FastQCFastQC Report
Thu 2 Feb 2017
SRR4064199_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064199_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13189
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1160.8795208127985442No Hit
TATCAACGCAGAGTACTTTTTTTTT720.5459094700128895No Hit
GTACTTTTTTTTTTTTTTTTTTTTT330.25020850708924103No Hit
GAGTACTTTTTTTTTTTTTTTTTTT280.21229812722723482No Hit
GGTATCAACGCAGAGTACTTTTTTT270.20471605125483358No Hit
AGGTTATCTAGAGTCACCAAAGCCG250.18955189931003108No Hit
ACGCAGAGTACTTTTTTTTTTTTTT250.18955189931003108No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT200.15164151944802487No Hit
GAACTACGACGGTATCTGATCGTCT140.10614906361361741No Hit
AGATAACCTCGGGCCGATCGCACGC140.10614906361361741No Hit
GTGTAAAGGTATAAGGGAGCTTGAC140.10614906361361741No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG140.10614906361361741No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)