##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1671227
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300061571527984	32.0	32.0	32.0	32.0	32.0
2	31.40457998823619	32.0	32.0	32.0	32.0	32.0
3	31.479921040050215	32.0	32.0	32.0	32.0	32.0
4	31.56586507996819	32.0	32.0	32.0	32.0	32.0
5	31.512306227699767	32.0	32.0	32.0	32.0	32.0
6	35.10642420209822	36.0	36.0	36.0	36.0	36.0
7	35.120162012700845	36.0	36.0	36.0	36.0	36.0
8	35.07355374225045	36.0	36.0	36.0	36.0	36.0
9	35.18663473005163	36.0	36.0	36.0	36.0	36.0
10	35.03458357242912	36.0	36.0	36.0	36.0	36.0
11	35.183552563475814	36.0	36.0	36.0	36.0	36.0
12	35.08315207928067	36.0	36.0	36.0	36.0	36.0
13	35.12577166357413	36.0	36.0	36.0	36.0	36.0
14	35.06707586701268	36.0	36.0	36.0	36.0	36.0
15	35.04492926454635	36.0	36.0	36.0	36.0	36.0
16	35.04946964116784	36.0	36.0	36.0	36.0	36.0
17	35.0061230461212	36.0	36.0	36.0	36.0	36.0
18	35.01065863584061	36.0	36.0	36.0	36.0	36.0
19	35.00891560512127	36.0	36.0	36.0	36.0	36.0
20	34.998423912490644	36.0	36.0	36.0	36.0	36.0
21	34.994295209447905	36.0	36.0	36.0	36.0	36.0
22	34.98546158002473	36.0	36.0	36.0	36.0	36.0
23	34.92501617075359	36.0	36.0	36.0	32.0	36.0
24	34.9006382735559	36.0	36.0	36.0	32.0	36.0
25	34.86655373566846	36.0	36.0	36.0	32.0	36.0
26	34.81188731393162	36.0	36.0	36.0	32.0	36.0
27	34.81505265293105	36.0	36.0	36.0	32.0	36.0
28	34.7893146771803	36.0	36.0	36.0	32.0	36.0
29	34.75672006256481	36.0	36.0	36.0	32.0	36.0
30	34.734713476984275	36.0	36.0	36.0	32.0	36.0
31	34.73006359997774	36.0	36.0	36.0	32.0	36.0
32	34.69082297018897	36.0	36.0	36.0	32.0	36.0
33	34.66623684275086	36.0	36.0	36.0	32.0	36.0
34	34.65425762029934	36.0	36.0	36.0	32.0	36.0
35	34.60644963251551	36.0	36.0	36.0	32.0	36.0
36	34.57598040242289	36.0	36.0	36.0	32.0	36.0
37	34.57260084955545	36.0	36.0	36.0	32.0	36.0
38	34.531515467378156	36.0	36.0	36.0	32.0	36.0
39	34.537165806919106	36.0	36.0	36.0	32.0	36.0
40	34.497675061496736	36.0	36.0	36.0	32.0	36.0
41	34.48280335346425	36.0	36.0	36.0	32.0	36.0
42	34.41269857416138	36.0	36.0	36.0	32.0	36.0
43	34.40740126864873	36.0	36.0	36.0	32.0	36.0
44	34.33777338446543	36.0	36.0	36.0	32.0	36.0
45	34.29372371317601	36.0	36.0	36.0	32.0	36.0
46	34.275684871055816	36.0	36.0	36.0	32.0	36.0
47	34.237006702261276	36.0	36.0	36.0	32.0	36.0
48	34.187458675571904	36.0	36.0	36.0	32.0	36.0
49	34.16351040283576	36.0	36.0	36.0	32.0	36.0
50	33.55823356132949	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	7.0
20	21.0
21	71.0
22	186.0
23	557.0
24	1479.0
25	3185.0
26	6565.0
27	11707.0
28	19370.0
29	29274.0
30	41890.0
31	58984.0
32	86152.0
33	138587.0
34	291804.0
35	981386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19109682901579	17.700064030064212	11.775906743502745	26.332932397417252
2	16.252957416788433	20.26481221525327	36.29811000573233	27.184120362225965
3	17.753585248647248	24.390880211677317	28.786343893065137	29.0691906466103
4	12.213720817100251	15.633603334555987	35.67253281570966	36.4801430326341
5	14.44453685824846	36.825637690152206	32.98247335640222	15.747352095197122
6	34.58578861959857	35.50487546851888	16.489172008061157	13.420163903821392
