##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064199_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13189
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.058457805747214	32.0	32.0	32.0	14.0	32.0
2	27.476457654105694	32.0	21.0	32.0	14.0	32.0
3	27.698460838577603	32.0	21.0	32.0	14.0	32.0
4	27.484570475396165	32.0	21.0	32.0	14.0	32.0
5	27.614451436803396	32.0	21.0	32.0	14.0	32.0
6	30.92387595723709	36.0	21.0	36.0	14.0	36.0
7	30.821138827811055	36.0	21.0	36.0	14.0	36.0
8	30.69633785730533	36.0	21.0	36.0	14.0	36.0
9	30.99090150883312	36.0	32.0	36.0	14.0	36.0
10	29.806581241944045	36.0	21.0	36.0	14.0	36.0
11	31.240655091364015	36.0	32.0	36.0	14.0	36.0
12	30.56213511259383	36.0	21.0	36.0	14.0	36.0
13	30.84145879141709	36.0	32.0	36.0	14.0	36.0
14	30.341496701796952	36.0	21.0	36.0	14.0	36.0
15	30.272423989688377	36.0	21.0	36.0	14.0	36.0
16	30.20274471150201	36.0	21.0	36.0	14.0	36.0
17	30.07445598604898	36.0	21.0	36.0	14.0	36.0
18	30.1420881037228	36.0	21.0	36.0	14.0	36.0
19	30.11661232845553	36.0	21.0	36.0	14.0	36.0
20	29.928501023580257	36.0	21.0	36.0	14.0	36.0
21	30.066191523239063	36.0	21.0	36.0	14.0	36.0
22	29.948669345666843	36.0	21.0	36.0	14.0	36.0
23	29.635226324967775	36.0	21.0	36.0	14.0	36.0
24	29.771627871711274	36.0	21.0	36.0	14.0	36.0
25	28.79232693911593	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	1.0
13	0.0
14	15.0
15	27.0
16	67.0
17	116.0
18	189.0
19	265.0
20	327.0
21	386.0
22	417.0
23	446.0
24	455.0
25	486.0
26	534.0
27	580.0
28	593.0
29	664.0
30	676.0
31	865.0
32	1076.0
33	1223.0
34	1786.0
35	1993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79874118449989	22.196102221885187	11.45825434139683	24.546902252218096
2	19.20539843809235	20.100083402835697	36.818560921980435	23.875957237091516
3	19.554173932822806	24.785806353779666	28.6450830237319	27.01493668966563
4	13.010842368640535	16.316627492607477	36.6062627947532	34.06626734399879
5	13.952077646345163	37.70094024871095	33.454655747649376	14.892326357294511
6	32.12525589506407	37.341724164076126	17.089999241792402	13.443020699067404
7	28.359114346375492	30.990294206854717	21.84561722778283	18.80497421898696
8	26.971489232635733	32.76463451622687	19.760388231725813	20.503488019411588
9	27.089337175792505	13.65842560291218	19.156681328681934	40.095555892613376
10	15.304110420142575	27.18034278780525	32.36766267253147	25.147884119520704
11	36.46844660194174	21.53367718446602	23.270631067961165	18.72724514563107
12	24.302184466019416	24.552487864077673	29.535800970873787	21.60952669902913
13	28.363415743970876	20.112240254815713	25.564993174579097	25.95935082663431
14	22.624194160030335	20.43230944254835	27.28100113765643	29.66249525976488
15	24.072810011376564	27.243079256731136	23.814941221084567	24.869169510807737
16	24.400788834951456	26.479065533980584	24.173240291262136	24.946905339805824
17	22.513843586437076	25.745278009557765	26.670712280967912	25.070166123037247
18	23.4375	24.73452669902913	27.745752427184467	24.082220873786408
19	24.173240291262136	24.840716019417474	27.46510922330097	23.520934466019416
20	24.302184466019416	25.128944174757283	26.570084951456312	23.99878640776699
21	25.536594615092913	24.338263177853623	26.788016685627607	23.337125521425865
