
ATACACT	725	0.0	18.206213	4
>>END_MODULE