7	30.71278211325099	30.38457994310764	20.579083858197514	18.32355408544386
8	28.953098531797295	31.91840486062037	19.083044972346666	20.04545163523567
9	27.954822881761608	13.335686931546196	18.1676041168023	40.5418860698899
10	16.076346940814844	26.19438695127431	31.05953579824953	26.669730309661304
11	37.76111802884946	20.64788326181901	22.287516896268432	19.303481813063097
12	25.018279993250463	23.343282117439532	28.379165953617285	23.25927193569272
13	29.60241500943924	18.980801392998504	25.338930059866627	26.077853537695628
14	24.239477746795753	19.024006414475654	24.901509077201087	31.835006761527506
15	25.55005107221737	26.597644873659842	22.127045656228628	25.725258397894162
16	26.313002331247816	25.234800216370434	23.134283703763984	25.317913748617766
17	24.323027332612508	25.37099987015528	24.83708077957094	25.468892017661275
18	25.36032038850637	24.268244594820192	25.761524059355406	24.609910957318032
19	26.042797323641448	24.248366169506696	25.194019240964415	24.51481726588744
20	26.316510662589	23.334611842746934	24.668729434896843	25.680148059767223
21	27.60169623875153	23.42404712226406	24.14465539391118	24.829601245073228
22	26.598138133322486	23.343341953751317	24.634908743640896	25.423611169285305
23	24.747055588831373	23.272321906730244	25.407711865065995	26.572910639372388
24	25.219256123823392	24.367781840680365	24.868434358393486	25.544527677102764
25	25.33276847212823	23.672710801235276	25.12585215356326	25.868668573073233
26	25.333063659080686	24.433684069432342	25.296202858866216	24.937049412620755
27	25.815997103730144	24.047776484562704	24.559590453080727	25.576635958626426
28	25.1294460910063	23.77840767993949	25.52055190439689	25.57159432465732
29	25.065911274986746	24.02072540100264	25.267983336385058	25.645379987625557
30	24.898304537071724	24.30440782064738	25.608228027616647	25.18905961466425
31	25.520854526958292	24.19921010113099	24.391897552510322	25.888037819400395
32	25.38500111299669	24.127614046257577	24.288280503888306	26.199104336857427
33	24.98514495116833	23.75515289181276	24.904003173864208	26.355698983154703
34	25.745991568755926	23.843187237645594	25.058686604853502	25.352134588744978
35	26.42683954293264	23.638563502587402	25.051101739134594	24.88349521534536
36	25.24757659480378	24.453907278982275	24.532957054010566	25.76555907220338
37	26.173514724845994	24.303371021838192	24.552659687461666	24.970454565854155
38	25.370549560498812	24.014735213174085	24.913903963798674	25.700811262528433
39	25.974472821273846	23.99303477823787	24.333217645228462	25.69927475525982
40	26.050796291254407	24.239805886242397	24.707019270756977	25.00237855174622
41	24.898544133750605	24.84169773272617	25.079435365642034	25.180322767881187
42	26.37132165069609	24.692286611161574	24.511338572688572	24.425053165453765
43	25.603383435026668	23.684105023872053	24.858350371978	25.854161169123284
44	25.23257170724118	24.386655138059066	24.442243770213707	25.938529384486042
45	25.369403891560943	24.642324165901748	24.459462208728308	25.528809733809
46	25.17125377871283	24.556906278347824	24.57820828576284	25.693631657176503
47	25.34814907895249	24.325467704247558	24.97919153659507	25.347191680204883
