##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064200_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1671227
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248515013220825	32.0	32.0	32.0	32.0	32.0
2	30.841105965856226	32.0	32.0	32.0	32.0	32.0
3	30.8589916271099	32.0	32.0	32.0	32.0	32.0
4	30.89165385671725	32.0	32.0	32.0	32.0	32.0
5	30.783942576322666	32.0	32.0	32.0	32.0	32.0
6	34.44155222480249	36.0	36.0	36.0	32.0	36.0
7	34.382311918129616	36.0	36.0	36.0	32.0	36.0
8	34.33365904212893	36.0	36.0	36.0	32.0	36.0
9	34.48736287769405	36.0	36.0	36.0	32.0	36.0
10	34.165836238883166	36.0	36.0	36.0	32.0	36.0
11	34.47792131170691	36.0	36.0	36.0	32.0	36.0
12	34.278697029188734	36.0	36.0	36.0	32.0	36.0
13	34.367585013885005	36.0	36.0	36.0	32.0	36.0
14	34.26350100854043	36.0	36.0	36.0	32.0	36.0
15	34.18808336629315	36.0	36.0	36.0	32.0	36.0
16	34.18844836757663	36.0	36.0	36.0	32.0	36.0
17	34.11197042651896	36.0	36.0	36.0	32.0	36.0
18	34.11291344622843	36.0	36.0	36.0	32.0	36.0
19	34.13493259742692	36.0	36.0	36.0	32.0	36.0
20	34.099397628209694	36.0	36.0	36.0	32.0	36.0
21	34.08066289019983	36.0	36.0	36.0	32.0	36.0
22	34.0696248923695	36.0	36.0	36.0	32.0	36.0
23	34.01594756427463	36.0	36.0	36.0	32.0	36.0
24	33.99798411586218	36.0	36.0	36.0	32.0	36.0
25	33.59392589995255	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	13.0
5	16.0
6	63.0
7	22.0
8	95.0
9	103.0
10	146.0
11	48.0
12	98.0
13	86.0
14	160.0
15	253.0
16	489.0
17	618.0
18	833.0
19	1150.0
20	1628.0
21	2562.0
22	3892.0
23	5937.0
24	8835.0
25	13219.0
26	18402.0
27	24418.0
28	33706.0
29	45646.0
30	59383.0
31	81643.0
32	116076.0
33	165813.0
34	352718.0
35	733151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05542446477249	16.819127043130663	11.268001039148857	25.857447452947984
2	17.058901320108117	19.501529185573297	36.819114490551726	26.620455003766864
3	18.56916329082869	23.891418689676218	28.390787402988288	29.1486306165068
4	12.61148753164735	15.038018706867303	35.58882470824001	36.76166905324534
5	14.821842866982509	36.53174202806447	33.06302926070011	15.583385844252911
6	34.54912789062397	35.159299248476664	16.76636708665319	13.525205774246174
7	30.81914506506813	30.249858755709617	20.556251137136236	18.374745042086012
8	28.980916501163012	31.79148574992608	19.103431103194072	20.124166645716837
9	28.008960357839623	13.546075982254086	18.183097082316404	40.26186657758989
10	16.152185951835722	26.05588732731654	30.899076527023183	26.892850193824554
11	37.46674361633089	20.740249383963658	22.391633879244903	19.401373120460548
12	25.09413323482448	23.32863739859984	28.34055485472438	23.236674511851298
13	29.566476809050897	19.004801224285252	25.27351531164849	26.155206655015363
14	24.23169405712479	19.12849485800231	24.890250561620146	31.74956052325275
15	25.5707453636294	26.508077726262545	22.216740746417347	25.7044361636907
16	26.241810772718182	25.200008142825155	23.24477212663051	25.313408957826155
17	24.315084691269472	25.344815095767505	24.831716103584927	25.508384109378095
18	25.253309141644138	24.32372552013629	25.74067587028704	24.682289467932538
19	26.03416684630308	24.29396900673038	25.10526693971402	24.56659720725252
20	26.261445571744147	23.44229144373339	24.570396216935762	25.7258667675867
21	27.52134101066811	23.426789076085132	24.069594209424537	24.98227570382222
22	26.57367154097654	23.329141666367263	24.54981496784393	25.54737182481227
23	24.730583960313982	23.34350155816967	25.283488671232185	26.64242581028416
24	25.204324512055283	24.43558890189609	24.814807052631263	25.545279533417364
25	25.21790314776935	23.734137800537482	25.03916148904676	26.008797562646407
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	166.0
2	414.0
3	662.0
4	662.0
5	662.0
6	1864.0
7	3066.0
8	3066.0
9	3066.0
10	3078.5
11	3091.0
12	3091.0
13	3091.0
14	3258.0
15	3425.0
16	3425.0
17	3425.0
18	6154.5
19	8884.0
20	8884.0
21	8884.0
22	14214.0
23	19544.0
24	19544.0
25	19544.0
26	29908.0
27	40272.0
28	40272.0
29	40272.0
30	50775.5
31	61279.0
32	61279.0
33	61279.0
34	77641.0
35	94003.0
36	94003.0
37	94003.0
38	110653.0
39	127303.0
40	127303.0
41	127303.0
42	149571.5
43	171840.0
44	171840.0
45	171840.0
46	195187.5
47	218535.0
48	218535.0
49	218535.0
50	225758.5
51	232982.0
