FastQCFastQC Report
Thu 2 Feb 2017
SRR4064200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671227
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20550.12296354714230921No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19430.1162618842323634No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19210.11494548616076691No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA18060.10806431442287613No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC17320.10363643000023337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35650.019.9317421
GTATAGA1553.45608E-1119.8700351
GTTATAT1404.087269E-918.8562581
ACCGTCG5000.018.4796878
ATACCGT5250.018.0187426
TACGCTC750.00129052117.6012834
TACCGTC5600.017.2854217
CGGTCCA6800.016.8227410
CGCAATA6550.016.79511636
GTATATA2007.2759576E-1116.4992261
CTAGCGG6600.016.33354429
CGTCGTA5550.016.2514910
CAACGTA953.347705E-416.210259
GTAGTAT953.3484685E-416.2097661
AACGCAG43900.016.13646
TACGAAT6700.016.0897641
ATACGAA6700.016.0897640
TCATATA1105.625566E-515.99924852
TAACCTA1259.446036E-615.8397315
TAGCGGC6950.015.82801630
Produced by FastQC (version 0.11.5)