48	26.33970243993509	24.798886064557514	23.82066213816246	25.04074935734494
49	25.297073217725245	25.01673925830276	24.06623660186175	25.61995092211024
50	25.31959071325993	25.10734801340354	24.05008377213978	25.52297750119675
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	40.0
2	46.0
3	125.5
4	205.0
5	254.5
6	304.0
7	291.0
8	278.0
9	409.0
10	540.0
11	819.0
12	1098.0
13	1962.0
14	2826.0
15	3843.5
16	4861.0
17	5188.5
18	5516.0
19	5439.0
20	5362.0
21	5728.0
22	6094.0
23	6580.5
24	7067.0
25	7433.5
26	7800.0
27	9306.5
28	10813.0
29	12627.5
30	14442.0
31	16692.0
32	18942.0
33	22402.5
34	25863.0
35	30213.5
36	34564.0
37	42987.5
38	51411.0
39	58541.5
40	65672.0
41	73066.5
42	80461.0
43	83357.5
44	86254.0
45	97010.5
46	107767.0
47	115520.0
48	123273.0
49	128666.0
50	134059.0
51	130733.5
52	127408.0
53	126569.5
54	125731.0
55	127285.5
56	128840.0
57	126909.5
58	124979.0
59	114153.0
60	103327.0
61	90957.0
62	78587.0
63	68452.5
64	58318.0
65	49492.0
66	40666.0
67	34749.0
68	28832.0
69	26123.0
70	23414.0
71	18102.5
72	12791.0
73	10801.5
74	8812.0
75	6823.0
76	4834.0
77	4220.5
78	3607.0
79	2968.5
80	2330.0
81	1864.0
82	1398.0
83	1163.0
84	928.0
85	677.5
86	427.0
87	297.0
88	167.0
89	131.5
90	96.0
91	78.5
92	61.0
93	52.5
94	44.0
95	38.0
96	32.0
97	29.0
98	26.0
99	23.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008197569809487281
2	2.991813799082949E-4
3	2.3934510392663593E-4
4	0.0
5	0.0
6	1.7950882794497694E-4
7	5.983627598165898E-5
8	0.0
9	0.0016155794515047928
10	8.377078637432257E-4
11	0.0
12	5.983627598165898E-5
13	7.180353117799078E-4
14	4.1885393187161287E-4
15	0.003829521662826175
16	0.0011368892436515207
17	0.0
18	3.590176558899539E-4
19	5.983627598165898E-5
20	5.983627598165898E-4
21	0.0
22	5.983627598165898E-5
23	5.983627598165898E-4
24	4.7869020785327185E-4
25	0.0011967255196331796
26	0.004487720698624424
27	0.006701662909945806
28	0.004068866766752811
29	0.0024532873152480184
30	0.003650012834881198
31	0.007599207049670691
32	0.003769685386844516
33	0.0046672295265694
34	0.0037098491108628574
35	0.003051650075064608
36	0.00765904332565235
37	0.004308211870679447
38	0.00765904332565235
39	0.004009030490771151
40	0.0025131235912296775
41	0.003410667730954562
42	0.0017352520034681104
43	0.0015557431755231336
44	0.0013163980715964977
45	0.0012565617956148388
46	0.0013762343475781566
47	0.0019147608314130874
48	0.002094269659358065
49	3.590176558899539E-4
50	0.0016155794515047928
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1671227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.627601277582144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.10241389527205	32.89860192364672
2	14.704258659626642	13.41840102407758
3	5.375906752497029	7.358691894251878
4	2.5011148443671747	4.564794834729096
5	1.3308364789882645	3.0361438114468937
6	0.846080489156748	2.316277392479146
7	0.571916212735772	1.8266615443224856
8	0.3977314946427736	1.4518027242477816
9	0.2870220221408877	1.17865137457168
>10	1.668259214531612	13.740316459962113
>50	0.11149823187441253	3.474615395741675
>100	0.08738568855145509	8.40623146400196
