FastQCFastQC Report
Thu 2 Feb 2017
SRR4064200_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064200_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671227
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51120.30588304281824075No Hit
TATCAACGCAGAGTACTTTTTTTTT35380.2117007444231095No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23160.13858081517352222No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20990.1255963432855022No Hit
GAATAGGACCGCGGTTCTATTTTGT19800.11847582644368478No Hit
CTATTGGAGCTGGAATTACCGCGGC19430.1162618842323634No Hit
GATTAAGAGGGACGGCCGGGGGCAT18940.11332990670926212No Hit
GGTATCAACGCAGAGTACTTTTTTT18350.10979956642634425No Hit
GTACATGGGGAATAATTGCAATCCC17340.10375610255219667No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17310.1035765937242517No Hit
GTATCTGATCGTCTTCGAACCTCCG17290.10345692117228837No Hit
GTCCTATTCCATTATTCCTAGCTGC16890.10106347013302203No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16770.10034543482124211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA400.005272753514.25267313
CCCTTAC852.7169335E-713.4046431
GTATTAG3500.013.2929391
GTATCGC604.096174E-412.665256
CTAGACA1352.7284841E-1112.6629744
ATTAGCT1850.012.3188883
ATCCCCG2800.012.21657812
TGCACCG1251.4151738E-912.157915
GCGTAAC1103.8073267E-812.09245311
ACCGTGT1052.7156239E-711.7634068
CCGTCGT3250.011.6938019
CTATTAG658.0597994E-411.68611
ACGCGCG907.445813E-611.6132913
CGCGTAA1157.064227E-811.56634810
AAGCTCG1157.064227E-811.5663487
CGTCGTA3450.011.56634710
CTTACAG1157.117342E-811.5601163
AGGCCCG5000.011.401113510
GGATAAG1001.9420386E-611.3939481
CGGCGTC2450.011.24667114
Produced by FastQC (version 0.11.5)