52	232982.0
53	232982.0
54	217244.0
55	201506.0
56	201506.0
57	201506.0
58	186220.0
59	170934.0
60	170934.0
61	170934.0
62	152645.5
63	134357.0
64	134357.0
65	134357.0
66	110850.0
67	87343.0
68	87343.0
69	87343.0
70	65492.0
71	43641.0
72	43641.0
73	43641.0
74	33610.5
75	23580.0
76	23580.0
77	23580.0
78	18849.5
79	14119.0
80	14119.0
81	14119.0
82	9665.0
83	5211.0
84	5211.0
85	5211.0
86	4010.0
87	2809.0
88	2809.0
89	2809.0
90	2014.0
91	1219.0
92	1219.0
93	1219.0
94	859.0
95	499.0
96	499.0
97	499.0
98	728.0
99	957.0
100	957.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0376370175924635
2	0.004547556974606083
3	0.0022139422113213823
4	0.004547556974606083
5	0.008736096293322212
6	0.013223816991946635
7	0.02172056818134221
8	0.034046841033563965
9	0.04535589719409751
10	0.053972320935456404
11	0.05211739638002498
12	0.05911824066987907
13	0.06151169170914544
14	0.06336661626457686
15	0.05714364356248433
16	0.06252890840083364
17	0.05887889556595244
18	0.07371829200940387
19	0.07102565959022922
20	0.075274035184927
21	0.07198304000593576
22	0.07461583614912876
23	0.07892404801980821
24	0.07252156648977069
25	0.0729404204216423
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1671227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.52761616493543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.17159293008804	30.267581859778165
2	14.880510258666716	12.656652572379242
3	5.52020909875493	7.042840011061015
4	2.566499686970258	4.365884542995923
5	1.482269059025701	3.151868479770252
6	0.8986730769205888	2.2931054203824175
7	0.5941890097070183	1.7688609493970215
8	0.4438408927959427	1.5100396101702485
9	0.32784576170207425	1.2548248843470033
>10	1.8598857625713756	14.350181242876026
>50	0.13394974259333134	3.9065139767462136
>100	0.10037530878752457	9.136392591712205
>500	0.013533590881094588	3.94708325277596
>1k	0.006484845630524489	4.041689507300543
>5k	1.4097490501140193E-4	0.3064810983077754
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5112	0.30588304281824075	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3538	0.2117007444231095	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2316	0.13858081517352222	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2099	0.1255963432855022	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1980	0.11847582644368478	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1943	0.1162618842323634	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1894	0.11332990670926212	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1835	0.10979956642634425	No Hit
GTACATGGGGAATAATTGCAATCCC	1734	0.10375610255219667	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1731	0.1035765937242517	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1729	0.10345692117228837	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1689	0.10106347013302203	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1677	0.10034543482124211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1967255196331796E-4
2	0.0	0.0	0.0	0.0	1.1967255196331796E-4
3	0.0	0.0	0.0	0.0	1.1967255196331796E-4
4	0.0	0.0	0.0	0.0	1.1967255196331796E-4
5	0.0	0.0	0.0	0.0	1.1967255196331796E-4
6	0.0	0.0	0.0	0.0	1.1967255196331796E-4
7	0.0	0.0	0.0	0.0	1.1967255196331796E-4
8	0.0	0.0	0.0	0.0	1.1967255196331796E-4
9	0.0	0.0	0.0	0.0	1.1967255196331796E-4
10	0.0	0.0	0.0	0.0	1.7950882794497694E-4
11	0.0	0.0	0.0	0.0	1.7950882794497694E-4
12	0.0	0.0	0.0	0.0	5.983627598165898E-4
13	0.0	0.0	0.0	0.0	7.180353117799078E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGTA	40	0.0052727535	14.252673	13
CCCTTAC	85	2.7169335E-7	13.404643	1
GTATTAG	350	0.0	13.292939	1
GTATCGC	60	4.096174E-4	12.66525	6
CTAGACA	135	2.7284841E-11	12.662974	4
ATTAGCT	185	0.0	12.318888	3
ATCCCCG	280	0.0	12.216578	12
TGCACCG	125	1.4151738E-9	12.15791	5
GCGTAAC	110	3.8073267E-8	12.092453	11
ACCGTGT	105	2.7156239E-7	11.763406	8
CCGTCGT	325	0.0	11.693801	9
CTATTAG	65	8.0597994E-4	11.6861	1
ACGCGCG	90	7.445813E-6	11.61329	13
CGCGTAA	115	7.064227E-8	11.566348	10
AAGCTCG	115	7.064227E-8	11.566348	7
CGTCGTA	345	0.0	11.566347	10
CTTACAG	115	7.117342E-8	11.560116	3
AGGCCCG	500	0.0	11.4011135	10
GGATAAG	100	1.9420386E-6	11.393948	1
CGGCGTC	245	0.0	11.246671	14
>>END_MODULE