>500	0.011220011248275756	3.5932319560148507
>1k	0.004356004366977646	2.73557820050611
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2055	0.12296354714230921	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1943	0.1162618842323634	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1921	0.11494548616076691	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1806	0.10806431442287613	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1732	0.10363643000023337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1967255196331796E-4
2	0.0	0.0	0.0	0.0	1.1967255196331796E-4
3	0.0	0.0	0.0	0.0	1.1967255196331796E-4
4	0.0	0.0	0.0	0.0	1.1967255196331796E-4
5	0.0	0.0	0.0	0.0	1.1967255196331796E-4
6	0.0	0.0	0.0	0.0	1.1967255196331796E-4
7	0.0	0.0	0.0	0.0	1.1967255196331796E-4
8	0.0	0.0	0.0	0.0	1.1967255196331796E-4
9	0.0	0.0	0.0	0.0	1.1967255196331796E-4
10	0.0	0.0	0.0	0.0	2.3934510392663593E-4
11	0.0	0.0	0.0	0.0	2.3934510392663593E-4
12	0.0	0.0	0.0	0.0	7.180353117799078E-4
13	0.0	0.0	0.0	0.0	7.778715877615668E-4
14	0.0	0.0	0.0	0.0	8.377078637432257E-4
15	0.0	0.0	0.0	5.983627598165898E-5	0.0012565617956148388
16	0.0	0.0	0.0	5.983627598165898E-5	0.0013163980715964977
17	0.0	0.0	0.0	5.983627598165898E-5	0.0013163980715964977
18	0.0	0.0	0.0	5.983627598165898E-5	0.0016155794515047926
19	0.0	0.0	0.0	2.3934510392663593E-4	0.0016155794515047926
20	0.0	0.0	0.0	2.3934510392663593E-4	0.0017352520034681106
21	0.0	0.0	0.0	3.590176558899539E-4	0.0020344333833764053
22	0.0	0.0	0.0	6.581990357982489E-4	0.0020344333833764053
23	0.0	0.0	0.0	0.0019147608314130874	0.0020344333833764053
24	0.0	0.0	0.0	0.003231158903009585	0.0020344333833764053
25	0.0	0.0	0.0	0.003949194214789493	0.0020344333833764053
26	0.0	0.0	0.0	0.004667229526569401	0.0020942696593580644
27	0.0	0.0	0.0	0.0062229727020925345	0.0020942696593580644
28	0.0	0.0	0.0	0.009274622777157142	0.0022737784873030414
29	0.0	0.0	0.0	0.013403325819891612	0.0022737784873030414
30	0.0	0.0	0.0	0.023336147632847005	0.0022737784873030414
31	0.0	0.0	0.0	0.044278844226427645	0.0022737784873030414
32	0.0	0.0	0.0	0.06468301433617336	0.0022737784873030414
33	0.0	0.0	0.0	0.08395029520226756	0.0022737784873030414
34	0.0	0.0	0.0	0.10758562421502285	0.0022737784873030414
35	0.0	0.0	0.0	0.13355456799106286	0.0022737784873030414
36	0.0	0.0	0.0	0.17328585524288442	0.0022737784873030414
37	0.0	0.0	0.0	0.22731801245432248	0.0022737784873030414
38	0.0	0.0	0.0	0.30857567523741536	0.0023336147632847005
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3565	0.0	19.931742	1
GTATAGA	155	3.45608E-11	19.870035	1
GTTATAT	140	4.087269E-9	18.856258	1
ACCGTCG	500	0.0	18.479687	8
ATACCGT	525	0.0	18.018742	6
TACGCTC	75	0.001290521	17.60128	34
TACCGTC	560	0.0	17.285421	7
CGGTCCA	680	0.0	16.82274	10
CGCAATA	655	0.0	16.795116	36
GTATATA	200	7.2759576E-11	16.499226	1
CTAGCGG	660	0.0	16.333544	29
CGTCGTA	555	0.0	16.25149	10
CAACGTA	95	3.347705E-4	16.21025	9
GTAGTAT	95	3.3484685E-4	16.209766	1
AACGCAG	4390	0.0	16.1364	6
TACGAAT	670	0.0	16.08976	41
ATACGAA	670	0.0	16.08976	40
TCATATA	110	5.625566E-5	15.9992485	2
TAACCTA	125	9.446036E-6	15.839731	5
TAGCGGC	695	0.0	15.828016	30
>>END_MODULE