22	24.23391990291262	25.151699029126213	26.72936893203883	23.885012135922327
23	23.257225214291132	24.31919896836835	27.52787681104453	24.895699006295988
24	23.185437997724687	26.03716344330679	26.35570724307926	24.42169131588927
25	22.959648058252426	24.97724514563107	27.64714805825243	24.415958737864077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	6.5
3	11.0
4	11.0
5	11.0
6	27.5
7	44.0
8	44.0
9	44.0
10	43.5
11	43.0
12	43.0
13	43.0
14	41.0
15	39.0
16	39.0
17	39.0
18	80.0
19	121.0
20	121.0
21	121.0
22	186.0
23	251.0
24	251.0
25	251.0
26	379.0
27	507.0
28	507.0
29	507.0
30	620.5
31	734.0
32	734.0
33	734.0
34	844.5
35	955.0
36	955.0
37	955.0
38	1059.0
39	1163.0
40	1163.0
41	1163.0
42	1309.5
43	1456.0
44	1456.0
45	1456.0
46	1585.5
47	1715.0
48	1715.0
49	1715.0
50	1705.5
51	1696.0
52	1696.0
53	1696.0
54	1531.5
55	1367.0
56	1367.0
57	1367.0
58	1255.0
59	1143.0
60	1143.0
61	1143.0
62	983.0
63	823.0
64	823.0
65	823.0
66	692.5
67	562.0
68	562.0
69	562.0
70	404.5
71	247.0
72	247.0
73	247.0
74	192.0
75	137.0
76	137.0
77	137.0
78	113.0
79	89.0
80	89.0
81	89.0
82	68.0
83	47.0
84	47.0
85	47.0
86	31.0
87	15.0
88	15.0
89	15.0
90	11.0
91	7.0
92	7.0
93	7.0
94	5.0
95	3.0
96	3.0
97	3.0
98	7.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015164151944802487
2	0.0
3	0.0
4	0.0
5	0.0075820759724012435
6	0.0
7	0.0075820759724012435
8	0.0075820759724012435
9	0.02274622791720373
10	0.02274622791720373
11	0.03791037986200622
12	0.03791037986200622
13	0.02274622791720373
14	0.030328303889604974
15	0.030328303889604974
16	0.03791037986200622
17	0.04549245583440746
18	0.03791037986200622
19	0.03791037986200622
20	0.03791037986200622
21	0.030328303889604974
22	0.03791037986200622
23	0.04549245583440746
24	0.030328303889604974
25	0.03791037986200622
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	13189.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.79877170369247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.48709791445741	80.21078171203276
2	3.8264404383174266	6.566077792099477
3	1.1311417462000708	2.9115171734020775
4	0.49487451396253096	1.6983850178178785
5	0.32697066101095795	1.40268405489423
6	0.22092612230470132	1.1373113958601866
7	0.15906680805938495	0.9553415725225566
8	0.06185931424531637	0.42459625445446963
9	0.07069635913750442	0.5459094700128895
>10	0.20325203252032523	2.7219652740920464
>50	0.008837044892188053	0.5459094700128895
>100	0.008837044892188053	0.8795208127985442
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	116	0.8795208127985442	No Hit
TATCAACGCAGAGTACTTTTTTTTT	72	0.5459094700128895	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	33	0.25020850708924103	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	28	0.21229812722723482	No Hit
GGTATCAACGCAGAGTACTTTTTTT	27	0.20471605125483358	No Hit
AGGTTATCTAGAGTCACCAAAGCCG	25	0.18955189931003108	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	25	0.18955189931003108	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	20	0.15164151944802487	No Hit
GAACTACGACGGTATCTGATCGTCT	14	0.10614906361361741	No Hit
AGATAACCTCGGGCCGATCGCACGC	14	0.10614906361361741	No Hit
GTGTAAAGGTATAAGGGAGCTTGAC	14	0.10614906361361741	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	14	0.10614906361361741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE